Based on your understanding of nucleic acids, what type of bonds form between the CRISPR/guide RNA molecule and the target DNA

Answers

Answer 1

Answer:

The single guide RNA forms hydrogen bonds with DNA, while Cas9 hydrolyzes phosphodiester bonds

Explanation:

The base pairing between nucleic acid strands (either DNA or RNA) is through hydrogen bonds between nucleotide bases. In DNA, Adenine always forms two hydrogen bonds with Thymine, while Guanine always forms three hydrogen bonds only with Cytosine. Moreover, adjacent nucleotides in the same strand are covalently linked by phosphodiester bonds (i.e., covalent bonds between the 5' phosphate group of one nucleotide and the 3'-OH group of another). The CRISPR/Cas9 genome editing systems make use of single-guide RNAs (sgRNAs) that interact with DNA through hydrogen bonds. These sgRNAs have perfect complementarity to the target DNAs in order to bind them. On the other hand, Cas9 is an enzyme that hydrolyzes phosphodiester bonds in both DNA strands very precisely and accurately by using a sgRNA complementary to a specific DNA sequence.


Related Questions

Dichotomous key practice: a key to insects
Please help !!!!!!!!!!

Answers

Answer:

Answer to 1 is a grasshopper (order Orthoptera)

Answer to 2 is a dragonfly (order Odonata)

Answer to 3 is a fly (order Diptera)

Answer to 4 is a bee (order Hymenoptera)

Answer to 5 is lady bug (order Coleoptera)

Answer to 6 is an aphid (order Hemiptera)

Answer to 7 is a butterfly (order Lepidoptera)

Hope this helped you.

Explanation:

Answer to 1: is a grasshopper (order Orthoptera). Orthopterans have a cylindrical body, with elongated hind legs and musculature adapted for jumping.

Answer to 2: is a dragonfly (order Odonata). Thise groups have minute antennae, two pairs of transparent membranous wings with many small veins, a long slender abdomen, extremely large eyes, an aquatic larval stage (nymph) with posterior tracheal gills.

What is dichotomous key?

A dichotomous key is refer to as an important scientific tool which is used to identify different organisms, based the organism's observable traits. Dichotomous keys comprises of a series of statements with two choices in each step that will lead users to the correct identification.

Answer to 3: is a fly (order Diptera). This group are true flies, are a large and diverse group that includes midges, mosquitoes, and all manner of flies. Diptera literal means "two wings," the unifying characteristic of this group.

Answer to 4: is a bee (order Hymenoptera). This group have small to medium-sized insects, usually with four membranous wings and a narrow waist which sets off the abdomen from the thorax, or middle region of the body.

Answer to 5: is lady bug (order Coleoptera). This group have sheath-winged which is the hardened forewings (elytra) six legs and antennae.

Answer to 6 is an aphid (order Hemiptera). This group is called as true bugs and have forewings that are hardened at the base and membranous at the tips.

Answer to 7: is a butterfly (order Lepidoptera). This group are typical insects, in which they have 4 wings, 6 legs, 2 antennae.  The body divided into 3 sections such as a head, thorax and abdomen. The leg and wings are attached to the thorax.

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What are the adaptive features of animal in rainforest

Answers

Answer:

Many animals have adapted to the unique conditions of the tropical rainforests. The sloth uses camouflage and moves very slowly to make it difficult for predators to spot. The spider monkey has long, strong limbs to help it to climb through the rainforest trees.

Hope it heps

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Before nitrogen gas can be used by living organisms it must be converted into ammonium. This important step of the nitrogen cycle is known as
1. ammonification
2. denitrification
3. ozonation
4. nitrification​

Answers

Answer:

dont understand, so sorry!! once again, so sorry!!!!

according to the article why is it important for pikas to find cool habitats

Answers

their normal body temperature is high, and this puts them at risk of overheating when they’re active in warm environments

There are marked differences in the type of organisms found at four different locations at the same tidal height along ta rocky shore. What might account for this

Answers

Answer and Explanation:

Many elements can be responsible for this, among them, we can mention the presence and absence of predators in the different places on the rocky coast. This is because predators can influence not only the number of organisms found, but also the types of organisms.

