in gel electrophoresis, a loading dye is usually added to the sample of dna to be loaded in the wells of the gel. why would this be important?

Answers

Answer 1

Answer:

Gel loading dye is typically made at 6X concentration (0.25% bromphenol blue, 0.25% xylene cyanol, 30% glycerol). Loading dye helps to track how far your DNA sample has traveled, and also allows the sample to sink into the gel. ... It is important to use the same running buffer as the one used to prepare the gel.


Related Questions

2. What is a gene? ________________________________________________________
3. What is the difference between gametes and body cells? ____________________________________________________________________________
4. Each person’s DNA is unique except for who?________________________________________________________________________
5. How does each gene code for a protein?__________________________________________________________________________________________________________________________________________________
6. Look at the picture of the nucleus, chromosome, and gene and infer what the relationship is of the three things? ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Answers

Answer:

2).a gene is a basic unit of heredity and a sequence of nucleotides in DNA that encodes the synthesis of a gene product, either RNA or protein

on what basis are populations assigned to the same biological species?

Answers

Answer: On What Basis Are Populations Assigned To The Same Biological Species? Answer A Very Similar Appearance Being Able To Interbreed And Produce Fertile Offspring Relating To The Environment In The Same Way Sharing A Common Ancestor Having 99% Of Their Genes In Common

Which does not contain cytoplasm? A. liver cell
B. palisade tissues C. red blood cell D. xylem vessel

Answers

Answer:

dfhzklgrwjhigwruighwreuygbwgerg

Explanation:

egg hjeghbhuwbguygb8qyugfbqey7g

Xylem because it is considered dead since it consists of dead narrow vessels and tracheids

when individuals are more successful at & reproducing than others?

Answers

Answer:

Natural selection, Evolutionary mechanism in which individuals that are better suited to their environment survive and reproduce most successfully.

interphase definition ?

Answers

Answer:

The resting phase between successive mitotic divisions of a cell, or between the first and second divisions of meiosis.

Answer:

the resting phase between successive mitotic divisions of a cell, or between the first and second divisions of meiosis.

The shape of the earth’s orbit is known as______ it can change from almost a perfect circle to
The shape of earth's orbit is known as
elliptical (egg like).
O obliquity
O precession
O orbit
O eccentricity

Answers

Answer:

A.

Explanation:

Because the other ones do not make sense.

Arolium uses in a cockroach.pleasehelpme.​

Answers

Answer:

just run away ........ ok save yourself

It is used in locomotion

The cockroaches possesses smooth flexible pads found at the distal end of the leg on tarsus. The fifth segment is the most flexible, found at the distal end and is made of a broadly triangular adhesive pad called as the arolium. The arolium is the adhesive organ of the cockroach foot engaged when the legs are pulling and used in scaling and adhering to the smooth surfaces. So, arolium helps in locomotion

So think of arolium as cockroach organ lube

The diagram below represents two cells viewed using the same magnification with the same microscope. One possible conclusion that can be drawn about the activity of these two cells is that...

Answers

Answer:

more active transport occurs in cell A then cell B

Explanation:

hope this helps ;)

The possible conclusion that can be made drawn about the activity of these two cells is that more active transport occurs in cell A than in cell B. The correct option is B.

What is transport?

Active transport is a type of transport that cells use to move molecules up and down cell membrane concentration gradients.

Moving actively is carried out using a variety of membrane proteins known as carrier proteins that are connected to an energy source, such as ATP, for example.

The energy needed to move molecules or ions up or down a concentration gradient is provided by the hydrolysis of ATP during this process, known as active transport. The other transport is passive transport, which is reverse.

Therefore, the correct option is B. more active transport occurs in cell A than in cell B.

To learn more about transport, refer to the link:

https://brainly.com/question/2375465

#SPJ2

Why do scientisits use light rays to represent light rays

Answers

Light rays allow us to draw clear diagrams showing the motion of light, including reflection (like bouncing off mirrors) and refraction (the bending of light when moving from one transparent material to another). This is called ray tracing. ... Trying to show interference and diffraction is a limitation of using rays.

