Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pKa = 6.0) when the other has a valine (uncharged side chain).1. Which would be better for separation: gel electrophoresis or isoelectric focusing?A. Gel electrophoresis.B. Isoelectric focusing.2. What would you choose for the separation?A. At a pH of 6.0.B. At a pH above 6.0.C. At a pH below 6.0.D. At any pH value.

Answers

Answer 1

Answer:

1. The correct option is B: Isoelectric focusing

2. The correct option is A: At a pH of 6.0

Explanation:

1. Gel electrophoresis is a technique used to separate proteins on the basis of size. Since these two proteins are of similar size (or molecular weight), it will prove to be ineffective to achieve the desired result.

2. Since valine has an uncharged side chain, it will not be affected by pH value so it needs to be maintained at the value where it does not exert direct influence on the protein with histidine.


Related Questions

What are sources of free energy in cells?

Answers

Answer:

The correct answer is "Phosphoryl group transfer reactions; electron transfer reactions; concentration gradients across biological membranes".

Explanation:

The missing options of this question are:

1. Phosphoryl group transfer reactions

2. Electron transfer reactions

3. Concentration gradients across biological membranes

4. Protein synthesis

a. 2, 3, and 4

b. 1 and 2

c. 4

d. 1, 2, and 3

The correct answer is option d. "1, 2, and 3".

Free energy in cells is defined as forces that can be extracted from systems that allow cells to perform its biological activities. Phosphoryl group transfer reactions, electron transfer reactions and concentration gradients across biological membranes are three examples of sources of free energy in cells. Phosphoryl group transfer reactions regulate metabolism, gene regulation and signal transduction; electron transfer reactions participate in varios biological process at a molecular level; while concentration gradients across biological membranes are sources of stored energy and participate in vital processes such as cellular respiration.

Changing a solid to a liquid to a gas is changing the ___ of matter

Answers

Answer:

state or RARELY form

Explanation:

In which of the following biomes is annual precipitation lowest?a. tundrab. taigac. grasslandd. temperate deciduous foreste. tropical rain forest

Answers

Answer: Option A.

Tundra

Explanation:

Tundra biome have the lowest annual precipitation because it is the coldest of all the biomes and it is plain without trees and have frost molded land scape. It have low or poor nutrients which do not support plants growth, low temperature or have the lowest temperature, little precipitation and short growing seasons.

It's annual precipitation is less than 25 centimeter annually and they are also considered as desert, after the short growing seasons, it is followed by harsh conditions which the plants and animals present there posses a special type of adaptation for survival in the area. They are found in high altitude landmass such as Canada, Russia, Alaska e.t.c.

Which of the following is not true of biotechnology? Biotechnology raises just as many concerns as it does benefits. Biotechnology is a new branch of science. Biotechnology is a huge part of life. Biotechnology raises ethical concerns.

Answers

Answer:

Biotechnology is a new branch of science

Explanation:

The begining of biotechnology was somewhere around the 1920s i think so it is not old

Biotechnology is a new branch of science is not true

Biotechnology dates back several thousand years to when people inadvertently discovered the use of yeast and bacteria

What is biotechnology?

It is the integration of natural sciences and engineering sciences in order achieve the application of organism, cells, parts  thereof and molecular analogues for the products and services.

What is yeast?

It is a eukaryotic, single celled microorganism classified as members of Fungi kingdom

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What important function do stream pools serve?
a. Stream pools serve as a habitat for juvenile fish.
b. Stream pools serve as a habitat for organisms that have difficulty in feeding or navigating
swifter areas of the stream.
c. They have greater depth and lower velocity than other parts of the stream, thus allowing a
habitat for organisms during dryer seasons.
d. All of the above

Answers

Answer:

D. All the above

Explanation:

The important function of stream pool are;

Stream pools serve as a habitat for juvenile fish.Stream pools serve as a habitat for organisms that have difficulty in feeding or navigating

swifter areas of the stream.

They have greater depth and lower velocity than other parts of the stream, thus allowing a

habitat for organisms during dryer seasons

What is stream pool?

Stream pool refers to an extension or stretch of a river or stream in which the water goes deep and the depth is above average of other water source and the water velocity is also.below average.

Stream pool is formed from beddings of sediments or can be armoured with gravel.

