How is the absorption of ultraviolet light by DNA and RNA important in the analysis of nucleic acids? A. Nucleic acids absord UV licht maximaly at wavelengths of 247 to 250 m Using this phenomenon one can oftendermine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally a 200 m B. Nucleic acids absorb UV light maximaly at wavelengths of 254 to 260 proteins absorb UV maximally at 200 mm m. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture, since C. Nucleic acids absorb UV light maximalyal Wavelengths of 254 to 280 nm. Using this phenomenon one can hen determine the presence and concentration of nucleic acids in a since proteins absorb UV light maximally at 270 mm D. Nucleic acids absorb UV light maximally at wavelengths of 247 10 250 nm. Using this phenomenon, one can often farmine the presence and concentration of nucleic acids in a mixture, since proteins absorb UV light maximally at 270 nm

Answers

Answer 1

Answer:

B. Nucleic acids absorb UV light maximally at wavelengths of 254 to 260. Proteins absorb UV maximally at 200 nm. Using this phenomenon, one can to determine the presence and concentration of nucleic acids in a mixture.

Explanation:

The concentration of a solution of nucleic acid  can be determined by measuring the absorbance at 260 nm, using a  spectrophotometer. An A260 of 1.0 is equivalent to a concentration of 50 μg/mL for double-stranded DNA, or 40 μg/mL for single-stranded DNA or RNA. If the A280 is also determined, the A260/A280  ratio indicates if there are contaminants present, such as residual  phenol or protein. The A260/A280 ratio should be around 1.8 for pure  DNA and 2.0 for pure RNA preparations.


Related Questions

How are kettle lakes formed

Answers

Answer:

Kettles form when ice detaches from a glacier. Eventually, it becomes wholly or partially buried in sediment and slowly melts, leaving behind a pit.

Answer:

A kettle lake is formed when an inactive block of ice breaks off of a glacier, as time goes on it gets buried sediment and begins to melt, as it melts it leaves behind a pit. Where eventually water will fill it up and the pit will become a body of water.

Why do different colors affect photosynthesis differently

Answers

Answer:

Plants do respond differently to different colors of light being shone on them, because chlorophyll reflects green light and absorbs blue and red (therefore, blue and red are useful for photosynthesis, but green isn't).

Explanation:

what are the two product of the krebs cycle

Answers

it’s NADH and FADH2 !!

What is the definition of biosphere? (1 point)
O the area of the earth that is liquid
O the area of the earth where all living things exist
O the area of the earth that is solid
O the area of the earth covered by water

Answers

Answer:

The area of the earth where all living things exist

The area of the earth where all living things exist, and all ecosystems on Earth that support life are collectively referred to as the biosphere, hence option B is correct.

What is a biosphere?

The biosphere encompasses everything from the deepest tree roots to the shadowy depths of ocean trenches, lush rain forests, lofty mountain summits, and transitional regions like this one where marine and terrestrial ecosystems converge.

The biosphere is the name given to the land, ocean, surface, and atmosphere of the entire planet where life lives.

A population is made up of many species, and a population is made up of a community, an ecosystem, a biome, and a biosphere.

Therefore, the biosphere is the area of the earth where all living things exist, hence option B is correct.

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Gross production is the amount of energy left after cellular respiration. Please select the best answer from the choices provided T or F ​

Answers

Answer:

FALSE

Explanation:

Gross production is the amount of energy left after cellular respiration that is false, as the gross production is the total production and the net production is the amount of energy left after cellular respiration.

What is the significance of the gross production?

Gross production is the total amount of energy produced by an organism through different processes, such as photosynthesis, ingestion, and digestion of food, while after energy is used by the organism for cellular respiration, what is left is called the "net production." Although the gross energy is not released, the net energy is.

Hence, gross production is the amount of energy left after cellular respiration that is false, as the gross production is the total production and the net production is the amount of energy left after cellular respiration.

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What are two activities that might increase your rate of respiration?

Answers

Explanation:

walking

running. As your body is in motion, your oxygen needs are greater. The faster the heartbeat, the more O2 is needed for muscle movement.

That is if your speaking of activity and not cellular respiration...???

True or false statement ? The atmosphere of Venus is dense and mostly made of carbon dioxide?