PLEASE HELP ME IM TIMED!

Answers

Answer:

D

Explanation:

Actually fat is the best source of energy, since it is time consuming to break down a molecule of fat, carbohydrate is now used as an alternative to that since the molecules are broken down quicker.

A man is running on the straight road with the uniform velocity of 3 m/s.calculate the acceleration produced by him.​

Answers

[tex] \huge \bf༆ Answer ༄[/tex]

The acceleration produced by that boy is Zero .

_____________________________________

Want to know how ? here's the reason : -

As he is running with a constant velocity, so there's no change in velocity and Acceleration is defined as " Rate of change in Velocity " and since there's no change in velocity and direction (because he is moving in a straight road) hence Acceleration is 0.

[tex]\large꧁ \: \frak{Eternal \: Being } \: ꧂[/tex]

Imagine looking through a microscope at a squashed carrot root tip. The chromosomes of many of the cells are plainly visible. In some cells, replicated chromosomes are aligned along the center of the cell. These particular cells are in which stage of mitosis

Answers

I just got a call back and got the email and it is ok for the one I can get in it for today so I’m gonna be home bye mommy bye bye love bye mommy love bye bro bro bye bye love bye bro bye love you bye love mommy mommy bye bye love bye bye mommy love bye bye love mommy mommy bye bye love bye bye mommy love bye bye love mommy mommy bye bye love bye bye love mommy mommy bye bye bro bro bye bye bro I’m bored I just woke you bye bro bro bye bye bro love you mommy love mommy bye love you mommy bye bye bro bro bye bye mommy bye bye love mommy mommy bye bye bro bro bye bye love you ook I hope I got a ride bye mommy bye bye love mommy mommy bye bye bro bro bye bro I’m sorry bro I’m so sorry bro bro bye bye bro bro bye bye love bye bro bye love you bye love bye mommy mommy bye bye bro bro bye bye mommy mommy love you bro love love you bro I miss

Currents at the surface of the ocean, like those in El Niño are moved mainly by
O heat
O wind
O salinity
O the mixing of warm and cold water

Answers

Answer: winds

Explanation:

The second one B. wind

The diagram above shows the cell cycle. As you can see from the diagram, the cell spends most of the time in _______________________ during which it undergoes rapid growth and the DNA is replicated in order to prepare for Mitosis

Answers

Answer:

Interphase

Explanation:

Interphase is the phase in the cell cycle in which rapid growth occurs and DNA is replicated.

fundamental similarity due to descent from a common ancestor is called

Answers

Answer:

fundamental similarity due to descent from a common ancestor is called homology.

Fill out the data table below with the range of each factor that will produce the optimal number of oxygen bubbles.

Answers

Answer:

B

Explanation:

Spindle fibers are composed of which type of protein?

Answers

Answer:

Hey there. The answer should be Tubulin.

Explanation:

Spindle fibres are formed from microtubules which are made up of a protein called Tubulin.

The tilt or obliquity of the Earth causes ______
O seasons
O eccentricity
O global warming
O night and day

Answers

Answer:

seasons

Explanation:

some parts of the earth are closer to the sun tan other parts leading to different seasons

2. What is a gene? ________________________________________________________
3. What is the difference between gametes and body cells? ____________________________________________________________________________
4. Each person’s DNA is unique except for who?________________________________________________________________________
5. How does each gene code for a protein?__________________________________________________________________________________________________________________________________________________
6. Look at the picture of the nucleus, chromosome, and gene and infer what the relationship is of the three things? ____________________________________________________________________________________________________________________________________________________________________________________________________________________________________

Please answer all questions

Answers

Answer:

1. generation or birth or gender) is a basic unit of heredity and a sequence of nucleotides in DNA that encodes the synthesis of a gene product, either RNA or protein.

2: 1. Body cells make up the body of any multicellular organism. On the other hand, a gamete is a sex cell that can either be a sperm or egg cell.

2. Body cells undergo the process of mitosis while gametes undergo a process called meiosis.

3. A body cell contains a complete number of chromosomes and is called a diploid cell while a gamete contains only half the number of chromosomes of its parent cell, and it is called a haploid cell.

