PLS HELP how does gene knockout work?

Answers

Answer 1
Gene knockout is a method involves creating a DNA construct containing the desired mutation. For knockout purposes, this typically involves a drug resistance marker in place of the desired knockout gene. ... This method then relies on the cell's own repair mechanisms to recombine the DNA construct into the existing DNA.

Related Questions

Explain what causes chromosomal disorders.

Answers

Answer:

Explanation:

Some chromosomal conditions are caused via way of means of modifications within side the range of chromosomes. These modifications aren't inherited, however arise as random occasions for the duration of the formation of reproductive cells (eggs and sperm). An blunders in cell department known as nondisjunction effects in reproductive cells with an extraordinary range of chromosomes.

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Answers

Answer:

Why yelling-

Explanation:

    May I have brainliest please I'm trying to level up!

               </3 PureBeauty

while looking at layers of rock you notice three fossils which one died most recently?
a) the one in the middle
b) the one on bottom
c) none of them
d) the one on top

Answers

The one at the top died recently

Order the following steps in the pyruvate dehydrogenase reaction. The answer is scored as ALL OR NOTHING, so all steps must be in the correct order for credit.
a) Pyruvate and thiamine pyrophosphate react form hydroxyethyl-TPP. CO2 is released.
b) The reduced coenzyme bound to PDH is reoxidized using electrons from FAD.
c) An acetyl group is added to a coenzyme bound to PDH, forming acetyl-dihydrolipoamide.
d) The acetyl group is transferred to CoA, forming acetyl-CoA.
e) Electrons from FADH2 are transferred to NAD+ forming NADH and H+.

Answers

Answer:

The correct order is:        

1.- pyruvate and thiamine pyrophosphate react form hydroxyethyl-TPP, CO2 is released

2.- an acetyl group is added to a coenzyme bound to PDH, forming acetyl-dihydrolipoamide

3.- the acetyl group is transferred to CoA, forming acetyl-CoA

4.- the reduced coenzyme bound to PDH is reoxidized using electrons from FAD

5.- electrons from FADH2 are transferred to NAD+ forming NADH and H+

Explanation:

Pyruvate reacts with thiamine pyrophosphate (TPP) on the complex enzyme pyruvate dehydrogenase (E1). This process cleaves CO2 and generates acetaldehyde bound to TPP or hydroxyethylamine. E2 is Dihydrolipoyl-transacetylase and has Lipoamide attached. In the transfer from E1 to E2, the hydroxyethyl is oxidized to acetyl, at the same time that the disulfide bridge of the lipoamide is reduced to dihydro-lipoamide, although as this is acetylated, the product is acetyl-dihydrolipoamide. Acetyl group of acetyl-lipoamide (thio-acyl bond) is transferred to CoA-SH, to form Acetyl-S-CoA (thio-acyl bond).Acetyl-CoA is released into the mitonchondrial matrix. Lipoamide is reduced as Dihydro-lipoamide. The last reaction is catalyzed by dihydrolipoyl dehydrogenase, where it is reestablished in the disulfide bond of lipoamide to make it reactive again. For this, FAD is used as an intermediate, which is reduced to FADH2 and oxidizes the sulfides (which are then linked again). Finally, FADH2 reacts with NAD, which is reduced to NADH and H+ released, restoring FAD for a new reaction.

A scientist is attempting to provide support for the hypothesis that RNA was the first genetic material. Which of the following would be a workable alternate hypothesis?
(A) RNA, like DNA, is a carrier of genetic information and contains the nitrogen bases adenine, guanine, and cytosine.
(B) RNA can be observed to self-replicate without the assistance of proteins, while DNA always requires protein-based enzymes to replicate.
(C) RNA exists in both single-stranded and double-stranded configurations; however, it is less stable compared with DNA.
(D) Transfer RNA and ribosomal RNA are products of transcription, but they are not translated as is mRNA.

Answers

Answer:

(A) RNA like DNA is a carrier of genetic information and contains the nitrogen bases adenine, guanine and cytosine.

