1. ABC analysis is based upon the principle that A) all items in inventory must be monitored very closely. B) more time should be spent on class "C" items because there are more of them. C) an item is critical if its usage is high. D) there are usually a few critical items, and many items which are less critical. E) as with grade distributions in many undergraduate courses, there should be more medium-level "B" items than either "A" or "C"items. Your answer: L ) 2. The graph below plots two different types of costs as a function of the order quantity in the EOQ model. Line B is a plot of the A) Shortage Costs B) Demand C) Order Costs D) Carrying or Holding Costs Annual Order Quantity. O Your answer:( Your answer: L ]

Answers

Answer 1

ABC analysis is based upon the principle that, option D. There are usually a few critical items, and many items which are less critical.

In the EOQ model, Line B is a plot of the option D. Carrying or Holding Costs.

What is ABC analysis?

ABC analysis is a technique used in inventory management to divide items into three categories based on their relative importance. The categories are typically called A, B, and C, with A items being the most critical and C items being the least critical.

Also, the Economic Order Quantity (EOQ) model is a mathematical model used to determine the optimal order quantity that a company should order to minimize the total costs associated with holding and ordering inventory. The EOQ model considers two types of costs: carrying or holding costs and order costs. Line B in the graph is a plot of the carrying or holding costs, which are the costs associated with storing and maintaining inventory over time. The horizontal axis represents the annual order quantity, which is the amount of inventory that a company orders in a year.

Therefore, the correct answer is as given above

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1. ABC Analysis Is Based Upon The Principle That A) All Items In Inventory Must Be Monitored Very Closely.

Related Questions

What are the 4 importance of preamble?

Answers

The Preamble states the goals of the Constitution, which include guaranteeing justice, defending individual liberties, attaining social equality, and fostering a spirit of brotherhood among citizens.

What is meant by preamble?a statement to begin with. Particularly: the first clause of a constitution or law, which typically explains the purpose and goals of the rule of law.: an opening occurrence or circumstance. The Preamble serves as the Constitution's introductory or prologue paragraph. It outlines the Constitution's fundamental tenets and goals. The Preamble is considered the soul of the Constitution since it comprises the guiding principles that were used to create and develop the whole document. The Indian Constitution's Preamble outlines the document's guiding principles and identifies the sources of its power. It was approved by the Constituent Assembly on November 26, 1949, and it became operative on January 26, 1950, which is recognized as India's Republic Day.

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How did the pigs manipulate the animals?

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The pigs manipulated the animals in a variety of ways. First, they used their superior intelligence to create loyalty among the other animals by convincing them that they were the only ones who could lead the farm to success.

They also instilled a sense of fear and suspicion among the animals, convincing them that any animal that disagreed with their plans was a traitor. Through their clever language manipulation, the pigs were also able to convince the animals that the commandments of Animalism were sacred and any animal that violated them was a traitor. In addition, the pigs used their positions of power to reward animals that obeyed their commands, while punishing those that disobeyed. This created an atmosphere of obedience and loyalty that allowed the pigs to remain under control.

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Do interest rates rise or fall during a recession?

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Getting an Adjustable-Rate Mortgage (ARM) During a recession, credit requirements are often stringent, making it difficult for some borrowers to get the best loans and interest rates.

When there is a recession, how do interest rates change?

When loan demand slows, bond prices rise, and the central bank loosens monetary policy, interest rates typically fall. During late downturns, the Central bank has stopped term rates and facilitated credit access for metropolitan and corporate borrowers.

The price of money is the most significant economic price. The expansion and contraction of the business cycle is arguably driven by interest rates. Market rates are a reflection of borrowers' demand for credit and the supply of credit, which in turn is a reflection of shifting preferences between saving money and spending it.

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when are tokens exchanged for backup reinforcers? group of answer choices when the client accumulates a specific number of tokens at a time and place established by the developers of the token economy at the end of the day whenever the client wants to exchange tokens

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The tokens exchanged for backup reinforcers  ,when the user reaches a predetermined threshold of tokens at a time and location decided upon by the token economy's creators at the conclusion of the day.