3. Identical twins

4. nucleotide triplets

5. A gene is a sequence of nucleotide bases that specifies certain products; RNA or proteins. It is the segment of DNA that holds the information to build and maintain an organism's cell and also pass genetic traits to offspring. Hence, it is the molecular unit of heredity in living organisms.

The DNA is a long double-helical structure that stores the genetic information in our cells. It consists of two long polynucleotide chains composed of the four nucleotides subunits (A, C, G, T).

In order for the long strand of DNA to fit into the cell's nucleus, it is coiled around certain proteins called HISTONES to form Chromatin, which undergoes further coiling and organization to to linear CHROMOSOMES.

The chromosomes are structured within the nucleus, that are made up of the cell's genetic material (DNA). In eukaryotic cells, the nucleus houses the cell's DNA and directs protein synthesis (expression of the information encoded by genes).

Explanation:

Name three components of the cell membrane and explain how each contributes the semipermeable nature of the membrane.

Answers

The principal components of the plasma membrane are lipids ( phospholipids and cholesterol), proteins, and carbohydrates. The plasma membrane protects intracellular components from the extracellular environment. The plasma membrane mediates cellular processes by regulating the materials that enter and exit the cell.

What are 2 ways PCR is used in everyday life?

Answers

Answer:

including genotyping, cloning, mutation detection, sequencing, microarrays, forensics, and paternity testing. I hope this helps! Explanation:

You do NOT have a pair of which of the following organs?

A. Lungs (plural)
B. Ureters (plural)
C. *Urinary bladder (singular and we have only one of these)
D. Kidneys (plural)

how did this question possibly violate any guidelines?
lets see if this question stays put this time.
Quiz let is better

Answers

Answer:

c. Urinary bladder is correct

Explanation:

Which would be least likely to be affected by a density-dependent limiting 4
factor?
O a small population of tigers scattered across a wide area
O a rapidly reproducing population of bacteria
a large, dense population of fish in a pond
a population of birds with a high immigration rate

Answers

Answer: a small population of tigers scattered across a wide area

Explanation:

Hope this helps

Answer:

The answer is a. a small population of tigers scattered across a wide area

materials are cycled out by the chloroplast and mitochondrion​

Answers

Carbon dioxide, Water, Sugar And Oxygen.

During exercise, the blood is travelling more quickly around the body. This carries more oxygen to the exercise muscles. Describe other changes in the athlete's body that enable more oxygen to be taken in at the lungs.

Answers

Answer:

your heart rate goes up explaining why the blood is traveling quicker. this causes you to breath faster meaning bring more oxygen to the blood in your lungs faster. your blood pressure also goes up from the increase in heart rate.

Answer:

the breathing rate and volume of each breath increases to bring more oxygen into the body

Explanation:

it removes the carbon dioxide produced. the heart rate increases, to supply the muscles with extra oxygen and remove the carbon dioxide produced.

which region of the brain contains gyri and sulci?

Answers

Answer:

The cerebral cortex, or the outer layer of the cerebrum

Explanation:

Which type of tooth is used to fragment foods into ever-smaller chunks by shearing, slicing, crushing, and griding

Answers

It’s the Premolars (bicuspids)

lon khoáng nào đóng vai trò đặc biệt quan trọng trong quá trình đông máu?

Answers

Answer:

1. Xét nghiệm yếu tố đông máu để làm gì?

Để đánh giá khả năng đông máu như thế nào và quá trình đông máu kéo dài trong thời gian bao lâu, các bác sĩ sẽ tiến hành xét nghiệm các yếu tố đông máu.

Quá trình đông máu sẽ bảo vệ cơ thể bạn khi bị chảy máu. Tuy nhiên, nếu một cục máu đông hình thành trong tĩnh mạch bình thường thì đây là điều hết sức nguy hiểm vì có thể khiến cho dòng máu bị chặn đến tim, não, phổi.