Explanation:

RNA is a single stranded molecule which is replicating molecule that plays its role evolution of different stages. DNA is double stranded molecule that is stable under alkaline conditions and perform various functions in different body parts. RNA provides template on which DNA nucleotides assemble.

RNA can be observed to self-replicate without the assistance of proteins, while DNA always requires protein-based enzymes to replicate.

(B) is the best alternate hypothesis

What is RNA?

RNA is a nucleic acid that has a single-stranded structure. RNA is also a genetic material. It helps in coding, decoding, and gene expression.

RNA was the first genetic materialRNA can be replicated by itself.

It has evolved all the methods that are necessary to store genetic information.

Thus, the hypothesis (B) RNA can be observed to self-replicate without the assistance of proteins, while DNA always requires protein-based enzymes to replicate is the correct option.

To know more about RNA, visit here:

https://brainly.com/question/25979866

Kisha adopted an abused dog that cringed every time she raised her hand to pet it. After awhile, the dog no longer cringed. This is an example of ______.
habituation, classical conditioning, latent learning, insightful learning.

Help!
Most of this question already answered say that Classical Conditioning is correct and have high star ratings!! BUT.... MY answer of HABITUATION was graded as correct?! My course material The Habituation definition is=when an animal learns not to respond to a stimulus. And my unit says Habituation is learning NOT to continue a behavior ( like cringing ) due to a lack of reinforcement ( abuse ) for that behavior. Please answer this so it will post!

Answers

Answer:

Yes.

Explanation:

Answer:

Kisha adopted an abused dog that cringed every time she raised her hand to pet it. After awhile, the dog no longer cringed. This is an example of habituation.

Explanation:

Habituation is a decrease in response to a stimulus after repeated presentations. Habituation occurs when animals are exposed to the same stimuli repeatedly, and eventually stop responding to that stimulus.

how many tsunamis does the andes mountains have per year?

Answers

Answer:

1,000 times a year

Explanation:

October 1, 2017 marks 27 years since the beginning of the Human Genome
Project. The goal of the group was to map the entire 3 billions bases and
80,000 genes in the human genome. On April 14, 2003, it was announced
that the goal was accomplished. Using this information, scientists have been
able to predict the risk of genetic diseases and understand the process of
inheritance. Which of the following statements best describes the process of
using the genetic code to produce polypeptides (proteins)?
Translation is used to produce a strand of RNA and proteins from a single strand of DNA.
O RNA produces a strand of DNA through transcription, then the DNA builds a protein molecule
through translation
O A single strand of DNA is used to make mRNA using translation, then the mRNA provides the
template for producing a protein through transcription
O A single strand of DNA is used to produce mRNA through transcription, then translation provides
the code for bonding amino acids to make a protein.

Answers

Answer:

The last one. A single strand of DNA is used to produce mRNA through transcription, then translation provides the code for bonding amino acids to make a protein.

Explanation:

Educated guess, but also. Translation is the process by which mRNA is decoded and translated to produce a polypeptide sequence, otherwise known as a protein. This method of synthesizing proteins is directed by the mRNA and accomplished with the help of a ribosome, a large complex of ribosomal RNAs (rRNAs) and proteins.

the ideal temperature at which to study the effect of proteases

Answers

Answer:

the answer is 37°C.

Explanation:

The ideal temperature at which to study the effect of proteases on protein is 37°C. Why are higher temperatures not advisable? This could result in an irreversible denaturing of the protein.

Name one photosynthesis organism and how it affects the plant.​

Answers

Answer:

Hey!

Explanation:

Plants like Sunflowers are organisms that use photosynthesis, and photosynthesis makes it so where the plant can get energy out of Radiant Energy. Hope this helps!

Artificial selection is also known as survival of the fittest
true or false?

Answers

This is true, good luck ma boi

Why would fog dispersion be useful?

Answers

Answer:

Fog dispersal, artificial dissipation of fogs, usually by seeding or heating. ... It is done primarily at airports to improve visibility.