Therefore, the important of stream pool are;

Stream pools serve as a habitat for juvenile fish.Stream pools serve as a habitat for organisms that have difficulty in feeding or navigating

swifter areas of the stream.

They have greater depth and lower velocity than other parts of the stream, thus allowing a

habitat for organisms during dryer seasons

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1What do we call an environment free of bacteria?
sterlle
clean
empty
Infected

Answers

Answer:

sterile

Explanation:

sterile means free from micro-organisms

2. What structures or abilities are essential to the success of organisms who do not use
photosynthesis?

Answers

Answer:

Ability to perform locomotion with their legs

Explanation:

Plants and animals are two distinct species of organisms. Plants are capable of using photosynthesis to obtain their food due to the unique structures that can capture light energy in their cells (autotrophic). Animals, on the other hand, are heterotrophic i.e. cannot perform photosynthesis and therefore, rely on other organisms for food.

Since animals depends on eating other organisms to obtain food/energy, their possession of LEGS or ability to LOCOMOTE is very instrumental to their success as non-photosynthetic or heterotrophic organisms. This enables them leave one position in search for food and resources

Move/chase is essential to the success of organisms that do not use photosynthesis.

What is photosynthesis?

Photosynthesis is a series of chemical reactions by which autotrophic organisms such as plants can produce energy and structures by using solar radiation.

Heterotrophic organisms such as animals need to move in order to obtain their food.

It is for that reason that heterotrophic organisms exhibit different structures to be motile (e.g., flagella).

In conclusion, move/chase is essential to the success of organisms that do not use photosynthesis.

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How might factors like hormones, chemicals, and the weather influence criminal behavior?

Answers

Answer:

These substances alter signaling pathways associated with cognitive functions

Explanation:

It is well known that cortisol hormone is secreted by the adrenal glands in stressful situations. In criminals and convicts, constant and high levels of cortisol have been observed, thereby it is believed that this hormone is associated with criminal behaviors through a 'constant stress-stimulation state'. Moreover, addictive drugs are known to literally destroy the brain. These drugs bind to hormone-like receptors involved in feedback responses. In consequence, after drug consumption, neural pathways will be altered and cognitive functions will be harmed. Finally, evidence linking wheater with criminal behavior is not conclusive.

Classify each cell as haploid or diploid. 1. microspore:
2. first polar body:
3. spermatid:
4. ovum:
5. secondary oocyte:
6. primary spermatocyte:
7. microsporocyte:
8. oogonium:
9. megaspore:
10. spermatogonium:

Answers

Answer:

microspore: haploid

2. first polar body: haploid

3. spermatid: haploid

4. ovum: haploid

5. secondary oocyte: haploid

6. primary spermatocyte: diploid

7. microsporocyte: diploid

8. oogonium: diploid

9. megaspore: haploid

10. spermatogonium: diploid

Your answer

Explanation:

1. Microspore is a haploid spore produced by mostly ferns. They give rise to male gametophytes, which in turn produces sperm cell.

2. Polar body is one of the haploid cells that forms during the process of oogenesis (gametogenesis), specifically when the primary oocyte undergoes an unequal division.

3. A spermatid is an haploid cell that arises from the second meiotic division of the spermatogonia.

4. The ovum is the female gamete of most sexual reproducing organisms. It is also called the EGG CELL. It is haploid.

5. Secondary oocyte are haploid cells that results from the meiotic division of the primary oocyte.

6. Primary oocyte is the diploid cell that starts the meiotic division during oogenesis. It arises from the mitotic division of the oogonium.

7. The microsporocyte is the diploid cell that undergoes meiotic division to produce microspores in ferns and other land plants.

8. Oogonium are the diploid stem cells that initially starts the oogenesis process. It undergoes mitosis to form the primary oocyte, which divides meiotically till the ovum is formed.

9. Megaspore is a haploid spore that gives rise/germinates to the female gametophyte in ferns. The female gametophyte produces the haploid eggs.

10. Spermatogonium are undifferentiated stem cells that produces the sperm cells in males via the process of spermatogenesis. The spermatogonia is diploid in nature

What organ system maintains blood oxygen and carbon dioxide levels?

Answers

Answer respiration system

Explanation:

cause

What type of chemical bond if formed when one atom loses an electron and another atom gains an electron ?