What is  token economy ?A contingency management system based on the methodical reinforcement of target behaviour is known as a token economy. Symbols or tokens that can be traded for other reinforcers are known as reinforcers.A token economy can be categorised under applied behaviour analysis and is based on the concepts of operant conditioning and behavioural economics. Token economies are employed with kids and adults in real-world situations, but they have been effectively modelled with pigeons in lab settings. For tokens to be useful, they must be employed as reinforcers. Tokens are items or symbols that can be used to exchange for goods, services, or benefits (back-up reinforcers). A variety of tokens have been employed in practical situations, including money, checkmarks, pictures of tiny suns or stars, points on a counter

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What are the rules for rejecting shipment?

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The rules for rejecting shipment are differ in the products and services manufacturing process. The rules for rejecting shipment plays key role in the supply chain.

The shipment is also known as packaging process of the products or goods just after the process of manufacturing from the raw materials in the supply chain.

The rules for rejecting shipment are if the delivery of the orders are delayed by the 8-9 hours then the  retailer or purchaser canceled the orders and the products should in the box or properly packed.

In the B2C business organization, if the process of shipment is not according to the rules then the business faces lots of loss by reject of the  orders.

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a business is employing inputs such that the marginal product of labor is 50 and the marginal product of capital is 100. the price of labor is $20, and the price of capital is $50. if the business wants to minimize costs while keeping output constant, then it should

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Less labor and more capital should be used by the company if it wishes to keep costs low and production steady.

What is cost minimization?

The primary principle that producers employ to determine what combination of labor and capital delivers output at the lowest cost is cost minimization. To put it another way, what would be the most economical way to deliver goods and services while keeping an acceptable level of quality.

Costs are reduced so that the marginal product of labor divided by the wage (w) equals the marginal product of capital divided by the capital rental price at the levels of capital and labor (r).

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Why do companies issue stock dividends?

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Companies issue stock dividends because they don't want to deplete their cash reserves.

If companies does not want to deplete its cash reserves, it may issue stock dividends rather than a cash dividend. Dividends, whether in cash or stock, represent the shareholders' share of the company's profit. They are also a benefit of holding the stock rather than selling it. Dividends allow companies to share their profits with shareholders, which allows them to thank shareholders for their ongoing support through higher returns and incentivizes them to continue holding the stocks. Dividends are the distribution of a company's profits to shareholders based on the number of shares held. Shareholders expect companies in which they invest to return profits to them, but not all companies do.

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What to do if exposed to chemicals at work?

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If you are exposed to chemicals at work you should immediately get out of the site and seek help and medical attention.

Workers are currently experiencing a health crisis as a result of exposure to dangerous substances at work. After such exposures, many of these workers have terrible illnesses, malignancies, and poisonings, or they pass away in fires or explosions. Inform a manager of your exposure as soon as you can.

Explicitly describe how you were found. Any witnesses to your exposé should be noted, and you should acquire their identities. If you require medical assistance, you should obtain it. There are several short- and long-term health problems that can result from exposure to common workplace chemicals, including poisoning, skin rashes, lung, kidney, and liver illnesses.

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A supply curve slopes upward because
O the quantity supplied of most goods and services increases over time.
O an increase in price gives producers an incentive to supply a larger quantity.
O as more is produced, total cost of production falls.
O an increase in input prices increases supply

Answers

A supply curve slopes upward because an increase in price gives the producers an incentive to supply a larger quantity. The Option B is correct.

What is a supply curve?

A supply curve refers to a graphic representation of the correlation between the cost of a good and the quantity supplied for a given period. In an illustration, the price will appear on the left vertical axis while the quantity supplied will appear on the horizontal axis.

Usually, the supply curve slopes upward because as a product's price rises, the business would tend to be more willing to make it. As businesses are efficient and would exhaust the cheapest production inputs first, then, the cost of production tends to rise as output increases.

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Select the true statement about the bankruptcy process. O A bankruptcy reorganization plan is voted on by a company's shareholders. O A company receives a stay from any collections activity after filing a bankruptcy petition. O Corporations file bankruptcy petitions with the states. O A Chapter 7 bankruptcy allows a company to restructure its debt. A security that falls above the security market line is O correctly valued for its level of risk O over-valued for its level of risk o attractive for a company raising capital o attractive for an investor

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The true statement of the bankruptcy process is that corporations file bankruptcy petitions with states.

