A virologist has a cell-free extract of herpes simplex virus (HSV) that has a titer of 6 x 106 infectious virus particles/mL. This virus forms plaques on monolayers of the monkey kidney cell line, CV-1. Following completion of a 10-fold dilution series, the virologist plates 100 microliters of each sample in duplicate.

Required:
What experimental results did he MOST LIKELY observe in the duplicate plates corresponding to his 1:10000 dilution?

Answers

Answer 1

Answer:

61 for Plate 1 ,

59 for Plate 2

Explanation:

Given that the original stock has a concentration of  6 * 10^6 infectious virus particles /mL

and it was diluted by 1:10000

New concentration = ( 6 * 10^6 ) / 10000 = 600 infectious virus particles/ mL

Also given that the virologist plates 100 mL of each sample

therefore; 0.1 mL has a count of 60.

The experimental results that he is most likely to observe in the duplicate plates will be ;

61 for (Plate 1) ,

59 for (Plate 2)


Related Questions

What are possible effects warming the atmosphere?​

Answers

Higher temperatures are worsening many types of disasters, including storms, heat waves, floods, and droughts. A warmer climate creates an atmosphere that can collect, retain, and drop more water, changing weather patterns in such a way that wet areas become wetter and dry areas drier.

Which of the following are adaptations present in plants or animals that live in the understory level of the rainforests? (Choose all that apply.)
a. large eyes to see in the darkness
b. broad leaves to catch available light
c. vines and structures to climb towards sunlight
d. thick, waxy leaves

Answers

Answer:

large eyes to see in the darkness

b. broad leaves to catch available light

c. vines and structures to climb towards sunlight

Explanation:

This because Adaptation refers to the ability of plants or animals to adapt, survive and reproduce in it's environment through its developed structural which help it to do so. Plants in the rainforest enjoy abundance of rain and there are thick forest of trees and shrubs. Therefore animals there normally have broad eyes to see in the darkness because the trees form shade easily. The plants normally have broad leaves to receive sunlight

Answer:

- broad leaves to catch available light

- vines and structures to climb towards sunlight

- large eyes to see in the darkness

Explanation:

Organisms that live in rainforests have adaptations that enable them to live in the different conditions that exist in each layer.

what are the 5 main important functions of soil​

Answers

Answer:

Six key soil functions are: Soil acts as an anchor for plant roots. It provides a hospitable place for a plant to live in while storing and supplying nutrients to plants. Soil also functions by maintaining the quantity and quality of air by allowing CO

Explanation:

Fonseca is taking a test. She has to write about adaptations of fish in the coral reef. What should she write?
A.
that some fish in the coral reef are blind due to the absence of sunlight
B.
that some fish in the coral reef survive on marine detritus
C.
that some fish in the coral reef are colorless to protect against prey
D.
that some fish in the coral reef have flat bodies to perform sharp turns

Answers

Answer:

that some fish in the coral reef have flat bodies to perform sharp turns

Describe how heat will flow
between Part A and Part B
when the tap is opened. Draw
an arrow to illustrate the
direction of heat flow.

Answers

Answer:

Explanation:

When you bring two objects of different temperature together, energy will always be transferred from the hotter to the cooler object. The objects will exchange thermal energy, until thermal equilibrium is reached, i.e. until their temperatures are equal. We say that heat flows from the hotter to the cooler object.


If humans are really intelligent and not simply manipulated by their genes like
any other animal, why can't they do anything about overpopulation?

Answers

Answer:

Explanation:

What about war? Why can't we do anything about war?

How about our needs and wants beyond shelter and food?

Of all the things plaguing the world, I think over population is one of the things we can do something about. You are right. But needs and wants play a huge role in why we can't seem to do anything right.

Of course on thinking it over, we have problems that have their roots in overpopulation like pollution.

Why does Matt call his dog?

A)He wants to give his dog a hug.
B) He wants to take his dog for a walk.
C) He wants to give his dinner to the dog.
D) He wants his dog to go into the kitchen.

