In the pulse-chase experiment to track the fate of proteins that are labeled during the pulse, the cells are washed and re-cultured (“chased”) by incubating them in radioactive amino acids. This is why the chasing step is necessary.
Pulse-chase experiments are used in labeled compounds to follow the regular dynamics of cellular pathways and processes. In a cell, molecules are continuously synthesized and degraded at different rates. Changes in these molecule localizations and their expression levels over time can be observed by first “pulsing” or simply say exposing cells to a labeled compound, then successively exposing the cells to the same compound, unlabeled, which is called “chasing”.
Compounds being studied are commonly labeled or tagged with fluorescent dyes or radioisotopes. Microscopic techniques are used to detect the turnover visually. There are several protein labeling techniques for pulse-chase which have certain application potentials. Reporter fluorescence fusion proteins are frequently used, but they are expected to disrupt or harm the complexing behavior of the native target protein, be toxic to the cells themselves
It may also affect target stability for pulse-chasee experiments. Fluorescence reporter fusion proteins also cannot be satisfied. For the multicolor pulse-chase experiments, SNAP-tag® and CLIP-tag™ are considered very useful. It is possible to do sequential labeling with two or more fluorophores.
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hii please help i will give brainliest to whoever is correct! :)
Answer:
Answer down below!
Explanation:
Answers ranging from top to bottom:
GlucoseCarbon DioxideWaterOxygenWho believed intelligence consisted of one general factor, called "g," underlying all mental abilities?
O Robert Sternberg
O L.L. Thurstone
O Charles Spearman
O Howard Gardner
The organelle is common to many types of cells and is the primary site for storage of materials such as water, proteins, lipids, and cellular waste. The size of the organelle can vary between plants and animals depending on its general function. True or false?.
Answer:
I believe its True
The organelle is common to many types of cells and is the primary site for storage of materials such as water, proteins, lipids, and cellular waste. The size of the organelle can vary, and it is a vacuole. This is the true explanation.
What are the different functions of the cell organelles?The cell organelles are different in different organisms, such as plants, animals, yeast, etc., because the cell organelles and their functions vary too. The plant cell has a large vacuole, which contains water, proteins, lipids, cellular waste, etc., it has mitochondria, a nucleus, etc., and the function of the vacuole is different from that of the animal cell vacuole. The vacuole of the plant cell is larger than that of the animal cell because the plant cell vacuole maintains the water pressure, but the animal cell does not do this.
Hence, the size of the organelle can vary between plants and animals depending on its general function, and it is a vacuole. This is the true explanation.
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More energy can be from fat molecule than from glucose molecule because the fat molecule contains more
Answer: Hope this helps.
Explanation: Triglycerides store about twice as much energy as carbohydrates. Triglycerides are made of glycerol and three fatty acids. Because there are so many carbons in three fatty acids, and each 2-carbon unit can enter the citric acid cycle, one fatty acid molecule can generate much more ATP than one molecule of glucose.
Answer:
because the fat molecule contains more kilocalories per gram
Article of plastic in the ocean.
Why or how does the article occur ?
Also provide a brief personal reflection on the article that you have given me
Earth's last sink for plastic waste, the oceans, receive the majority of it from the land. Major rivers operate as conveyor belts, gathering up debris as they flow downstream and carrying it to the ocean. Many pieces of plastic waste that are floating at sea stay in coastal waters.
Why is plastic in the ocean happening?Plastic garbage is transported through drains, streams, and rivers by wind and rain. Ocean access is via drains! Another significant factor is careless and incorrect garbage disposal; unlawful rubbish dumping significantly contributes to the plastic pollution of our oceans.Being such a persistent contaminant, plastic waste is particularly harmful. Decomposition of plastic products might take hundreds of years. Both people and animals are at risk from this garbage. In the debris, fish become entangled and hurt, and some animals mistakenly eat things like plastic bags thinking they're food.To learn more about : Plastic pollution
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Which soil (s) would have a deep O horizon (deciduous forest / desert / rainforest/ grassland)?
The temperate grassland soil is found to have a very deep O horizon.
How the grassland soil with deep O horizon.A layer of soil deep layer of O which enormous nutrients in large quantities are embedded in the temperate grassland.
On the contrast, the deciduous forest, rainforest and desert soil does not have high nutrients when compared to the ones in the grassland.