Another element that may be the cause of this variability is the availability of resources necessary for the life of these organisms, in addition, the impact of the waves on the rocks, can cause the variability of the organisms, since some may not be able to resist the impact of the waves.

What is the answer answer to number one will mark brainlist

Answers

Answer:

GGATTTGGCCCTATA

Explanation:

A pairs with T and C pairs with G. This is called the complementary pairing.

So answer is GGATTTGGCCCTATA

If plants are absorbing and using blue and red light, what must be happening to other colours of light, like green and yellow?

Answers

Answer:

If plants are absorbing blue and red light other colors such as green and yellow are being reflected. For example, a green plant absorbs all colors of light and reflects green, which gives it its green color.

Plants absorb and use blue and red lights during the process of photosynthesis, and the electrons get excited which carry out other pathways. While the lights like green and yellow which are not absorbed are reflected which are visible to our eyes.

What is light absorption in plants?

Plants contain a pigment in the chloroplast which is known as chlorophyll. This pigment is present in all the green plants, it is responsible for trapping the light energy which comes from the Sun or artificial source.

Chlorophyll absorbs the light of different wavelengths in the visible range of light. This is known as absorption spectrum of the plant. Rest of the other wavelengths of light are reflected from the plant body which are visible to the eyes.

The wavelengths of red and blue lights show maximum rate of photosynthesis in comparison to other lights.

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what darwin never knew essay

Answers

Answer:

What Darwin Never Knew”, is a stunning time line that details the theory of evolution formed by Charles Darwin, and the recent advancements made that answers some of the questions he simply could not. Darwin 's theory explained why today there are 9,000 kinds of birds, 350,000 kinds of beetles, 28,000 kinds of fish, and at least 2 million kinds of living species and counting. Darwin figured out that all species are connected, and he also realized that species evolved and adapted, but he did not know how. Darwin made a five year voyage on The HMS Beagle that would change his life, and all life as a matter of fact, forever.

Question 5 Multiple Choice Worth 5 points)
(01.01 LC)
Which is a long-term benefit of fitness?
High blood pressure
Bone health
Arthritis
Depression

Answers

B. Bone health is the anser

Answer:

B. Bone health

Explanation:

Engaging in specific exercises helps us build and maintain stronger bones, allowing us to continue to do daily activities as we age.

The passage indicates that compared with other functions of coloration in plants, camouflage in plants has A) provided scientists with a deeper understanding of potential food sources. B) made use of a wider variety of distinctive shades of colors. C) proved to be a less effective defense against predators. D) been the subject of a smaller number of scientific investigations.

Answers

Question passage: This PASSAGE is adapted from “Rare Woodland Plant Uses‘Cryptic Coloration’ to Hide from Predators.” ©2009 byAmerican Journal of Botany, Inc.It is well known that some animal species usecamouflage to hide from predators. Individuals thatare able to blend in to their surroundings and avoidbeing eaten are able to survive longer, reproduce, andthus increase their fitness (pass along their genes tothe next generation) compared to those who standout more. This may seem like a good strategy, andfairly common in the animal kingdom, but who everheard of a plant doing the same thing?In plants, the use of coloration or pigmentation asa vital component of acquiring food (e.g.,photosynthesis) or as a means of attractingpollinators (e.g., flowers) has been well studied.However, variation in pigmentation as a means ofescaping predation has received little attention.Matthew Klooster from Harvard University andcolleagues empirically investigated whether the driedbracts (specialized leaves) on a rare woodland plant,Monotropsis odorata, might serve a similar purposeas the stripes on a tiger or the grey coloration of thewings of the peppered moth: namely, to hide.

The PASSAGE indicates that compared with other functions of coloration in plants, camouflage in plants has A) provided scientists with a deeper understanding of potential food sources. B) made use of a wider variety of distinctive shades of colors. C) proved to be a less effective defense against predators. D) been the subject of a smaller number of scientific investigations.

Answer: The correct option is C(proved to be a less effective defense against predators).

Explanation:

Adaptation is a means by which plants or animals modify their forms, structures, functions and behaviour in order to escape being harmed and survive in their environment. The special adaptations of organisms in a habitat enables them to live there successfully and reproduce. There are 3 types of adaptation which include:

--> structural adaptation,

--> adaptive coloration and

--> behavioural adaptations.