How does bankruptcy work?

Bankruptcy provides a fresh start as it wipes out unmanageable obligations and gives creditors chances to receive payment based on the remaining assets, which the firm or the individual will sell them off.

According to theory, an opportunity of being declared bankrupt will help the whole economy as it provides businesses and people another chance to access credit and gives creditors a share of the debt repayment. A debtor will be released from debt obligations accumulated before filing bankruptcy when the bankruptcy proceedings are concluded successfully.

The federal courts in the US will oversee all bankruptcy cases. The bankruptcy judge will make all the judgments in federal bankruptcy cases, which include whether or not a debtor is entitled to file and if or not their debts should be forgiven.

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What makes a community bank different?

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Community banks prioritize offering traditional banking services to their surrounding areas. They collect the majority of their core deposits locally and lend to a large number of neighbourhood companies. They are frequently referred to as "relationship" bankers as opposed to "transactional" bankers because of this.

A depository institution that is typically run and owned locally is referred to as a community bank. [Needs citation] Community banks frequently concentrate on the requirements of the local businesses and households where they have offices and branches. People who are knowledgeable about the needs of local families, businesses, and farmers make the lending decisions. [Needs citation] Employees frequently live in the communities they work in. Community banks are not precisely defined in the United States. [Needs citation] The majority of agencies define this phrase in terms of the size of total assets, ranging from less than $1 billion (Office of the Comptroller of the Currency) to less than $10 billion (Federal Reserve Board and Government Accountability Office). The Federal Deposit Insurance Corporation (FDIC) places extra standards on organizations that are designated as community banks in addition to size (as determined by assets). In the period from 1985 to 2004, community banks made up nearly 94% of all commercial banks in the country, although their share of total national deposits fell from about 25.89% in 1985 to 13.55% in 2003. Economists and decision-makers have become more concerned about the loss in community banking in the United States, and some of them have claimed that community banks are crucial to macroeconomic stability.

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Which of the following is NOT a characteristic of the management control system?

a. It aids and coordinates the process of making decisions.
b. It encourages short-term profitability.
c. It motivates individuals throughout the organization to act in concert.
d. It coordinates forecasting sales and cost-driver activities, budgeting, and measuring and evaluating performance.

Answers

The purpose of a management control system is to enhance group decisions in a way that is economically viable. So the option b “It encourages short-term profitability” is NOT a characteristic of the management control system.

In order to facilitate and coordinate the process of making planning and control choices throughout the organization and to direct the behavior of its managers and employees, management control systems are a way to collect and use information.

The purpose of a management control system is to enhance group decisions in a way that is economically viable. The control system ought to be created with the goal of achieving organizational cohesion by utilizing the wide range of skills that each member of the workforce possesses.

The pursuit of both short-term and long-term objectives necessitates coordination in order to establish unity in variety, which is a constant demand of management control.

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green clean, a maker of home cleaning products, is considering the purchase of well heeled, a women's shoe manufacturer, for the main purpose of diversification. what might you say to the executives at green clean?

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Simple Green lives up to Good Housekeeping's claim that it's the best all-purpose cleaner for everyday use.

What are the 3 Steps of Green cleaning?

1. Consider your current workspace

When thinking about introducing green cleaners to your office, keep these things in mind.

2. Replace rusted or difficult-to-clean items

Eco-friendly office supplies are not only good for the environment, but they can wind up saving you money over time. Additionally, there's no need to spend a fortune up front. Simple modifications have the same impact.

3. Use green cleaning products instead of chemical ones.

Given the subject, this one might seem obvious, yet it's the most crucial. Eliminate those dangerous cleaners in a responsible and safe manner. Next, start the green revolution in your office or workplace by bringing in some eco-friendly cleaning supplies. You can change your commercial to get started.

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if the firm has 200,000 common shares outstanding, its earnings per share eps is what while it's dividends per share dps s what

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The firm having common shares would then have earnings per share as $2.50 and dividends per share as $0.50.

For instance, if the business generated a net income of

$500,000 and paid dividends of $100,000,

the EPS would be

$500,000 / 200,000

[tex] \frac{500000}{200000} [/tex]

= $2.50

and the DPS would be $100,000 / 200,000

[tex] \frac{100000}{200000} [/tex]

= $0.50.