Soil refers to the topmost layer of the earth crust. There are different types of soil. These include the following:
Loamy soilSandy oilClay soil.So therefore, a dark top and deep layers of O horizon are found in the grassland.
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When neurons in your hypothalamus sense that blood pressure is low and that the body needs more water, it will send a signal to your pituitary gland, telling it to release anti- diuretic hormone. This hormone will instruct your kidneys to absorb less water/ leave more water in the bloodstream. All of the following systems are directly involved in this example of osmoregulation EXCEPT:
A.nervous
B. endocrine
C. urinary
D. circulatory
E. digestive
what number represents the subscriot 5h2
lysozyme, an enzyme found in human saliva, tears, and other secretions, catalyzes the hydrolysis of the beta-1,4-glycosidic linkages and peptidoglycan. predict the effect of this enzyme on bacteria and how it may be involved in human health.
lysozyme, an enzyme found in human saliva, tears, and other secretions, catalyzes the hydrolysis of the beta-1,4-glycosidic linkages and peptidoglycan. predict the effect of this enzyme on bacteria and how it may be involved in human health.
lysozyme, an enzyme found in human saliva, tears, and other secretions, catalyzes the hydrolysis of the beta-1,4-glycosidic linkages and peptidoglycan. This enzyme degrades the bacterial cell wall but it is suitable for human health by preventing bacterial infection.
What is hydrolysis?
The cleavage of biomolecules known as biological hydrolysis occurs when a water molecule is consumed in order to cause the division of a bigger molecule into its component parts. Saccharification is the process of hydrolyzing a carbohydrate into its individual sugar molecules, as occurs when sucrose is broken down into glucose and fructose.
The beta one for, say, acidic connections, an urgent analogy that will demolish the bacterial cell wall. By destroying their peptidoglycan, it breaks down the bacterial cell wall. In addition, since we lack peptidoglycan in our bodies, an enzyme that kills bacteria won't have the same impact on human cells. This is excellent for human health since it can help stop bacterial infections. Because it will presumably destroy microorganisms, we detect it in human secretions that come into contact with the environment, such as tears and saliva.
Hence . lysozyme degrades the bacterial cell wall but it is suitable for human health by preventing bacterial infection.
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why do cells need energy to divide?
s a researcher in a protein lab, you analyze two different proteins for their shape and the amino acids they contain. your results show that the two proteins have exactly the same numbers and types of amino acids, but they have different shapes.
According to the given statement Each protein has a unique linear sequence of amino acids.
What are amino acids ?Amino acids are the building blocks of proteins. Life's essential building blocks are proteins and amino acids. The breakdown or digestion of proteins produces amino acids as waste products. The human body uses amino acids to make proteins that help with food dissection.
Where can you find amino acids in the body?Three amino acids—valine, leucine, and isoleucine—known as branched-chain amino acids (BCAAs) have a branching chemical structure. A common component of muscle proteins, BCAAs promote the body's natural process of muscular growth and give us energy while we exercise.
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What is ‘making applesauce’? national geographic
The making of applesauce simply refers to the process of preparing a nutritious meal majorly with apples and water.
How is applesauce made?An applesauce is made by cooking some sort of apples such as McIntosh or even golden delicious and adding some spices to it. Some of the spices which is used in this process to make the sauce of apple include nutmeg, cinnamon and so on and so forth. It is a nutritious meal or juice which is highly rich in vitamins.
In addition to the above, the making of apple of applesauce could also be be done by peeling the apples, or it may be left unpeeled, some sweet ingredients can also be added to it.
Apples are fruits which belong to a class of food know as the vitamins or the body regulating and protective food. When fruits are consumed in the appropriate quantities, it helps prevent the body against diseases.
So therefore, the making of applesauce is done by the addition of peeled or unpeeled apple with water and other ingredients.
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Using food pyramids, explain why a vegetarian diet is more "ecologically responsible."
In comparison to a meat-based diet, a vegetarian diet uses less land and water and emits less greenhouse gases. As a result, it is frequently regarded as a greener choice.
What can you eat on vegetarian diet?Choose a range of healthful tree foods, such as whole vegetables and fruits legumes and nuts, and whole grains, to make the most of a vegetarian diet. Reduce consumption of unhealthier options including refined grains, fruit juices, and beverages with added sugar.