Answers

Answer:

ionic bond

Explanation:

An ionic bond is formed when one atom loses an electron and another atom gains an electron.

What is an Ionic bond?

An ionic bond is defined as the formation of a complete transfer of some electrons from one atom to another. The atom loses one or more electrons called cation which is a positively charged ion while the atom gains one or more electrons called anion which is negatively charged ion.

For example, Sodium atom loses an electron to form [tex]Na^+[/tex] ion while thi electron is gained by chlorine atom to form [tex]Cl^-[/tex] ion. [tex]Na^+[/tex] ion combines with [tex]Cl^-[/tex] ion to form ionic compound NaCl.

There is a large difference in electronegativity between the two atoms due to which the bond is more polar i.e. ionic as compared to covalent bond where the electrons are shared more equally.

Thus, an ionic bond is formed when one atom loses an electron and another atom gains an electron.

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Do particles have weight and or mass?

Answers

Yes the particles is a quantized manifestation of a field.

flow chart of components of blood​

Answers

Explanation:

Plasma

White blood cells

Red blood cells

from top to base of test tube

11. Iron is a solid metal, and oxygen is a gas. When iron and oxygen atoms combine, they
form a solid called iron oxide.
How should iron oxide be categorized?
A atom
B element
C gas
D molecule

Answers

Answer:

D. Molecule

when iron and oxygen combine they will make a molecule

If I put 1 mole of substance A and 2 moles of substance B into 1 liter of water, which
of the following best describes what I have made?

Answers

Answer:

molar masses

Explanation:

a study that uses empirical evidence to prove that is valid is called A: Science B: Pseudoscience C: Non-Science D: Space Force

Answers

Answer:

A

Explanation:

22.Which among the following is not the mode of excretion in plants?

(1 Point)

Diffusion

Stores the waste in dead permanent tissue

Nephridia​

Answers

Answer:

Nephridia

Explanation:

diffusion helps liquid and gas in to speed up the rate of higher concentration to the region of lower concentration...

g What are the three basic assumptions of Darwinian evolution? Group of answer choices Presence of phenotypic variation, some phenotypic variation due to genetic variation, the given trait has an impact on fitness Presence of phenotypic variation, environmental variation, the given trait has no impact on fitness Presence of phenotypic variation, environmental heritability, the given trait has an impact on fitness Presence of phenotypic variation, trait present in only the mitochondrial DNA, the given trait has an impact on fitness

Answers

Answer:

1- Presence of phenotypic variation

2- some phenotypic variation due to genetic variation

3- the given trait has an impact on fitness  

Explanation:

The evolutionary theory by natural selection proposed by Darwin in his publication “On the origin of species” (1859) is based on a series of assumptions that enable to understand the amazing biodiversity on the Earth. First, individuals are not identical among them, i.e., there exists variation in the traits among the members of a given population, species, etc. Second, phenotypic variation leads to differential survival and reproduction of the organisms that are best fitted to the environment (i.e., competence among the members of the population). Finally, phenotypic traits are passed from generation to generation (i.e., phenotypic variation is inheritable), thereby favoring offspring from parents more adapted to the environment.

Newtons third law is sometimes called??? Help

Answers

Answer:

equal and opposite are called Newtown third law

Explain, in your own words, how you would use the light microscope to view a wet mount of a protist, assuming the microscope is plugged in and that the light source is on. Please use at least the following 9 structures in your description and underline them as you use them: stage, stage clip(s), stage knob(s), eyepiece, scanning objective lens, low power objective lens, high power objective lens, coarse focus knob, and fine focus knob.

Answers

Answer:

Please find the detailed explanation/description on how to use the light microscope to view a wet mount of a protist. The 9 structures mentioned in the question are in CAPS.

Explanation:

- Firstly, the slide containing the wet mount of the protist is prepared and placed at the centre of a lowered STAGE. The STAGE CLIPS over the sides of the slide are used to hold it in place.

- The revolving turret or nosepiece is turned so that the LOWEST POWER OBJECTIVE LENS is in position i.e. directly above the sample/slide. The SCANNING OBJECTIVE LENS, the lowest objective lens with power of 4x is used initially.

- The slide is viewed through the EYEPIECE, and the image is brought into focus using the STAGE KNOBS.