Answers

The correct answer is C, he wants to give his dinner to the dog.

what organelle directs heredity

Answers

Answer:

The nucleus

Explanation:

The nucleus contains chromosomes that contain genes

IM TIMED PLS HELP

How does deforestation impact greenhouse gases in the atmosphere the most?

A.
Deforestation increases the build-up of greenhouse gases because the removed trees would have consumed carbon dioxide.

B.
Deforestation removes trees, which shelter organisms that release gases that purify the air.

C.
Deforestation decreases the amount of greenhouse gases because trees produce many of them.

Answers

Answer:

Deforestation increases the build-up of greenhouse gases because the removed trees would have consumed carbon dioxide.

Explanation:

Answer:

A.  Deforestation increases the build-up of greenhouse gases because the removed trees would have consumed carbon dioxide.

Explanation:

Burning fossil fuels, in combination with the destruction of carbon sinks due to deforestation and other activities, has contributed to more and more carbon dioxide building up in the atmosphere – more than can be absorbed from existing carbon sinks such as forests.

In ecosystems, plants transform light energy from the Sun into chemical energy when they make sugar. This sugar can then be consumed by other organisms to be used as building blocks for other molecules, such as proteins and fats, or it can be transformed into other forms of energy, such as kinetic energy, when the organism moves. Which of the following statements is supported by the above information? A. Energy can change forms, but only one kind of matter exists. B. Matter can change forms, but only one kind of energy exists. C. Matter and energy can change forms and locations in ecosystems. D. Matter and energy must always remain in the same form and location.

Answers

Answer:

The answer is C. Matter and energy can change forms and locations in ecosystems.

Explanation:

The answer is the matter

The diagram shows which structure of the nervous system?
Dendrite
Axon
Myelin sheath
the meninges
a vertebra
a neuron
the cerebellum

Answers

Answer:

C. a neuron

Explanation:

took the cumulative test 100% edge 2021

Which of the following statements best describes what happens during exercise?
(Please explain why each of the incorrect statements are wrong. Thank you!)

A.
Blood vessels in the muscles dilate to increase blood flow.

B.
Blood vessels in the heart dilate to increase blood pressure.

C.
Blood vessels in the organs dilate to increase blood oxygen.

D.
Blood vessels in the lungs dilate to increase blood volume.

Answers

the answer would be D

An event that causes changes to the living or nonliving parts of an ecosystem is called a ________________?

succession

disturbance

species

none of the above

Answers

Answer:

Explanation:

2nd one

disturbance would be the correct answer

What is meant by "nutrient limitation

Answers

Answer:

meaningful additions of an essential element

Explanation:

Nutrient limitation occurs when meaningful additions of an essential element in additions of an essential element in biologically available forms cause an increase in the rate of a biological process (such as primary productivity) and/or in the size of an important ecosystem compartment (such as bi ) biomass).

Nutrient limitation occurs when meaningful additions of an essential element in additions of an essential element in biologically available forms cause an increase in the rate of a biological process (such as primary productivity) and/or in the size of an important ecosystem compartment (such as bi ) biomass).

Which would indicate the water in a lake is not safe for drinking? A. many varieties of fish in the lake B.high dissolved oxygen levels in the lake C. cold water temperature of the lake D. high turbidity levels in the lake​

Answers

D high turbidity, that’s muddy looking water

The water in a lake is not safe for drinking, high turbidity levels in the lake​ Coliform bacteria occur innately in soil and in the intestines of humans and other organism.

What is dissolved oxygen in water?

Dissolved oxygen (DO) is the quantity of oxygen that is current in water.

Water bodies obtain oxygen from the atmosphere and from aquatic plants.

Rushing water, such as that of a fast moving stream, dissolves more oxygen than the still water of a pond or lake

Thus, option "D" is correct, high turbidity levels in the lake​.