What is a true vegetarian diet?A vegetarian does not consume any meat at all, not even fish or fowl. The diet of a lacto-ovo vegetarian includes both dairy and eggs. A lacto vegetarian consumes dairy but avoids eggs. Ovo vegetarians consume eggs but not dairy.
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ATP to your cells is a
Answer:
Adenosine triphosphate (ATP) is the source of energy for use and storage at the cellular level
In what phase of meiosis are tetrads of homologous pairs lined up along the center of the cell and attached to the spindle fibers?
Anaphase I
Metaphase II
Metaphase I
Anaphase II
Answer:
Metaphase I
Explanation:
Hope this helps.
Industrial agriculture is most
common in
A. developed countries
B. Ethopia
C. South Africa
D. Nigeria
Answer:A
Explanation:because they are developed so they have the financing for it
what is the polypeptide component of lipoprotein called? what is the polypeptide component of lipoprotein called? prolipoprotein apolipoprotein prelipoprotein exolipoprotein
polypeptide component of lipoprotein is called Apolipoprotein
Apolipoprotein Apolipoprotein (Apo), a protein found in plasma lipoprotein, has the ability to bind and transport blood lipids to various human organs for utilisation and metabolism. The liver and small intestine both contribute to its primary site of synthesis. A collection of plasma proteins known as apolipoprotein is linked to a number of illnesses, including hyperlipidemia, atherosclerosis, Alzheimer's disease, and diabetes. Accurate identification and classification of apolipoproteins are important in order to study the function of apolipoproteins and create efficient targets for diseases associated with them. Biochemical investigations can accurately identify apolipoproteins, but they are expensive and time-consuming.To learn more about Apolipoprotein refer;
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True or false? data showing that the bacteria were the first lineage to diverge from the common ancestor of all living organisms suggest that the archaea and eukarya are more closely related to each other than they are to the bacteria.
True, the archaea and eukarya are more closely related to each other than they are to the bacteria
The archaea, eukarya and bacteria are described by the Woese's tree of life.
What is Woese's tree of life?
This is a unique research program developed by Carl Woese, it is based on rRNA, for discerning bacterial relationships and constructing a universal tree of life. Woese's interest in the evolution of the genetic code led to him to investigate the deep roots of evolution, develop the concept of the progenote, and conceive of the Archaea.
The tree indicates that the common ancestor for eukarya and archaea is more recent than the common ancestor for all three domains.
Eukarya are therefore more closely related to the archaea than is to the bacteria.
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PLEASE HELP!! WILL GIVE BRAINLIEST TO FIRST CORRECT ANSWER!
what molecule is the oxygen used to create inside the cell?
what is the name of this process ? What is the purpose of the molecule that is produced ?
Answer:
Cellular respiration can occur both aerobically (using oxygen), or anaerobically (without oxygen). During aerobic cellular respiration, glucose reacts with oxygen, forming ATP that can be used by the cell. The purpose of the ATP is to store energy for future reactions or be withdrawn to pay for reactions when energy is required by the cell.
Identify two important steroids in the human body and briefly explain theirfunctions
Answer:
Corticosteroids, including most synthetic steroid drugs, with natural product classes the glucocorticoids (which regulate many aspects of metabolism and immune function) and the mineralocorticoids (which help maintain blood volume and control renal excretion of electrolytes)
what impacts on the lion food web would occur if the prey hunted was no longer available
Answer:
If an organism is removed from the food chain, it spoils the flow of energy and nutrient in the ecosystem. It disrupts the balance of the food chain. As a result, the organisms which depend on others for food will die due to starvation.
Explanation: Hope this helps ya'..! :)
Using food pyramids, explain why a vegetarian diet is more "ecologically responsible."
The responsibility to preserve and protect the environment is known as ecological responsibility. The majority of the time, ecological responsibility is related to businesses making sure that their operations always abide by environmental laws and regulations.
Explain about vegetarian diet is more Ecologically responsible?People can drastically minimize their consumption of land, water, and oil resources as well as the amount of pollution they might otherwise generate by selecting a vegetarian diet rather than one high in animal products.But when the study's 153 vegans, vegetarians, and omnivores were all included in, it became clear that eating meat was, on average, worse for the environment. When we think about food crops, there are other broad issues to keep in mind that could have a greater negative influence on the ecosystem.According to a thorough peer assessment of studies on vegetarianism, a non-meat diet will probably result in a person emitting less carbon dioxide than they would with a meat-based diet—roughly 1,200 pounds less carbon dioxide. That translates to a mere 4.3% reduction in emissions for the average person living in industrialized nations.