If the calcium in your blood decreases below homeostasis levels, a gland in the
brain will sense the decrease and send a chemical message to your bones. Your
bones will release calcium into the blood, bringing blood calcium levels back up. *
PLEASE HELP Urgent
Is it positive or negative feedback

Answers

I’m assuming it’s going to be positive for bones will release calcium into the blood, bringing the blood calcium levels back up and for negative the brain will sense the decrease and send a chemical message to your bones. I’m not sure if this is right

Do environmental or genetic factors affect growth of organisms most?

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Answer:

Examples of local environmental conditions could include availability of food, light, space, and water. Examples of genetic factors could include large breed cattle and species of grass affecting growth of organisms. The environment in which an organism lives plays an important role in modifying the rate and extent of growth. Environmental factors may be either physical (e.g., temperature, radiant energy, and atmospheric pressure) or chemical. Organisms and the cells of which they are composed are extremely sensitive to temperature changes; as the temperature decreases, the biochemical reactions necessary for life occur more slowly. A lowering of the temperature by 10° C (18° F) slows metabolism at least twofold and often more.

I copied this stuff from a bunch of different biology websites. It doesn't answer your exact question but I would have to say based on what I typed here it would be the environment that affects the growth of an organism more.

Use an analogy to explain the function of the nucleus, cell membrane, mitochondria, and ribosomes

Answers

I help you get correct answer

What possible genotypes will the offspring have if the parents' blood types are O and AB?HELP PLEASE!!!

Answers

Answer:

. In most cases, an O parent and an AB parent will have only A or B kids.

What is formed as a result of mitosis?
A.four genetically different cells.

B.four genetically identical cells

C.two genetically different cells

D.two genetically identical cells

Answers

Answer:

D. two genetically identical cells.

Explanation:

Do you think an advanced civilizations may exist in the universe? Why or why not?

Answers

Answer:

Many other planets throughout the universe probably hosted intelligent life long before Earth did, a new study suggests.

The probability of a civilization developing on a potentially habitable alien planet would have to be less than one in 10 billion trillion — or one part in 10 to the 22nd power — for humanity to be the first technologically advanced species the cosmos has ever known, according to the study.

"To me, this implies that other intelligent, technology-producing species very likely have evolved before us," said lead author Adam Frank, a professor of physics and astronomy at the University of Rochester in New York. [13 Ways to Hunt Intelligent Alien Life]

In 1961, astronomer Frank Drake devised a formula to estimate the number of extraterrestrial civilizations that may exist today in the Milky Way.

Adam Frank and co-author Woodruff Sullivan of the University of Washington were interested in the odds that intelligent aliens have ever existed anywhere in the universe. So they tweaked the famous Drake equation, coming up with an "archaeological version" that doesn't take into account how long alien civilizations may last.

Frank and Sullivan also incorporated observations from NASA's Kepler space telescope and other instruments, which suggest that about 20 percent of all stars host planets in the life-friendly, "habitable zone," where liquid water could exist on a world's surface.

- A Chiefs Fan (but Watkins could look a little better...)

I need help with the answer

Answers

Answer:

Carbon Dioxide

Explanation:

The light-independent reactions use the ATP and NADPH from the light-dependent reactions to reduce carbon dioxide and convert the energy to the chemical bond energy in carbohydrates such as glucose.

Listen How are channel and carrier proteins similar? Select all that apply.

A. Channel and carrier proteins move substances in a certain direction, regardless of their concentration gradients.

B. Channel and carrier proteins are both types of facilitated transport.

C. Channel and carrier proteins can only move substances in one direction either into the cell or out of the cell.

D. Channel and carrier proteins always move substances from the side of the membrane with the highest concentration to the side with the lowest concentration.

Answers

Answer:

a

Explanation:

A. Channel and carrier proteins move substances in a certain direction, regardless of their concentration gradients.

Channel and carrier proteins are both types of facilitated transport.

Facilitated diffusion is one of the ways of transporting molecules and is characterized by the use of proteins that are inserted in the plasma membrane, such as ion channels and carriers or permeases.