To learn more about turbidity  click here:

https://brainly.com/question/26612834

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The role of the 23S rRNA of the large ribosomal subunit of E. coli during protein synthesis is: Question 50 options: helps to form the A site within the ribosome contributes to the process of translocation during the elongation stage of protein synthesis none of these choices is correct actively forms a peptide bond by acting as a ribozyme aid in initiation of protein synthesis

Answers

Answer:

Actively forms a peptide bond by acting as a ribozyme aid in initiation of protein synthesis.

Explanation:

The role of the 23S rRNA of the large ribosomal subunit of E. coli during protein synthesis is the formation of a peptide bond by acting as a ribozyme aid in initiation of protein synthesis. 23S rRNA has an important function of peptidyl transferase. Both peptidyl-tRNA and aminoacyl-tRNA are necessary for synthesis of protein and transpeptidation response. The 23S rRNA positions have a significant function in the binding of tRNA in P site of the large ribosomal subunit.

a mass extinctions is

Answers

Answer:

Mass extinction is when biodiversity or species in a living habitat decreases to about three-fourths of its original number or to none. Such an event is identified by a sharp change in the diversity and abundance of multicellular organisms. It occurs when the rate of extinction increases with respect to the rate of speciation. Mass extinctions are usually associated with organisms that are macroscopic rather than microscopic. Examples of mass extinctions are Permian extinction of marine species, and Cretaceous extinction of various species, including dinosaurs.

Manipulate the settings to model the scenario. Observe what happens to the
observations in the Lab Guide,
About laboratory work
Stream Slope
Gentle
Amount of Rainfall
None
Surrounding Terrain
Plain
Mountains
Check
Pause
5 of 10

Answers

Answer:

subject?

Explanation:

Only you can complete this question because only you have access to the necessary modeling program.

I’m not sure but do anybody know this ‍♀️

Answers

deletion is the correct choice

Give an example of homologous and analogous structures

Answers

homologous:  the forelimbs of vertebrates

 analogous the wings of an insect, bird, and bat

Because of global climate change, arctic landscapes and ecosystems are changing rapidly and perceptibly, as the residents of Newtok, Alaska, can attest. With the land upon which they have built their homes slowly melting and sinking, they have appealed to the state and federal governments for assistance in helping them cover the costs of moving their town to a different location. Ironically,
A) the land upon which the Alaskan state government buildings are located is also melting.
B) the residents of Newtok have discovered oil on their land, making their appeal for funds less convincing.
C) a senator from Alaska has a vacation home in Newtok, Alaska, and so is personally committed to the predicament of the town.
D) decades ago, the U.S. government mandated that they and other Alaskan natives abandon a nomadic life based on hunting and fishing for sedentism.
E) the economic activity of the town of Newtok is extremely polluting and thus a big contributor to the environmental changes that have turned its residents into the first climate change refugees in the United States.

Answers

Answer:

The correct answer is D)

Decades ago, the U.S. government mandated that they and other Alaskan natives abandon a nomadic life based on hunting and fishing for sedentism.

Explanation:

Sedentism is simply the practice of settling in one geographical location for a long time.

Many governments encourage this to ensure that agricultural systems become developed and more sustainable as was the case in Alaska.

Other governments in some part of the world are also in support albeit for a different reason such as security of lives and property.

Cheers

why are minerals important for our body? describe the 2 groups of minerals

Answers

Minerals are needed for the proper composition of body fluids, including blood, and for the proper composition of tissues, bone, teeth, muscles and nerves. Minerals also play a significant role in maintaining healthy nerve function, the regulation of muscle tone, and supporting a healthy cardiovascular system. There are two kinds of minerals: macrominerals and trace minerals.

Explanation:

.

Which of the following describes a portion of the water cycle that is not driven by
gravity? O
(1 Point)
B) Precipitation of water that has condensed from clouds
D)Water seeping into the ground to enter an aquifer
C)Water flowing in a river toward the ocean
A) Evaporation of water from the ocean to form clouds

Answers

The correct answer is A. Evaporation of ocean water to form clouds.