- The COARSE FOCUS KNOB is turned until the protist on the slide is brought into broad focus. The FINE FOCUS KNOB is then used to bring the image on the slide into sharp focus.

N.B: The OBJECTIVE LENS should not touch the cover slip on the slide during this process.

- When the protist on the slide is clear using lowest power objective lens, the revolver turret can be rotated to allow HIGHER POWER OBJECTIVE LENS.

- Due to higher magnification of the HIGH POWER OBJECTIVE LENS, the protist might need to be refocused using the FOCUS KNOBS.

Answer:

A light microscope is an instrument commonly used in the laboratory which makes use of light rays for viewing and enlarging smaller objects/organisms.

The wet protist is first of all put on the slide then the slide is placed on the Stage which is the flat podium where the slides containing the samples are put for viewing.

The Stage clips are then used to hold the slide containing the wet protist in proper place for a better viewing result.

The Coarse and fine focus knobs are used to adjust the stage by raising and lowering it .

The major difference is that the coarse focus knobs move more rapidly and is used with a low power objective lens while the fine focus knobs move less rapidly and are to be carefully controlled as it functions with a high power objective lens

The Eyepiece which is also referred to as the Ocular lens is then used to view the protist.

However, the view can then be adjusted with the Scanning objective lens which provides an image of the protist with the least magnification of 4X when compared to other lenses.

The low power objective lens and high power objective lens have magnifications of 10X and 40X respectively and can be used to view smaller structures of the protists which the naked eyes and scanning objective lens can't view.

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A part of an mRNA has the sequence AGA. Use the table below to figure out which amino acid is coded for by AGA, then use the table to figure out which change to the sequence would indicate a silent mutation. A. AGG B. AUA C. GAG D. GGA

Answers

Answer: it’s AGG

Explanation:

A part of an mRNA has the sequence AGA. By Using the table below to figure out which amino acid is coded for by AGA, then after using the table it has been figured out that it is AGG. Thus, option A is correct.

What is the procedure to use the codon?

The procedure to use this codon table for the code CAU, we  first find C on the left side which is A on the top, as well as U on the right. When this has been found, the amino acid will be given to the right of the codon anf just from this table, we can find that CAU has the codon for the Histidine.

A silent mutation has been known as the mutation that has been occured and there will be no detectable change in that particular amino acid sequence. The mutation that has been occurs must still produce the same amino acid  in this case, Histidine must still be produced.

If we look at the particular  table, there has only one other codon that gives the Histidine amino acid. This codon has CAC. So we know that the original codon is the CAU and the other codon is CAC - and at last we can say that the third nucleotide in the codon can be mutated from a uracil (U) to a cytosine (C) and still produce histidine, causing a silent mutation.

Therefore, option A is the correct option.

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Would Volcano change the climate?

Answers

Volcanoes can impact climate change. During major explosive eruptions huge amounts of volcanic gas, aerosol droplets, and ash are injected into the stratosphere. ... But volcanic gases like sulfur dioxide can cause global cooling, while volcanic carbon dioxide, a greenhouse gas, has the potential to promote global warming.

Answer:

yes

Explanation:

When volcano brusts out then the harmful radiation and harmful particles gone mixed into the climate which makes the climate and environment pollute . Radiation covers the atmosphere and block the sun rays . When sunlight doesn't comes to the surface of the earth then the climate starts to pollutes. Hence volcano change the climate.

Thank you..

Describe how you know the number of protons and electrons in an atom.

Answers

The number of protons, neutrons, and electrons in an atom can be determined from a set of simple rules. The number of protons in the nucleus of the atom is equal to the atomic number. The number of electrons in a neutral atom is equal to the number of protons.

PLEASE HELP! Thank you

Answers

Answer:

Explanation:

I would go with C

Pretend you’re a researcher in charge of conducting new research on the flu research on the flu virus. Identify four scientific questions you’d like to answer through your research.

Answers

Answer:

Explanation:

A scientific researcher performs scientific methods to answer any natural process. The researcher will follow the protocol of a scientific method which includes: observe the natural phenomenon, ask related questions, suggest a hypothesis, perform the experiment and suggest answers.