A financial measure called earnings per share (EPS) is computed by dividing a company's net income by the total number of outstanding common shares. It displays the profit generated by a business for each share of its stock.

A financial ratio called dividends per share (DPS) is computed by dividing a company's total dividend payments by the number of outstanding ordinary shares. It displays the dividend amount paid by a firm for each share of its stock.

You would need to know the company's net income and total dividends paid in order to compute the EPS and DPS for a corporation with 200,000 outstanding common shares. These numbers can then be multiplied by 200,000 to yield the EPS and DPS, respectively.

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you are hired as a consultant to a monopolistically competitive firm. the firm reports the following information about its price, marginal cost, and average total cost. can the firm possibly be maximizing profit? if not, what should it do to increase profit? if the firm is maximizing profit, is the market in a long-run equilibrium? if not, what will happen to restore long-run equilibrium?

Answers

If marginal revenue equals marginal cost, the firm may be maximizing profit. Because price equals average total cost, the company is in long-run equilibrium. As a result, the company makes no economic profit.

What is the best way to explain equilibrium?

Equilibrium is the situation in which business demand and supply balance each other, resulting in stable prices. In general, an oversupply of services or goods causes prices to fall, resulting in increased demand, whereas an undersupply or shortage creates prices to rise, resulting in decreased demand.

What are the two things required for equilibrium?

In a chemical reaction, the two conditions for balance are that neither the resulting force or torque must act on the matter.

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What are the 5 tips to be followed in a group discussion?

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Group Discussion involves sharing ideas with alternative participants within the cluster. Each participant shares his/her thoughts within the cluster, that makes the variations within the ideas.

Word is wide used for the enlisting method. It helps panellist to pick out some candidates from the cluster. Ask dominant participants to permit others to talk. Provide all participants a voice- at the beginning highlight the worth of a diversity of views as a necessary a part of the method. reconsider constructive and harmful cluster behaviors at the beginning of the course / workshop.

It improves your thinking, listening and speaking skills. It conjointly promotes your confidence level. it's a good tool in downside finding, higher cognitive process and personality test. Group Discussion skills could guarantee educational success, quality and sensible admission or job provide.

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Which of the following receive dividends if declared?

a. both stockholders
b. creditors
c. stockholders

Answers

From the following stockholders are the ones which receive dividends if declared.

An person or legal entity that the corporation registers as the legitimate owner of share of the shareholding of a public or privately held corporation is referred to as a shareholder of the corporation. A corporation's members may be alluded to as shareholders.

A person or legal entity joins a company as a shareholder when their name and other information are put in the company's register of members or shareholders. Unless mandated by law, the corporation is not compelled or allowed to inquire as to who really owns the shares in question. Generally speaking, a business cannot own its own stock.

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A work center can be a:I. machine.II. group of machines.III. department.IV. a part used to produce a product.II and IV onlyII and III onlyI and III onlyI, II, III, and IVI, II, and III only

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A work center can be a: I. machine.II. group of machines.III. department. IV. a part used to produce a product. I II and III

Work centre is a location where inputs are processed, and outputs are delivered in the form of finished goods or services. Work centres can be machines or groups of machines that can work on materials. A department can also serve as a work centre. The used part can be an input but not a work centre.Health center, illness, and health insurance among the most common benefits, as are pension payments, paid sick leave off, and retirement benefits. Advantages can be immensely important. Health coverage alone can cost thousands of dollars per month. That is why it is important to consider advantages as part of your total salary.

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________ is increasing the pace of both cultural diffusion and cultural change.

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Globalization is increasing the pace, which is of both cultural diffusion and cultural change.

Cultural change tends to occurs when people tend to integrate into their culture the gestures, traditions, material objects, or concepts of another culture which is done mainly through the cultural diffusion. Thus, globalization and technology are increasing by which the pace of cultural is changing around the world.

However, the cultural diffusion tends to describe the spread of one culture's beliefs, practices, and/or items, like food, music, or tools. Thus, it has been witnessed that globalization has its own impact on the cultures around the world.

Hence, globalization changes the way businesses, nations, and the people tend to interact.

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What are layers in container filesystems?