Answers

Answer:

True. The atmosphere of Venus is the layer of gases surrounding Venus. It is composed primarily of carbon dioxide and is much denser and hotter than that of Earth. The temperature at the surface is 740 K (467 °C, 872 °F), and the pressure is 93 bar (1,350 psi), roughly the pressure found 900 m (3,000 ft) underwater on Earth.

Explanation:

layers of sediment forming at the bottom of the ocean

Answers

Layers of sediment forming on the ocean floor is a form of deposition.

An ABIOTIC factor that can affect an ecosystem is:
A.Virus
B. Locust invasion
C. Mass extinction
D. Hurricane

Answers

Answer:

The answer would be: D. Hurricane

Blood in the middle cerebral artery that has passed the posterior communicating artery can still flow to which region(s) of the cerebrum?​

Answers

Answer: Where would the blood come from if there were a blockage just posterior to the middle cerebral artery on the left? If blood could not get to the middle cerebral artery through the posterior circulation, the blood would flow around the circle of Willis to reach that artery from an anterior vessel.

Explanation:

Weathering is _______.
a.
caused by the movement of materials over rocks
b.
not a natural process
c.
a process whereby rocks are broken down by direct contact with the atmosphere
d.
all of the above

Answers

It is a process where by rocks are broken down by direct contact with the atmosphere

Answer:

C. a process whereby rocks are broken down by direct contact with the atmosphere

Explanation:

EDGE2021

Explain why hunter-gatherer societies were highly mobile.
PLEASE HELP!!!

Answers

Answer:

Hunter and gatherer societies were highly mobile because if the place they they were staying ran out of resources they would have to move. The way that later societies would combat this is by planting farms which could grow back every year and allow them to stay in one place for long periods of time. Hope that helped!

Answer:

Hunter Gatherers rely on the land around them to keep them alive and well, with this being said , if a heard of Buffalo were to move away the village would starve because their main food source would be gone, so they have to “follow their food” and move with nature to stay alive.

Explanation:

100% on endginuity 2021

Many inheritance patterns in traits, especially continuous ones, cannot be accounted for by Mendelian genetics. Why might this be? Select all that apply.
A.
The trait might not be controlled by any genes.
B.
The trait might have multiple genes that control it.
C.
There might not be any alleles that result in the trait.
D.
There might be more than two alleles that result in the trait.
E.
Two alleles might be codominant for the trait.
F.
The trait might not have any codominant alleles.

Answers

Answer:

A, B, C and F

Explanation:

Answer:

I think its D

Explanation:

Answer 25 please thanks

Answers

Answer:

C

Explanation:

which portion of photosynthesis produces 02?

Answers

Explanation:

Answer is chloroplast

What effect do greenhouse gases have on the temperature of our planet?
(plz answer lolz)

Answers

Answer:

Explanation:

The effect of greenhouse gases is that the earth atosphere has been rising consistently for decades and traps extra heat near the surface of Earth.

Which two body systems work together to deliver oxygen through the body?

A. digestive and excretory
B. reproductive and endocrine
C. circulatory and respiratory
D. nervous and digestive

please no gibberish or random letters i need help

Answers

Answer:

C. circulatory and respiratory

Explanation:

The respiratory system is a system that is directly related to breathing and circulatory system is related to blood flow, which contains oxygen.

Answer:

circulatory and respiratory

Explanation:

TOOK THE TEST (:

Again, imagine that your liver, which has a volume of 2 liters, developed from a single embryonic precursor cell with a volume of 1000 μm3, or 1 x 10-12 liters. You previously calculated how many cell division cycles would be required for the precursor cell to produce your mature liver.

What if the control of liver organogenesis were a bit faulty, and the process went exactly 2 cell division cycles beyond this? What then would be the volume of your liver in liters?

I found that the answer to the first question is 41, but I can't figure out the second part.

Answers

Answer:

41 cycles

If cell division went 2 cell division cycle beyond the  precursor; we have 43 cycles

The volume would be = 17.59 Litres

Explanation:

From the given information:

The number of cells in the liver that developed from an embryonic precursor is roughly [tex]\dfrac{2}{10^{-12}}= 2\times10^{12}[/tex]

The number of cell cycles needed to produce [tex]2\times 10^{12[/tex]  cells is:

= [tex]log_2(2\times 10^{12})[/tex]

= 40.86

[tex]\simeq[/tex] 41

Thus, nearly 41 cycles of cell division are required to form the mature liver.