Khi thực hiện xét nghiệm các yếu tố đông máu, sẽ biết được nguy cơ bạn bị chảy máu quá nhiều khi bị thương hay không hoặc có nguy cơ bị đột quỵ không.

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2. Các yếu tố đông máu gồm những gì?

Fibrinogen

Fibrinogen là yếu tố ảnh hưởng lớn đến quá trình đông máu

Sau đây là các yếu tố ảnh hưởng đến quá trình đông máu:

I - Fibrinogen: Fibrinogen là huyết tương có trọng lượng phân tử là 340.000, hòa tan được. Yếu tố này có mặt trong huyết tương với nồng độ là từ 100-700mg/ 100mL. Đa số Fibrinogen được tạo ra ở gan, vì thế đối với những bệnh nhân bị bệnh gan thì lượng Fibrinogen giảm trong máu tuần hoàn, sự đông máu bị ngăn cản.

II- Prothrombin: Prothrombin là protein huyết tương có trọng lượng phân tử là 68.700, có mặt trong huyết tương với nồng độ là 15mg/100mL. Gan sản xuất Prothrombin liên tục, chính vì vậy nếu gan bị suy yếu, lượng prothrombin sẽ giảm, gây ức chế sự đông máu.

III- Thromboplastin mô: Yếu tố này tham gia vào cơ chế đông máu ngoại sinh, thay thế phospholipid tiểu cầu và các yếu tố huyết tương. Bên cạnh đó, thromboplastin còn có tác dụng chống nhiễm khuẩn.

IV- Ca++: Quá trình đông máu không thể không có mặt của loại ion này.

V- Proaccelerin: Khi có nhiều ion Ca++ thì yếu tố này mất hoạt tính. Khi không có proaccelerin, người ta điều chế huyết tương bằng cách để lâu huyết tương lấy từ máu chống đông với oxalat.

VII- Proconvertin: Trọng lượng phân tử của yếu tố này là 60.000. Hoạt tính của yếu tố này trong huyết tương sẽ bị giữ lại trên màng lọc amiang;

VIII- Antihemophilic A: Để tổng hợp yếu tố này, phụ thuộc vào rất nhiều gen trong các nhiễm sắc thể khác nhau. Thường thì antihemophilic được tổng hợp chủ yếu từ gan, lá lách và hệ thống võng nội mô. Khi thiếu ion Ca++ thì yếu tố này mất hoạt tính. Đây là yếu tố chống huyết hữu B;

IX- Antihemophilic B: Chống huyết hữu A.

X- Stuart: Stuart có trong huyết tương, ở dưới dạng không hoạt động. Trong quá trình đông máu nội sinh có sự tham gia của yếu tố này. Khi cho thromboplastin mô vào quá trình đông máu ngoại sinh, sẽ không còn yếu tố stuart.

XI- Plasma Thromboplastin Antecedent (PTA): Quá trình khởi phát đông máu nội sinh không thể thiếu yếu tố PTA.

XII- Hageman: Động lực để tạo thành một loạt phản ứng dẫn đến đông máu là sự tiếp xúc giữa yếu tố XII với mặt trong mạch máu tổn thương cùng sự có mặt của phospholipid tiểu cầu. Bên cạnh chức năng hoạt hóa hệ đông máu, Hageman còn hoạt hóa hệ đông máu, hệ bổ thể và hệ chống đông.

XIII - Fibrin Stabilizing Factor ( FSF): yếu tố này có hoạt tính bền vững trong huyết tương, ổn định fibrin.

Explanation:


I’ll give brain

Drag the tiles to the correct boxes to complete the pairs.


What is the function of each lymph organ?

The last drop-down box is spleen which you can’t see in the picture.

Answers

Answer: The primary lymphoid organs are the red bone marrow, in which blood and immune cells are produced, and the thymus, where T-lymphocytes mature. The lymph nodes and spleen are the major secondary lymphoid organs; they filter out pathogens and maintain the population of mature lymphocytes

Explanation: hope this helps bye :)

CAN SOMEONE PLEASESS HELP ME!! Thank uu!!!

Answers

The first one is D and the ones with lowering can help you with the second one either one should work
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