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A container's root filesystem is built up from layers that are stacked on top of one another.

What are container layers?

A new writable layer is added on top of the existing layers when a new container is created. It is common to refer to this layer as the "container layer." This thin layer of writable container receives all updates made to the running container, including new file creation, file modification, and file deletion.

The way Docker images are created causes layers. A new "layer," or diff of the filesystem changes since the previous step, is created at the end of each step in a Dockerfile. Because they have no impact on the filesystem, metadata instructions like LABEL and MAINTAINER do not produce layers.

A container's root filesystem is built up from layers that are stacked on top of one another. These layers are stacked and provided with an uniform view by the Docker storage driver.

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Who owns sprint backlog?

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The scrum framework states that the product owner, scrum master, and members of the development team will all share ownership of the sprint backlog. This is due to the fact that at the start of each sprint, each team member will provide specialized knowledge and insights into the project.

The product owner may be aware of new organizational goals or market realities that will make it necessary to prioritize particular user stories or fixes. Recent sprints may have taught the developers that some development tasks are taking longer than the team had planned. The team will be able to create a sprint backlog that is more realistic and strategic with the aid of all these insights.

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1) _____________ are justifiable claims that utility cannot override.Group of answer choicesobligationsstandardsrightsentitlements2) This model of management morality suggests that employees are factors of production to be used, exploited, and manipulated for gain of individual managers or the company.Group of answer choicesMoral ManagementAmoral ManagementImmoral ManagementNonmoral Management

Answers

I believe it’s Moral management………..

The cost of fire insurance for a manufacturing plant is generally considered to be
O: variable cost.
O all of these.
O product cost.
O period cost.

Answers

The cost of fire insurance for a manufacturing plant is generally considered to be: product cost

Who does clarify the items in the Product Backlog?

Answers

The product owner is in charge of the backlog even though the entire cross-functional agile team works together on it.

The organization and upkeep of the product backlog is typically the responsibility of the product owner (also known as the product manager).

Who must clearly communicate the items in the product backlog?Clearly state the items in the Product Backlog.The Product Backlog's items should be arranged in the best possible order to accomplish objectives.Make the Development Team's work as valuable as possible...Make sure that everyone can see, understand, and understand what the Scrum Team will be working on next by displaying the Product Backlog.

Who is in charge of prioritizing the backlog?

At the beginning of the project, the product owner assigns priority to the backlog but does not alter it as developers and stakeholders provide feedback. The team only includes customer-facing items on the backlog.

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What is data analytics tools?

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Data analysis tools is software & programs which collect and evaluate data about a company its clients or its competitors in order to reduce operations and help interpret patterns so decisions can be made using data.

What does data analysis entail?

Data analysis is the method of examining, cleaning, modifying, and modeling data to find pertinent information, support conclusions, and assist decision-making.

What is a data analyst's role?

A data analyst looks at data to uncover useful consumer insights and possible applications for the knowledge. They also tell the management of the business as well as other stakeholders of the this information.

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Who do I call if my Amazon package is late?

Answers

The concept mentioned here is Customer service. The answer is, we call Amazon customer service if our package is late.

However, the first step you will want to take is to engage with Amazon client service, which you can do in a couple of ways If you are going to try to get a late-delivery refund.

This operates 24/7. Dispatch Amazon's client service dispatch address  but as Insider reports, the thee-commerce company prefers to handle support via live converse or phone.

client service is the support you offer your guests — both before and after they buy and use your products or services — that helps them have an easy and pleasurable experience with you. Offering amazing client service is important if you want to retain guests and grow your business.

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What are some methods which a firm in an oligopoly might use to increase its profit?

Answers

Lowering prices, forming collusions, lowering quality of goods, etc are some methods which a firm in an oligopoly might use to increase its profit.

An oligopoly is a market in which a small number of firms recognise their interdependence in pricing and output policies. Because the number of firms is small, each firm has some market power. Automobile manufacturing is another example of an oligopoly, with the top three automakers in the United States being Ford (F), General Motors (GM), and Stellantis (the new iteration of Chrysler through mergers).

Oligopolies typically form in industries with a small number of influential players, none of whom can effectively push out the others. These industries are typically capital-intensive, with additional barriers to entry such as regulation and intellectual property protections.