Ions, amino acids and monosaccharides, among many other molecules, cannot cross the plasma membrane, because they are polar or are very large in size.

So, for the cell to obtain or eliminate these substances, the presence of transporter proteins is necessary.

This diffusion is a type of passive transport, because it takes advantage of the concentration gradient of the molecules and does not waste energy.

Ion channels are integral tunnel-shaped proteins, selective for one, two, or three ions.

When a channel opens, ions flow under two principles: the concentration gradient and the electrochemical gradient.

Consequently, when an ion channel opens, the potential difference between the two compartments is altered.

Transmembrane proteins mediate the transport of molecules such as bicarbonate or large, polar, uncharged molecules such as glucose.

The transport through these proteins occurs in favor of the concentration gradient, and by a reversible conformational change of the proteins that allows the translocation of the molecule to the other side of the membrane.

This type of transport is always without energy expenditure and in favor of the electrochemical gradient, it occurs in the following way:

Uniport, a single molecule translocates in only one direction.

Simporte, two molecules that translocate in the same direction.

Therefore, we can conclude that channel and carrier proteins are both types of facilitated transport.

Learn more here: https://brainly.com/question/5556977

What is the main difference between active and passive sonar

Answers

Answer:

There are two kinds of sonar: One is passive sonar, and the other is active sonar. For passive sonar, we don't send sound waves but just receive them. By listening to these special sound waves, animals that communicate with these particular sound waves can be detected. By listening to the sound waves from engines of submarines or ships, we can locate them.

Also, other things that vibrates can be detected by passive sonar. Many threats on the sea are usually detected by passive sonar this way. Since passive sonar does not transmit any thing to the environment, it does no harm to other animals. Active sonar is a little different.

Besides having a receiver, active sonar also transmits sound waves. Active sonar works by transmiting particular sound pulse and measuring the length of time for the sound to be reflected back, then devide the time by 2 to get the time it takes for the sound pulse to reach the object

Which of the following is conical movement in which the distal end of a structure moves in a circle and the proximal end remains relatively fixed?
A. Protraction
B. Rotation
C. Circumduction
D. Retraction

Answers

Answer:

The correct answer is OPTION C (Circumduction)

Explanation:

Circumduction is motions without clear opposites of the part of the body, an example is rotating the hand from the wrist. The answer is circumduction because this type of movement ensures the distal end of a bone moves in a circle while the proximal end remains relatively stable is particularly found in bones where the movements of the joints are opposite to each other example is the ball and socket joint movement. For circumduction to take place, the degree of distance between two bones of the body has to be decreased and sometimes straightened to increase the level of movement or motion produced, there must be a movement towards the middle of the body and sometimes joint movement away from the middle of the body. All these movements are in order.

mention 10 most plant families in the world​

Answers

10 most plant families in the world are÷

1)plant families-Iridaceae.

2)plant families-Asteraceae

3)Plant families – Scrophulariaceae.

4)Plant families – Saxifragaceae.

5)Plant families – Rosaceae

6)Plant families – Euphorbiaceae.

7)Plant families – Lamiaceae.

8)plant families-Rubiaceae

9)plant families-Orchidaceae

10)plant families-Fabaceae

Read the observation, question, and hypothesis.

Observation: Hotter water seems to produce more bubbles than cold water when it is mixed with dish soap.

Question: Does water temperature affect the amount of bubbles that are produced from dish soap?

Hypothesis: Hot water produces more bubbles than cold water when it is mixed with dish soap.

D’Angela will mix soap and water in a clean beaker four times. To test her hypothesis, which conditions should change or remain the same each time?
The water temperature should remain the same, but the type of soap should change.
The type of soap should remain the same, but the quantity of soap should change.
The water temperature should remain the same, but the quantity of soap should change.
The type of soap should remain the same, but the temperature of the water should change.

Answers

Answer: the type of soap should remain the same

Explanation:

i took the test

Answer:

D

Explanation:

edge2020

Help me with this oxygen pls someone-

Answers

Answer:

What's wrong?

