Fire and Mello conducted an experiment where they added no gene X RNA (b), anti-sense gene X RNA (c) or double stranded gene X RNA (d) to C. elegans embryos. They then did a hybridization experiment examining gene X mRNA levels in the embryo. Square (a) is a no probe control. How does this act as a control

Answers

Answer 1

Answer:

The correct answer is - It shows that an RNA probe is necessary to get a signal.

Explanation:

A probe is a specific single-stranded sequence of DNA or RNA that recognizes its complementary sequence in a particular sample genome for synthesis. The RNA probe is placed into close contact with the given sample under a specific environment that allows the RNA probe sequence to hybridize with its complementary sequence.

In this case, square a is the condition where no RNA probe is persent whereas all three other conditions have some sort of RNA probe, however, in C there is no Gene X probe but their probe present. To get a signal in hybridization RNA probe is required and that can be used as control as it can be used to compare if a signal is received or not.


Related Questions

which of the following elements is most likely to be the least reactive?
A. a group of 2 element
B. a group of 17 element
C an element with 1 valence electron
D an element with 8 valence electrons

Please help1!1

Answers

Answer:

D

Explanation:

8 valence electrons means it is stable, so it will not need to lose or gain electrons

PLS HELP 40 POINTS

Which of the following statements correctly compares sex-linked disorders between males and females?

Sex-linked disorders affect females more often than males because females have no X chromosomes.
Sex-linked disorders affect males more often than females because males have only one X chromosome.
Sex-linked disorders affect females more often than males because females have only one Y chromosome.
Sex-linked disorders affect males more often than females because males have only one Y chromosome.

Answers

Answer:

i have no idea what this means i kinda need more details but ill say it maybe is Sex-linked disorders affect males more often than females because males have only one X chromosome. but im not really sure

Explanation:

13. Which kingdom is known as a decomposer? A. Fungi B. Plantae C. Protista D. Animalia Dentisthenia​

Answers

Answer:

fungi

Explanation:

.......................

Answer:

I think it's D

Explanation:

Have a Nice Day

5 The systolic blood pressure reading of 140-159 the systolic blood pressure reading of 140 - 159 is what stage of Hypertension?
A Stage 2
B Stage 1
C Stage 3
D High Normal

Answers

Answer:

B. Stage 1

Explanation:

Systolic blood pressure is less than 120 mm Hg and Diastolic less than 80 mm Hg so it is considered as normal. Pre-Hypertension occurs when the Systolic blood pressure is 120 - 139 and Diastolic pressure is 80 - 89. Stage 1 Hypertension is considered when the Systolic blood pressure is 140 - 159 and Diastolic blood pressure is 90- 99. If the Systolic blood pressure is greater than 160 it is considered as Stage 2 Hypertension.

What is food hygeine​

Answers

Answer:

Food hygiene is an essential aspect of food safety. It refers to the processes that directly involve food – including storage, preparation, and cooking. Good practices in these areas ensure that people receive food that's safe and as described.

The budding of clathrin-coated vesicles from eukaryotic plasma membrane fragments can be observed when adaptations, clathrin, and dynamin-GTP are added to the membrane preparation. What would you observe if you omitted (A) adaptations, (B) clathrin, or (C) dynamin

Answers

Answer:

Explanation:

Adaptins:

Adaptor proteins, which attach the clathrin to the membrane, are required for clathrin-coated vesicles to form. Clathrin cages form in solution at strong clathrin concentrations and under the right ionic environments and parameters, but they are empty hollow shells without membranes or other proteins. The data for clathrin baskets are included in the clathrin molecules themselves, as shown by the self-assembly of clathrin into baskets. Clathrin coat cannot form or build up without adjusting to the membrane it is attached to.

Clathrin:

Adaptins bind and act synergistically to specific receptors without clathrin, but no vesicles or clathrin are formed. There exists coated pits formations, but no clathrin coat.

Dynamin:

Clathrin-coated pits can develop and progress toward vesicle formation in the exclusion of dynamin, but the final step, known as membrane fusion, is inhibited. Consequently, in the exclusion of dynamin, strongly invaginated covered pits will be noticed. As a result, vesicles are unable to pinch the membrane.