If the conditions are favourable, oligopolistic firms will realise that they are best served individually not by competing tooth and nail, but by coordinating and cooperating with one another to a certain extent or in specific areas of business.

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What are the requirements for informing other employees of hazardous materials in the workplace?

Answers

The OSHA regulations include workplace safety in general, handling, mixing, storing, and disposing of items, as well as your right to know what potentially harmful components are in the products you use.

In general, if your firm handles hazardous products, you must keep records and share information with two parties: your staff and local emergency response agencies.

Depending on the nature of your firm, you may or may not have a duty to notify staff members and others. In essence, you have to Identify and discuss any potential risks associated with the chemicals you produce or import, if any. Before introducing chemicals into the workplace, make sure that warning labels are in place if you distribute chemicals.

Obtain material safety data sheets (MSDS) and display them publicly at work. Make certain that all employees are knowledgeable, trained, and knowledgeable of what to do in the event of a spill or leak.

To enforce the employer's communication duties to employees, comprehensive hazard communication strategies like the following are necessary. programmes for communicating with the population about key concerns in writing through container labelling and material safety data sheets (MSDS).

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a firm has a steady growth rate of 5% per year in its dividend and this growht rate is expected to continue indefinetly. last year's dividend was $1.20. if the investor requries a 9% return, what is the value of this stock

Answers

As a result, if the investor needs a 9% return, the stock's value will be $31.50.

What is growth rate?

For the time under consideration, divide the gap between both the beginning and ending values by the starting value to get growth rates. Annual, quarterly, monthly, and weekly are the most common time periods for growth rates. Since it tells us about an economy's size and health, GDP is important. A common indicator of the state of the economy is the real GDP growth rate. A rise in real GDP is generally regarded as a sign that the economy is doing well.

Why do we use growth rate and what is the formula of it?

Growth rates enable comparisons between entities of various sizes, such as the country and Texas. Furthermore, growth rates make it simple to compare indicators across time periods. Growth rate: A measurement of the percentage change that shows how quickly an economic indicator is rising or declining over a specific period of time.

To calculate the growth rate, subtract the current value from the previous one. The difference is then multiplied by the prior figure and divided by 100 to determine the growth rate as a percentage.

Briefing:

As we know that

The value of the stock is = Dividend × (1 + growth rate) ÷ (required rate of return - growth rate)

= $1.20 × (1 + 0.05) ÷ (9% - 5%)

= $31.50

hence, the value of the stock is $31.50

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Ashley bought an expensive pair of jeans. her friend got the same pair of jeans for almost $20 less. what tip could have helped ashley?

Answers

Option D. Look for bargains, Ashley may have used the advice to hunt for deals when making her purchase.

A bargain is something that is purchased or made available for purchase at a lower cost than its original or customary price. The most likely explanation for how Ashley's buddy was able to purchase the same pair of pants for over $20 cheaper than Ashley is that she discovered them on sale after shopping for deals. Ashley most likely might have saved money if she had searched for discounts or special offers before purchasing the pair of trousers.

Ashley bought an expensive pair of jeans. Her friend got the same pair of jeans for almost $20 less. What tip could have helped Ashley?