Now;

Suppose 2 extra cell division cycles took place;

The total number of cells would be 2⁴³ = 1.759 × 10¹³; &

The volume would be =  1.759 × 10¹³ × 10⁻¹²

The volume would be = 17.59 Litres

Natural selection disrupts the _______ because one allele of a gene is preferred over another, changing the allele frequencies over time.

Answers

genetic drift

Explanation:

Genetic drift is changes in allele frequencies of a gene pool due to chance or random events. This can occur in large or small populations. Genetic drift causes gene pools of two isolated populations to become dissimilar as some alleles are lost and other are fixed.

need answer ASAP!!
What is the function of the chloroplasts?

Answers

Answer:

Organelles called chloroplasts allow plants to capture the energy of the Sun in energy-

Explanation:

Answer:

Allows the plant to capture energy from the sun.

Explanation:

extra fact, they are organelles.

which component of photosynthesis is a form of energy not matter?
A. water
b. carbon dioxide
c.sunlight
d.oxygen

Answers

Answer:

oxygen

Explanation:

1. Which of the following correctly pairs a structure in a eukaryotic cell with its description?
A nucleus; supports the cell's shape
B. DNA found in cytoplasm
C.
cell membrane; separates a cell from its environment
D. cytoskeleton; consists of all genetic information needed by the cell

Answers

Answer:

B. plants cells have chloroplasts

Explanation:

..

DNA found in cytoplasm is correctly pairs a structure in a eukaryotic cell with its description. Thus, option B is correct.

What are Eukaryotes?

Eukaryotes (or Eukaryota) are a domain grouping all unicellular or multicellular organisms that are characterized by the presence of a nucleus and usually mitochondria in their cells.

He opposes the domains of Eubacteria and Archaea.Eukaryotes gather four great kingdoms of the living world: animals, mushrooms, plants and protists (or protozoa).

Eukaryotes are organisms whose cells have a nucleus enclosed within membranes. All protists, fungi, plants and animals such as yeasts, fungi, Protozoa and Plant cells are examples of eukaryotes. While prokaryote is a unicellular organism that lacks a membrane-bound nucleus, or any other membrane-bound organelle. Bacteria and blue-green algae are examples of prokaryotic cells.

Therefore, DNA found in cytoplasm is correctly pairs a structure in a eukaryotic cell with its description. Thus, option B is correct.

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100 POINTS FOR WHOEVER ACTUALLY DOES IT. I'll post the points in a separate question to avoid cheating. I promise!

For this assignment, conduct some independent research on the raccoon and mountain gorilla. You may do your research online or from library books and magazines.
Part 1: Fill in the table below and then answer the questions.
Trait
How many offspring do they have in a year, usually? What do they eat?
Where are they found in the world?
How many are there in the world?
Are they hunted for meat, skin, fur, or other parts?

Part 2:
Which species is most vulnerable to extinction? Give at least three reasons why.
Which species would be most harmed by environmental change that wiped out one area of its habitat? Why?

Answers

Answer:

Raccoon:

1. Mother raccoons can have between two to five babies in a litter, and they will have just one litter per year.

2. Raccoons are omnivores, meaning they will eat both plants and other animals. This includes fruits, berries, nuts, fish, frogs, mussels, crayfish, insects, turtles, mice, rabbits, muskrats and bird eggs.

3. Raccoons can be found natively throughout most of the US and Canada, as well as in parts of Latin America, from Mexico extending down to the northernmost regions of South America.

4. There are  only 323 to 955 left in the world.

5. They are hunted for their fur and skin.

Mountain Gorilla:

1. Once they have reached an age of approximately 10 years, female mountain gorillas give birth to one baby every 4 years.

2. They eat roots, shoots, fruit, wild celery, and tree bark and pulp.

3.Mountain gorillas are found in two separate locations: the Virunga range of extinct volcanic mountains on the borders of the Democratic Republic of Congo (DRC), Rwanda and Uganda, and in the Bwindi Impenetrable National Park in Uganda.

4. There are only about 1,000 mountain gorillas left.

5. Not only are they hunted for meat, mountain gorillas are also illegally hunted for trophies and live infants.

Part 2:

The mountain gorrila is most vulnerable to extinction. People hunt for mountain gorillas more than they hunt for raccoons. mountain gorillas are on the endangered list. Not only are the mountain gorillas hunted for their meat but they are killed as trophies. Raccoons are rarely out in the daytime making them harder to hunt.