Suppose plasma membrane fragments were prokaryotic cells, there would be no endocytosis and hence no adaptin or coatings. Endocytosis is not performed by prokaryotic cells. There are no receptors with adequate cytosolic tails in a prokaryotic cell that might facilitate the binding to adaptor proteins. As a result. no clathrin-coated vesicles will develop.

Which organism is most abundant in the Terrestrial food chain

Answers

The primary producers

An electric current is generated when a coil of wire passes through a magnetic
field. A device for detecting this current is a
1 generator
2 turbine
3 calorimeter
4 galvanometer​

Answers

Answer: Galvanometer

Please explain how shade-adapted plants tend to differ from sun-adapted plants, using the terms: specific leaf area, dark respiration, root mass fraction, leaf lifespan.

Answers

Answer:

Thickness and number of chlorophyll.

Explanation:

Shade-adapted plants differ from sun-adapted plants due to many characteristics such as thickness and number of chlorophyll. Sun leaves are more in thickness than shade leaves because they develop longer palisade cells. Shade leaves generally have more chlorophyll as compared to sun-adapted plants. Specific leaf area and root mass fraction of Shade-adapted plants are higher as compared sun-adapted plants due to large number of chlorophyll to harvest more sunlight for photosynthesis. Dark respiration is higher in sun-adapted plants when there is darkness as compared to shade-adapted plants which produce food in no direct sunlight.

Alleles are described as ____________________. homologous chromosomes homologous chromosomes alternate versions of a gene alternate versions of a gene alternate phenotypes alternate phenotypes environmental factors that affect gene expression

Answers

Answer:

The correct answer is - alternate versions of a gene.

Explanation:

In a cell, if there are two copies of each chromosome, these are called homologous chromosomes, one come from the father and the other comes from the mother, which helps in promoting the variety and stability of a species.

Different or alternate versions of genes are possible that can result in two phenotypes for the same trait, these alternate versions called alleles. Alleles can be dominant and recessive.

Which statement correctly identifies the types of macromolecules that are described?

Answers

Answer:

macromolecules are the molecules that we can not see by the eye

Explanation:

What should I do more, that's all I know about macromolecules

Identify the complementary strand of DNA with the sequence: ACC GTA TCG

1.) GTT ACG CTA
2.) ACC GTA TCG
3.) CAA TGC GAT
4.) TGG CAT AGC

Answers

Answer:

4

Explanation:

because it's there opposite letter

Answer:

4 b.c a is equal with t and g is equal to c

cardiovascular anatomy describe the operation of the heart and vessels. true\false​

Answers

Explanation:

true

I hope this helps u

//^

Answer:

susieiwiiuw7wuwiwiwiwususususu

Most food chains end with a top predator. Which of these is the BEST definition of a top predator?

Answers

Answer:

Top predator is a predator at the top of a food chain without natural predators.

Answer:

Hello There!!

Explanation:

A top predator is a predator which is at the top of the food chain and eats other consumers.

hope this helps,have a great day!!

~Pinky~

PLEASE HELP- Which step in developing a
balanced ration involves
determining what feedstuffs are
available in your location?

Answers

Answer:

1

Explanation:

step 1 because it shows locations

ANSWER FOR BRAINLIEST

Look at the image below.

Answers

Y is the best graph represented
the answer will be C. (Y)

1. identify the organelle that regulates cell function and contains the dna

A. Nucleus

B. mitochondria

C. golgi apparatus

D. cell membrane

Answers

Answer:

the organelle that regulates cell function and contains the DNA is a nucleus

All of these children (including the fraternal twin sisters in the front row)
are the biological children of the two parents in the middle of the picture.
Based on everything that you have learned in this unit, explain why these
brothers and sisters do not look exactly alike. Include the processes of cell
division and the rules of genetics in your answer.

Answers

Answer:

Explanation:

Because in the fetus stages of fraternal twins two different eggs get fertilized. Therefore, genetics won't be the same for both twins. But if they were identical twins the fertilized egg was to divide and the two zygotes would've carried the same genetics of each other.

What adaptations do desert animals have that help them survive the hot dry desert?

Answers

Answer:

animals also have to cope i the desert, using adaptations such as being nocturnal or living under ground to survive. camels often live in deserts that are hot and dry during the day, coping with wind-blown sand and cold at night. slit-like nostrils and two rowa of eyelashes to help keep the sand out of their eyes.