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A young woman suffering from prosopagnosia, an inability to identify faces, a disorder of object recognition associated with damage to the _____________ lobe of the brain.
A young woman suffering from prosopagnosia, an inability to identify faces, and a neurological disorder of object recognition associated with damage to the occipital lobe of the brain.
Prosopagnosia is an inability to identify faces.
This neurological disorder is also known as facial agnosia or facial blindness where a person fails to spot facial expressions.
People suffering from prosopagnosia can not judge a person's age, expression, or gender and even their own face while standing in front of a mirror.
Prosopagnosia can also manifest in other ways as well such as difficulty while recognizing objects including cars, houses, and trees.
This disease is associated with the occipital lobe of the brain which is associated with memory or facial perception.
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Which answer best describes the data in Graph B and E?
Considering the given graphs of the interactions of the population of organisms:
The answer that best describes the data in Graph E is Population 1 was stable until Population 2 began to increase, causing Population; option BWhat might be the cause of the population data in Graph E is an introduced species caused the decline of the native species; option C.The answer that best describes the data in Graph B is: As Population 2 increases, Population 1 increases. Population 1 depends on Population 2; option C.The type of ecological relationship that Graph B likely represents is: Commensalism between two organisms: option D.What is a population of organisms?A population of organisms refers to all the organisms of a given species that are found living in a particular area.
The population of organisms living in a particular area interacts with one another as well as with other organisms and their environment.
The interactions found between populations of organisms include:
Competition - in competition, organisms compete with one another for food, water, water, space, and mates.Commensalism - in commensalism, one organism benefits from the other while the other organism is neither harmed nor benefitted.Predation - in this interaction, one organism kills the other for food.Mutualism - in this interaction, both organisms benefit.In conclusion, interactions exist between populations of organisms in a given area or habitat.
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Dietary supplements and decongestants containing ephedrine have recently become more strongly regulated in the united states. Ephedrine is an extract of a plant belonging to which phylum?.
Ephedrine is an extract of a plant belonging to the phylum Gnetophyta.
In the field of pharmacy, ephedrine can be described as a kind of agonist drug that has majorly been used for hypotension and also been used as a dietary supplement. Ephedrine has also been used to treat allergic reactions.
Ephedrine performs its effect on the body of an individual by acting as a stimulant for the central nervous system (CNS).
Ephedrine belongs to the phylum Gnetophyta which are characterized as small, vascular plants.
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“Why is there so much peat in the peat/perfrost system? Minimum of 5 sentences
PLEASE HELP
There is so much peat in the peat /perfrost system as a result of the low oxygen concentration in the environment which leads to incomplete decomposition occurring.
What is a Peat?This substance has a soft and dark brown property and is formed from partially decaying organic matter and dead substances present in a long period of time.This could take a certain period of years and are also rich in carbon content.
Perma frost is referred to an area which is located on land or under the ocean in areas where there is a low concentration of oxygen as result of certain factors and properties.
Since we are aware that peat is formed partially decaying organic matter under low oxygen concentration, then it is therefore the reason why there is so much peat in the peat/perfrost system and is therefore the correct choice.
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Part C
Why does the thermometer in the black paper show a greater increase in temperature than the others?
Answer:
Because a black surface takes in/absorbs more radiation from the sun!!
Explanation:
Hope this helps, have a great day :))
Scientists have classified how many different living things
Answer:
2 living things
Explanation:
According to Scientists, living things are classified into two; Plants and animals.
Hope it helps
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Why do scientists think gases such as CO2 trap heat in the atmosphere?
Greenhouse gases are more complex than other gas molecules in the atmosphere, with a structure that can trap heat. Greenhouse gas molecules in the atmosphere absorb light, preventing some of it from escaping the Earth.
Carbon dioxide is Earth's most important greenhouse gas that works for absorbing and radiating heat. Greenhouse gases absorb heat radiating from the Earth's surface and even circulate it in all directions including back toward Earth's surface.
Carbon dioxide in the atmosphere warms the planet, causing climate change. Human activities have raised the atmosphere's carbon dioxide content by 50% in less than 200 years.
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