A. Use credit cards infrequently.

B. Buy high-quality products.

C. Think about what is most important.

D. Look for bargains.

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31 studen. JUDMH T. Salinas 22. Date Section Laboratory Report 31 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method A. Results 1. List the antimicrobials that were used for each organism. After measuring and recording the zone diam eters in millimeters for each antimicrobial, consult table 31.2 for interpretation of its sensitivity. Recor the degrees of sensitivity (R. 1. or S) in the rating column. Exchange data with other class members 1 complete the entire chart. ANTIMICROBIAL ZONE DIA. RATING (R, I, S) ANTIMICROBIAL ZONE DIA RATING (R, I, S) S. epidermidis Smarcoscens P. Nuorescens 8 Which of the following is the best conclusion that can be drawn from the data in the table?(War Funding Policies) Strategic planning for organizations is typically O A group process. O The sole province of the chief executive officer (CEO). O The work of the strategic planning department. O Something that requires consultants to be successful. How are rigid motions related to congruence? 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Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (KD) Distance Migrated (mm) 37 23 25 30 20 35 15 42 51.5 10 Distance Migrated (mm) vs. Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight (kD) 40 30 Precision Plue Protein Kaleidoscope Prestained Standards Molecular Weight 20 10 25 30 35 40 45 50 Distance Migrated (mm) STUDENT MANUAL POST-LAB ACTIVITY Procedures Generate a standard curve to calculate protein sizes The different protein bands in your gel can be defined by their different molecular masses. Indeed many proteins are named for their molecular weights. For example p53, a protein implicated in tumor progression is 53 KD in size. To determine the molecular masses of the proteins, a standard curve is created plotting the known molecular masses of the proteins in the Precision Plus Protein Kaleidoscope prestained standards against the distance they have migrated down the gel from the base of the well. A 15% polyacrylamide gel is designed to separate small proteins-proteins less than 40 KD. Your gel analysis will concentrate on this size range. Note: If a different percentage acrylamide gel or an agarose gel has been run, analyze the section of the gel that has the best separation 1. As shown in the figure below draw a line between the 37 and 25 KD bands of the prestained standards. Your gel analysis will be restricted to the proteins below this line. Stenders Actin & Myosin 4 Measure distance from base of wells to the base of the bands 250 150 100 75 50 37 25 20 Measure Standard bands between 37 and 10 kD 15 Measure fish protein bands between 30 and 10 KD 10 - Fig. 18. Image of fish muscle proteins separated by SDS-PAGE and stained with Bio-Safe Coomassie stain. Lines illustrate measurement of bands for constructing the standard curve. STUDENT MANUAL POST-LAB ACTIVITY Define the characteristics (proteins) of the different fish 4. For each fish sample that has been analyzed, determine the molecular masses of the proteins below the 25-37 KD line. Measure the distance each band has migrated from the base of its well. Find that distance on the x-axis of the standard curve. Draw a line up from the x-axis to the curve. Read across to the y-axis to determine the molecular mass. Alternatively, use graphing software to generate the standard curve. Make a line of best fit (or trend line) through the points and formulate an equation to calculate the mass of the unknown proteins on the gel. 5. Enter this data into a table with the molecular masses of the proteins for each fish (see example below). Fish Species A Distance Molecular Migrated Mass (mm) (KD) 25 32.5 26.5 31 29 28.6 36 21.7 21.2 36.5 39 18.8 44 13.9 52 6 Determine which fish have each characteristic (protein) 6. Make a table with a row for every band size you have recorded for all your fish samples and a column for each type of fish on your gel. Then make a mark in each cell of the table where the fish has that size band (see example below). -LAB ACTIVITY ENT MANUAL Species A Species B Species c Species D Species E X X X X 27.5 X X X X X X 33 X X X X Characteristic Protein Distance Molecular Migrated Mass (mm) (kDa) 25 32.5 26 31.5 26.5 31.0 30.0 28.5 29.1 29 28.6 30 27.6 30.5 27.1 32 25.6 24.7 34.5 23.2 35.5 22.2 36 21.7 36.5 21.2 37 20.7 37.5 20.2 38 19.7 38.5 19.3 39 18.8 39.5 18.3 40.5 17.3 41 16.8 41.5 16.3 42 15.8 43 14.8 44 13.9 45 12.9 46 11.9 46.5 11.4 47 10.9 47.5 10.4 51.5 6.5 52 COUNT X X X X X X X X 6.0 8 10 13 10 12 Find the number of characteristics shared by each of the fish 7. In the table below both the row and column headings are the types of fish. From the table above, separately compare the number of bands (X's) in common with every other fish sample from your gel and put those numbers into the table below, such that each fish is individually compared with every other fish. In this example, species A and B have just 2 bands in common while species B and C have 10 bands in common. Your table will be the basis for drawing your cladogram. Species A Species B Species c Species D Species E Species A 8 2 2 2 Species B 10 10 5 3 Species C 13 5 Species D 2 Species E Is direct variation the same as slope? Which cells are diploid in meiosis? What are 4 values and 12 principles of Agile? How do you solve equations on a calculator? More than one of the following may be correct. Select all correct choices. A sharp increase in capillary hydrostatic pressure would directly cause lewis structure for caffeine I need help with this