From the passage, the plant under study shows ADAPTIVE COLOURATION. Colour and colour pattern plays an important role in adaptations of plants. These occur due to the presence of pigments in cells of plants. Common pigments responsible for coloration in plants are:

--> chlorophyll: this gives green colour to plants that has chloroplasts in their cells.

--> Carotene and xanthophyll: this gives yellow to orange colour to plants that has chromoplasts in their cells.

From the passage, the use of coloration or pigmentation in the plant as a vital component of acquiring food (e.g.,photosynthesis) or as a means of attracting pollinators (e.g., flowers) has been well studied but it's use to escape from predation has received little attention. Therefore camouflage in plants has proved to be a less effective defense against predators.

King Penguins and Fur Seals King Penguins and Fur Seals are both designed for swimming, and thus are very clumsy on land. With that in mind, answer these questions: Why do the penguins come onto land at all

Answers

Answer:

The correct answer is - chicks will not be able to survive in water.

Explanation:

Penguins spend the majority of their life in the water and for mating and moult they move to land and also lay their eggs on land. The wings or flippers of the chicks of these penguins are not as efficient as the wings of the adult penguins.

This makes it tough to survive on the waters for the chicks of penguins. Therefore they move to land even they are very clumsy on the land. The mating, molt and raising the chicks to few weeks are the prime reason.

Match these items.


1. an atom that has gained or lost electrons

____base

2. produces OH - ions when added to water

____acid

3. produces H+ ions when added to water

____ion

Answers

1. Ion
2. Base
3. Acid

List three different types of posttranslational modifications that may happen to a protein and the significance of each in the context of protein function.

Answers

Explanation:

These modifications include phosphorylation, glycosylation, ubiquitination, nitrosylation, methylation, acetylation, lipidation and proteolysis and influence almost all aspects of normal cell biology and pathogenesis.

BSC 1005

Do you notice any notice any trends or can you make any generalizations about where certain types of liquids fall on the pH scale?

Answers

Answer:

Basic liquid sea water and ammonia solution. Acidic liquid lemon juice and gastric juice. Neutral liquid water and blood.

Explanation:

Liquid pH

Lemon juice 2

Gastric juice 1.5-3.5

Sea water 8

Ammonia solution 11

Water 7

Human Blood 7.4

solutions to stop dams

Answers

Answer:

break it down I guess

Explanation:

its a simple question and I'm pretty sure you made a typo oh well

What is the process of a species producing more offspring than can survive called?

Answers

Answer:

because you keep producing more and more

Explanation:

Natural selection involves heritable variation (caused by mutations in the genetic material), overproduction of offspring, preferential survival of individuals having variations favorable for the environment, and reproduction by survivors.

A blood bank refused to accept blood from a potential donor who had just had his teeth cleaned by a dental hygienist. Why did they refuse the blood

Answers

Answer:

High amounts of fluoride

Explanation:

Teeth cleaning agents can contaminate one's blood with fluoride, something that it is only healthy to accumulate a small amount of.

A blood bank would refuse the blood of the potential donor because the cleaning agents of teeth significantly accumulate heavy metals in the blood of an individual.

Why teeth are required to be cleaned by a dental hygienist?

Teeth are required to be cleaned regularly on their own. But if you are facing some problems like tooth decay you must have to consult with a dental hygienist in order to ensure the health of teeth and gums.

According to the context of this question, a dental hygienist uses a variety of cleaning agents while cleaning the teeth of a patient. Due to this, the accumulation of concentration of heavy metals like fluoride and lead is increased in the blood directly or indirectly.

Apart from this, this person also has genetic inheritance of tooth decay and other diseases. So, the blood bank refused to accept blood from a potential donor.

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PLEASE HURRY, WILL GIVE BRAINLY TO CORRECT ANSWER!

which of the following makes it difficult to reach and explore the outer planets?
1. the surfaces are too icy and slippery to land on.
2. they contain a lot of helium in their atmospheres.
3. massive storms with fast winds cover the surfaces.
4. none of the above.
5. both 1 and 2
6. both 2 and 3.