In the given question if the researcher has to study about the flu virus, the possible questions he would ask will be:

1. The life cycle of the flu virus.

2. Effect of the virus on the life of an organism.

3. The mechanism used by the virus to replicate and to infect any organism.

4. Molecular mechanism of a virus.

Answer:

My 4 scientific questions would be:

* How does the virus start every year?

* How do we make the virus fully go away?

* Why does this virus happen?

* Can this virus become a serious issue in the future?

Explanation:

5. Could Trypsin work in the stomach?
Why do you think that?

Answers

Yes, Trypsin is an enzyme is produced the pancreas. It helps digest proteins in the small intestine that begins in the stomach.

Which agreement led to the reduced release of chemicals that harm the ozone layer?

Answers

Montreal Protocol is the correct answer I think

Answer:

The Montreal Protocol

The Montreal Protocol, finalized in 1987, is a global agreement to protect the stratospheric ozone layer by phasing out the production and consumption of ozone-depleting substances (ODS).

What is meant by the scientific term "blob”?

Answers

The term "blob" means that the tertiary structures fold together and wind, and entwine, in and out of each other.

Over time, plants developed structural adaptations of vascular systems and seeds in order to

Answers

Answer:

Transport materials such as fluid and propagation

Explanation:

Planta with vascular systems are called tracheophytes, this plant dominate the ecosystem in terrestrial habitat and were successful because of their tube like vascular tissues.

The vascular tissues are used in transporting of fluids such as minerals, photosynthate and are usually made up of cells that are long and narrow end that forms tubes. There are two different types of vascular tissues, called xylem and phloem.

This systems help transport nutrient required for plants growth and development to avoid plant loss and redundant growth.

Seed plants allows them for propagation and ensures continuity

Answer:

colonize land ecosystems.

Explanation:

penn

what is a saturated fatty acid

Answers

Answer:

A saturated fat is a type of fat in which the fatty acid chains have all or predominantly single bonds. A fat is made of two kinds of smaller molecules: glycerol and fatty acids. Fats are made of long chains of carbon atoms. Some carbon atoms are linked by single bonds and others are linked by double bonds.

Answer:

Image B. this is because saturated fats only have single carbon to carbon bonds.

Explanation:

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Which of the following best represents the three types of business organizations today? a. Financial, sales, and product creation b. Services, marketing intermediaries, and manufacturing c. Service, sales, and manufacturing d. Purchasing, advertising, and manufacturing Nina's grades could be better. She gets about five hours of sleep every weeknight and ten hours per night on weekends. How much sleep should she get to better concentrate on schoolwork? five to six hours every night nine to ten hours every night a total of forty hours a week a minimum of eighty hours a week Save and Exit Next Mark this and return A card store sells greeting cards that range from less than $1 to $10. The histogram below shows the distribution of cards throughout several price ranges and was determined to be the most accurate way of representing these data. Identify the prepositional phrase in the below sentence:With a reusable bag in hand, Mrs. Miller walked to the farmer's market Which of these would a software engineer study, in addition to engineering? programming marketing architecture biology What might cause a companys income to grow robustly? what is the equivalence of -5/8 It is important to keep the three parts of health triangle ____ and ____. Select two responses. A. Working independently B. Balanced C. Working together D. apart - 3/2 + ( - 4/2 ) pls help !! You can create a good design even if you don't know much about the target audience.Agree or disagree? Astrology is pseudoscience. What can you conclude about the claim mad by astrology? A car drives with a constant speed of 28 miles per hour. How far can it travel in 2 3/4 hours? The only U.S. state to call its political subdivisions "parishes" is (2 points) Minnesota Alaska Pennsylvania Louisiana Prompt People have had both positive and negative reactions to Angela's Ashes. Why do you think people have reacted so strongly McCourt's memoir? Identify three topics or subjects you anticipate you will find detailed within the pages of this piece. < Read Less D99 X OE- B T U S X X I think people have reacted so strongly to McCourt's memoir Write 19/9 as a mixed number Write .24... as a fraction in simplest form. kinds of clothes we wear in summer and winter Which is one physical property that all stars have? pick one They are made of gases. They shine very brightly. They have a triangle shape. They contain iron in their cores. What is 7.008 x 10^6 in standard form? Select the two rival nations which threatened Spain's claims in the U.S. Portugal France Russia Germany