Explanation

The water cycle is a series of physical-chemical phenomena in which water goes through three states (liquid, solid and gaseous). In this process, the water in a liquid state is evaporated becoming in a gaseous state, and rising towards the sky (against the force of gravity) forming clouds. Once in the clouds, the water condenses, going from a gaseous to a liquid state. After this process, the water precipitates by the action of gravity towards the earth where a process such as the filtration of water in the soil occurs, forming aquifers, the accumulation of water in the rivers that flow into the sea, the freezing of water on the snowy peaks of the mountains, among others. According to the previous explanation, the correct answer is A. Evaporation of ocean water to form clouds.

Which human organ system’s function is most similar to the function of a cell wall in plant cells?

Answers

Answer:

The cell wall, vacuole, and chloroplast are found in plant cells not human body.

Answer:

the respiratory system!

Explanation:

i just took a quiz and this was one of the questions, i got it right

Select the correct answer.
What is true about the theory of punctuated equilibrium?

A. It relies on the discovery of "missing links."
B. It explains the absence of transition in fossils.
C. It portrays evolutionary change as a smooth curve.

Answers

Answer:

C. It portrays evolutionary change as a smooth curve.

I need the answer pls don’t tell the wrong answer and this is science

Answers

I believe it is Weather

what hereditary factors affect ones growths​

Answers

Your sex given at birth is a major factor that can determine your growth
These include things such as genetics, parenting, experiences, friends, family, education, and relationships. By understanding the role that these factors play, researchers are better able to identify how such influences contribute to development.


The graphs here show the distribution of beak depths for the ground
finches on Daphne Major, an island in the Galapagos. The bird beaks were
measured over a multi-year study, including a time period before and after
a drought. The drought reduced the supply of seeds and left most of the
seeds as thick-walled nuts that survived the drought. Analyze the data and
select the claim about natural selection and genetic diversity that is
supported by the data.

Natural selection tends to reduce genetic variation, as there was a smaller distribution
of beak depths, but it tends to select for the most fit phenotypes, as there was an
increase in average beak depth after the drought.

Natural selection tends to increase genetic variation, as there were more ground
finches after the drought, and it tends to select for the most fit phenotypes, as there
was an increase in average beak depth after the drought.

Natural selection tends to reduce genetic variation, as there was a smaller distribution
of beak depths, but it tends to select against the most fit phenotypes, as there was an
increase in average beak depth after the drought.

Natural selection tends to increase genetic variation, as the average beak size
increased after the drought, but it tends to select against the most fit phenotypes, as
there was a decrease in population size for the finches after the drought

Answers

Answer: (A) Natural selection tend to reduce genetic variation as there was a smaller distribution of beak depths, but it tends to select for the most fit phenotypes, as there was an increase in average beak depth after the drought.

Explanation: The picture below shows proof if needed. On USATP

Natural selection tend to reduce genetic variation as a smaller allocation of beak depths, but it tends to select for the most fit phenotypes, as an increase in average beak depth after the drought.

How does natural selection affect genetic variation?

Genetic variations that vary gene and protein role.

If a trait is favourable and helps the someone survive and breed, the genetic variation is more likely to be passed to the next generation.

Thus, option "A" is correct by selecting for or against a specific gene.

To learn more about  Natural selection click here:

https://brainly.com/question/9830102

What percentage of the global population lives in the United States?

Answers

Answer: 4.25%

Explanation:

As at Friday, May 28, 2021, the population of the United States of America is about 332,754,370. The population of the world is about 7,868,904,000.

Therefore, the percentage of the global population lives in the United States is about 4.25% that's 332,754,370/7,868,904,000 × 100%.

What is the major issue that we are faced with as discussed in this video kiss the ground What is the proposed solution? plssss

Answers

Save our soil in hopes the soil might just save us.” This is the central statement of Kiss the Ground I think the major issue is that we need to save our soil because our soul is dying out.
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