In an energy pyramid, the lowest level has: a) less energy than the top level. b) less energy than the second level. c) more energy than the top level. d) the same amount of energy as the second level.​

Answers

Answer:

I believe the answer would be B because if I'm sure the bottom is grain which has higher levels in grain and glucose I believe and that doesn't produce energy . The second level is meat and that has protein which give us strength.

What is thought to have caused the ice ages?

A) Earth's precession
B) Earth's tilt
C) Earth's rotation
D) Earth's magnetic field

Answers

C-earths rotation I believe that’s the answer

Answer:

A) Earth's precession

Explanation:

Earth's precession is the movement of Earth that acts like a cone.

Earth has three movements: rotation, revolution and precession.

(equinoctial precession)

What does the flagella do in a euglenoid?

Answers

Answer:

Euglena move by a flagellum (plural ‚ flagella), which is a long whip-like structure that acts like a little motor. The flagellum is located on the anterior (front) end, and twirls in such a way as to pull the cell through the water. It is attached at an inward pocket called the reservoir.

Ecologists are interested in quantifying different kinds of biodiversity. In below study outlined here, which kind of biodiversity are ecologists studying?

Ecologists establish 20 small areas, called plots, randomly scattered across a California grassland. They count the number of different plant species present in each plot. By noting how many species appear in the plots, the ecologists are able to estimate the number of species in the entire grassland.

a. Genetic diversity
b. Functional diversity
c. Ecosystem diversity
d. Species diversity

Answers

Answer:

d. Species diversity

Explanation:

The species diversity can be measured by:

Species richness: Refers to the number of species are inhabiting a defined area. Species abundance: Describe the distribution of the species abundance.

Species richness is the number of species present in a sample, within a community, habitat or microhabitat, ecosystem, landscape, region or any well-defined spatial unit. It is a metric for measuring biodiversity which simplicity and easy interpretation make it a standard metric.  It is the simplest and straightforward measure of biodiversity. It is just a count of species, with no need for abundance data. In some cases, this can be the cause of criticism, as it ignores information about the species-abundance distribution.    

Match the answer with the statement about promoters and enhancers. Group of answer choices Responsible for regulating translation. [ Choose ] Transcription machinery does not assemble at _______________.

Answers

Answer:

Neither

Chromatin

Explanation:

Both promoters and enhancers are generally regulating transcription. They are not regulating in translation. They are not responsible for regulation in translation.  

Transcription machinery assemble at naked eukaryotic DNA. They do not assemble at DNA chromatin.


What is an element/compound that is
cycled through ecosystem?

Answers

Answer:

Nitrogen, carbon, and phosphorus are just a few

Explanation:

Name the structures of the central nervous system and the peripheral nervous system and describe their functions.

Answers

spinal cord, cranial nerves, white and gray matter, brain, brainstem
.
you can find a very detailed answer on wikipedia which will earn you marks.

Earth's atmosphere is made of different gases that can affect the Earth's Climate in different ways
True/False
Explain in full details.

Answers

Answer:

True. The atmosphere protects life on earth by shielding it from incoming ultraviolet (UV) radiation, keeping the planet warm through insulation, and preventing extremes between day and night temperatures.Greenhouse gases, such as carbon dioxide, methane, nitrous oxide, and certain synthetic chemicals, trap some of the Earth's outgoing energy, thus retaining heat in the atmosphere.

The statement is true.

Effect of atmosphere on climate

Atmosphere of earth contains oxygen which we use for breathing. The gases present in the atmosphere such as ozone protects us from harmful ultraviolet solar radiation.

It creates the pressure without which liquid water couldn't exist on our planet's surface. The greenhouse gases warms our planet and keeps temperatures habitable for our living Earth.

Learn more about climate here:https://brainly.com/question/17922964

wood burning
cooking an egg
making Kool Aid
water boiling
copper plating a nail
melting sand to make glass

are these chemical or physical changes​

Answers

These are physical change examples

PLS HELP ME

Which type of reproduction provides organisms with the following disadvantages? Organisms must grow until mature enough to produce sex cells. Finding a mate uses energy and takes time.