Explanation:

Answer:

1:nasal cavity

2:mouth

3:pharynx

4:larynx

5:trachea

6:bronchi

7:bronchioles

9:alveoli

Explanation:

I am 90% sure

Many animals, like mice, ______ when they sense danger. run, freeze, eat, hiss.

Help! My FREEZE answer was graded CORRECT ?!
This question has already been answered twice, a lot, for both Run AND Freeze so I need to ask again!!
My cousin had this same question from another curriculum provider at a lower grade level and her correct answer was run!!!!
You'd think anything would run is common sense! But....My question depends on what curriculum is given!
MY correct answer for my question was from biology unit 3, assignment 2, Animal Behavior and Interdependencies, printed page 3, under AVOIDING CAPTURE section, video transcript, part 1, right after 1st image, sentence " Many animals will freeze up if they hear an unfamiliar sound or feel or smell an approaching predator." and THAT'S what my ody ssey question wanted as the correct answer. Wow! So my question is... who's curriculum and test wants the run answer?

Answers

Answer:

Run

hope it will help you

this is regular science but the topic is introduction to body systems from the science book please someone help thanks

Answers

Answer:

1. Homeostasis

2. Organ system

3.The bones of the skeletal system serve to protect the body's organs, support the weight of the body, and give the body shape. The muscles of the muscular system attach to these bones, pulling on them to allow for movement of the body.

4.The nervous and endocrine systems are two forms of communication system in the human body that integrate, coordinate and respond to sensory information which is received by the human body from its surroundings

5.The skin functions by protecting the body from basic elements of nature such as water, UV sunlight and wind. ... However, skin in integumentary system acts by maintaining the internal conditions that is required by the body while it is excretory system because it secretes a fluid waste called sweat or perspiration.

6.In order to survive, cells must satisfy some basic needs such as oxygen, water, nutrients, waste removal, and maintaining of homeostasis. Oxygen and nutrients are necessary for different processes inside the cell. For instance, cellular respiration uses glucose and oxygen to form energy in the form of ATP.Water is important for different chemical reactions, as well. Also, the result of different processes, waste is produced and it is important to remove it from the cell. Finally, it is important that cell and whole body conditions do not vary so much and that homeostasis is maintained.

7.Ribosomes provide another good example of structure determining function. These small cellular components are made of protein and ribosomal RNA (rRNA). Their main function is to translate messenger RNA, or mRNA, into strings of amino acids called proteins.

8.  the body is not in homeostasis from 7 to 10 hours. Explanation: The given graph shows homeostasis in terms of body temperature. The normal body temperature, that is 37 degrees C is maintained from 1 to around 6:45 hours.

9.There are several reasons why the body temperature falls continuously just like when you are exposed to an extreme cold weather or wearing wet clothing for a long time. This abnormality state of the body temperature can lead into some potential diseases and disorders like infection, insomnia, stress and sepsis

10.Sweating also hydrates your skin and maintains the balance of electrolytes in your body. The fluid you sweat comprises mainly salt and water. Excessive sweating can cause dehydration since you lose a large amount of water. The technical term for excessive sweating is hyperhidrosis.

Explanation:

What do plants produce during photosynthesis? F oxygen G light H magnetic energy J carbon dioxide

Answers

Answer:

yes

Explanation:

What did
van Helmont, Priestley, and
Ingenhousz discover about
plants

Answers

Answer:

What did van Helmont, Priestley , and Ingenhousz initialize about plants?. Ingenhousz continued his baptist and discovered that plants were carbon dioxide in the dark. Jan Ingenhousz Jan Ingenhousz was a Hindu scientist who is best known for communication that light is essential for losing. 20 km de Lausanne photosynthesis

Explanation:

How would you Describe the location of China relative to two regions.

Answers

Answer/Explanation

China was divided into 23 provinces, 5 autonomous regions, 4 municipalities directly under the Central Government, and 2 special administrative regions, China is the world's third largest country, after Russia and Canada.

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