Answers

Answer:

6 is the most prefarable

Explanation:

2 parents one with AB blood type and one with b type can they produce a child with A blood type

Answers

Answer:

No they can not produce a child with a blood type

Chemical energy in the form of food is changed into what?

Answers

Food is the most important source of chemical energy for living things. The chemical energy in food is converted, or changed, by the body into moving mechanical energy and heat energy. People have invented ways to use the chemical energy of substances to power machines.

A car accelerates from 4 meters/seconds to 16 meters/second in 4 seconds.The car’s acceleration is blank meter/second2

Answers

Answer:

3m/s²

Explanation:

a = v - u/t

Where;

a = acceleration (m/s²)

v = final velocity (m/s)

u = initial velocity (m/s)

t = time (s)

According to this question, a car is said to accelerate from 4 m/s to 16m/s in 4 seconds. This means that;

v = 16m/s

u = 4m/s

t = 4s

a = 16 - 4/4

a = 12/4

a = 3m/s²

2. A scientist wants to clone a molecule bearing UniProtKB accession number P43657. How will he come to
know about the following?
I. Exact genomic location of the molecule under investigation
II. Coding and noncoding segments of the molecule
III. Untranslated regions of the molecule

Answers

Answer:

II. Coding and noncoding segments of the molecule

Explanation:

mark brainliest

definition of human rights​

Answers

A human rights violation is the disallowance of the freedom of thought and movement to which all humans legally have a right. While individuals can violate these rights, the leadership or government of civilization most often belittles marginalized person

Describe the three factors that affect the rate of photosynthesis.

Answers

Answer:

light intensity, carbon dioxide, and temperature

Explanation:

light Intensity because it depends how much sunlight the plants absorb

carbon dioxide because plants need a lot of CO2 to produce photosynthesis

temperature because it shouldn't be too cold or too hot, it should be room temperature

hope that helped :)

Please answer only if you know this is a final 

Answers

Answer:

Explanation:

The symbiotic relationship between a flower and the insect that feeds on its nectar is an example of a. mutualism because the flower provides the insect with food and the insect pollinates the flower.

Red-green color blindness is a human X-linked recessive disorder. Jill has normal color vision, but her father is color blind. Jill marries Tom, who also has normal color vision. Jill and Tom have a daughter who has Turner syndrome and is color blind. a. How did the daughter inherit color blindness

Answers

Answer:

From her mother's (Jill's) father.

Explanation:

A substance that yields a cation plus the hydroxyl ion in water is a(n)_____.
A.Salt
B.Acid
C.Base

Answers

Question:

A substance that yields a cation plus the hydroxyl ion in water is a(n)_____.

Answer:

C.Base

Did you know that a base is a substance that yields a cation plus the hydroxyl in ion water. A hydroxyl ion is a negatively charged molecule made up of one oxygen bonded to one hydrogen.

Hence, a base is a substance that accepts hydrogen ions. A base is also a compound that produces hydroxide ions (OH+) in solutions.

A base forms OH- ions in solution. It has a pH greater than 7.

Mind you all people that the number of hydroxide ions in solution is greater than the number of hydronium ion. Thats all

_________

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1. Imagine that you collected a worker ant from an ant colony, and you found that it was positive for Wolbachia. Would you expect the ant queen from that colony to also be positive for Wolbachia

Answers

Answer:

Yes.

Explanation:

Yes, the ant queen from that colony to also be positive for Wolbachia because Wolbachia also cause infection in the ant queen. This bacteria lives in the cell of their host and cause infections. This bacteria specie is responsible for the reproductive and developmental changes in the invertebrates so we can conclude that Wolbachia also affect the ant queen of that colony.

Coal burning power plants are required to measure the waste gases they produce.

What data is most important for the measurement technology to report?

A. the time of day the gases are released

B. the amount and type of gases produced

C. the temperature and color of the waste gases

D. the movement of gases through the power plant

Answers

Answer:

I think b is the correct answer of the question

BIOLOGY HELPS PLEASE

Answers

Answer:

b

Explanation:

If the small-beaked birds can't eat, they will die

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