A. Asexual Reproduction

B.Sexual Reproduction​

Answers

Answer:

Sexual Reproduction is the answer

State how a gas produced by photosynthesis is recycled in nature

Answers

Answer:

Oxygen cycle, oxygen the by product of photosynthesis is inhaled by heterotrophs which aids in cellular respiration and other internal processes and then exhales carbon dioxide.

Explanation:

In like manner, carbon dioxide passes from blood to the alveoli and exhaled after.

Other Questions
List the following events in the correct chronological order. Suppose you are a civil engineer, specializing in traffic volume control for the City of Grand Rapids. Your department has been receiving a multitude of complaints about traffic wait times for a certain intersection in the heart of downtown. To see if these claims are valid, you want to monitor the true average wait time at that intersection. Over the course of a few months, you record the average number of minutes a car waits at the intersection between 4:00 PM and 5:00 PM. With a sample size of 9 cars, the average wait time is 6.79 minutes with a standard deviation of 2.2175 minutes. Construct a 99% confidence interval for the true average wait time for a car at the intersection between 4:00 PM and 5:00 PM. i really need help to understand this! The Economist Intelligence Unit rankings of the worlds top education systems,which combine international test results and data such as graduation rates,showed that two contrasting economies topped the league table: Finlandand South Korea. The report concluded that while the amount of spending oneducation is important, it is less influential than an economy having a culturethat is supportive of learning. Education in Finland is 100 per cent state fundedand is rather unorthodox given that students dont start schooling until 7and rarely have tests or homework until their teenage years. In South Korea,education is crucial for success, so there are huge pressures placed on students, who start school aged 3. Testing is regular and rigorous, making after-school private tuition classes extremely popular. According to the report, the top 10 countries were ranked as follows:1. Finland2. South Korea3. Hong Kong4. Japan5. Singapore6. UK7. Netherlands8. New Zealand9. Switzerland10. Canadaa. Explain how scarce resources are allocated in a mixed economy. [4]b. Discuss whether education should be funded by the government. [8] Plot ABC on graph paper with points A(3,3), B(1,1), and C(6,1). Reflect ABC by multiplying the y-coordinates of the vertices by 1. Then use the function (x,y)(x6,y3) to translate the resulting triangle. Name the coordinates of the vertices of the result.Question 3 options:A'(-3,3), B'(-1,1), C'(-1,6)A'(-6,-3), B'(-4,-5), C'(-4,0)A'(3,-3), B'(1,-1), C'(6,-1)A'(-3,-6), B'(-5,-4), C'(0,-4)Plot ABC on graph paper with points A(10,4), B(-1,1), and C(4,2). Reflect ABC by multiplying the x-coordinates of the vertices by 1. Then use the function (x,y)(x5,y+4) to translate the resulting triangle. Name the coordinates of the vertices of the result.Question 4 options:A'(-4,-10), B'(-1,1), C'(-2,-4)A'(-8,15), B'(-5,4), C'(-6,1)A'(-10,4), B'(1,1), C'(-4,2)A'(-15,8), B'(-4,5), C'(-9,6) Does anybody know how to answer this? I need this to answer ASAP. Thank you in advance. Receptor binding motifs such as RGD have highly selective recognition for cell adhesion. Outline a strategy for incorporating a cell adhesive recognition domain to a biomaterial. Address the problems surrounding domain quantity, type, and affinity in your discussion. Which of the following proportional relationships has a higher rate Is -3 a rational number A $20 bill features which of the following on the reverse? Write the fraction 13/25 as a decimal. Show work Which angles are right? Define and describe human trafficking Which of the following would likely happen if a persons lactic acid system had difficulty breaking down glycogen in the muscles?The person would have difficulty running a marathon.The person would have difficulty sprinting in a race.The person would have difficulty swimming across a lake.The person would have difficulty cycling down a hill. Identify which statement is a characteristic of long-run for a firm.A. Time period with at least one fixed elementB. Time period with the least control over constraintsC. Time period of determining quantity and cost that yields the greatest profitD. Time period when constraints are most likely to be variable Help plz..And No links!! I repeat No links!! 2.5x + 1 = 26 help hurryyyyyyyyy!!!!?!?!??!?!?! What is the value of the expression? (14 - 10.25) 2 Name any two professional training needed in profession. What is the equation for density? NO LINKSd = V/md= m + Vd= m x Vd= m/V