The raccoons would be most harmed by environmental change. Raccoons live in trees and need places to hide in the day time. Gorillas would be able to adapt better considering they dont need to hide in the trees. Raccoons would then be out in the daytime making it easier for them to be caught.

How do the thin membranes of the alveoli in the lungs allow the lung to function?
O A. They provide strength for the lung tissues,
O B. They allow gas exchange to occur more easily.
C. They allow nutrients to be released into the lung,
D. They allow a quick response to signals from the brain.

Answers

Answer:
B

Explanation:
Thin membrane allow effective gaseous exchange to occur as the respiratory gasses can penetrate the membrane easily

What is a clue that the lac operon is on?

A.)Lactose is absent.

B.)The cell membrane changes.

C.)The repressor protein has fallen off the molecule.

D.)RNA polymerase is blocked by the repressor.

Answers

The correct answer is C.) The repressor protein has fallen off the molecule.

The lac operon is a regulatory system found in bacteria, which controls the expression of genes involved in lactose metabolism.

The lac operon consists of three main components: the structural genes (lacZ, lacY, and lacA), the promoter region, and the operator region. The regulation of the lac operon depends on the presence or absence of lactose and glucose.

In the absence of lactose, the lac repressor protein binds to the operator region of the operon, preventing the expression of the structural genes. This inhibits the production of enzymes involved in lactose metabolism.

However, when lactose is present in the environment, it acts as an inducer. Lactose is converted into allolactose, which binds to the repressor protein and causes it to undergo a conformational change. As a result, the repressor protein falls off the operator, allowing RNA polymerase to bind to the promoter region and transcribe the structural genes.

Therefore, the clue that the lac operon is on is the falling off of the repressor protein from the operator region, enabling gene expression and the production of enzymes required for lactose metabolism. Therefore, the correct answer is C.

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Is water and its properties important in homeostasis for humans ? explain.

Answers

Answer:

yes its important because...

Explanation:

Water is an essential feature of homeostasis in an organism. Water increases the volume of blood, which affects blood pressure and heart rate. Water dissolves gases and allow for efficient exchange and transport of oxygen and carbon dioxide. Water can be excreted, so it carries toxins that have been made water-soluble out of the body.

Explain how wetlands contribute to environmental health.

Answers

Wetlands play a vital role in the health of the environment. In addition to supporting a variety of organisms, they also reduce water erosion by trapping sediments. Wetlands help clean water by absorbing nutrients that are added to the water supply through agriculture and industry.

Answer: Wetlands play a vital role in the health of the environment. In addition to supporting a variety of organisms, they also reduce water erosion by trapping sediments. Wetlands help clean water by absorbing nutrients that are added to the water supply through agriculture and industry.

Ribulose biphosphate carboxylase is a chemical that aides in the process of photosynthesis. Ribulose biphosphate carboxylase breaks down carbon dioxide and bonds carbon atoms together to begin the formation of sugars. Without ribulose biphosphate carboxylase, photosynthetic organisms would not be able to produce as much food. Even though ribulose biphosphate carboxylase is involved in assisting chemical reactions, its chemical structure is unchanged.
Based on the information about the function of ribulose biphosphate carboxylase, what is the best classification of the chemical?
A.Carbohydrate B.Enzyme C.Lipid D.Nucleic Acid​

Answers

Answer:

The correct answer is - B. Enzyme.

Explanation:

RubisCo or ribulose bisphosphate carboxylase is an enzyme and most abundant protein on the earth that catalyzes the formation of the sugars from the CO2 by the photosynthesis process.

As it is given that in absence of the RuBisCo enzyme the cells plants or the photosynthetic organisms are not able to produce as much food as they produce in presence which means it has an essential role in increase the photosynthetic process to produce the food. By this, we can say it is an enzyme that helps in the process of photosynthesis.

Explain the role the ocean plays in the carbon cycle (think about the physical and biological processes that occur in the ocean)

Answers

Answer:

i need help with this exact question.

Explanation:

There are two types of cell reproduction

Answers

Answer:

the two types of cell reproduction are: Mitosis and Meiosis

Explanation:

Hope that helps!

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