the bradford assay only works at low protein concentrations

Answers

Answer 1

Answer:

Dilute the sample

Explanation:

Answer 2

The statement is not entirely accurate. The Bradford assay is commonly used to determine protein concentrations in a sample.

However, it is true that the Bradford assay is more accurate and reliable for measuring protein concentrations at low to moderate levels rather than at very high concentrations.

The Bradford assay is based on the binding of Coomassie Brilliant Blue dye to proteins. The dye undergoes a color change from brown to blue when bound to proteins, allowing for the measurement of protein concentrations. At low protein concentrations, the binding of the dye to proteins is well-calibrated and results in a linear relationship between protein concentration and color intensity, making it suitable for accurate quantification.

At high protein concentrations, the binding capacity of the dye may become saturated, leading to non-linear and less accurate measurements. The excess protein can also cause interference and affect color development. In such cases, dilution or alternative protein quantification methods may be more suitable, such as the BCA assay or the Lowry assay.

Therefore, while the Bradford assay can be used to measure protein concentrations across a range of concentrations, it is generally more reliable for low to moderate protein concentrations.

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Related Questions

`Carbon Dioxide and Water are the _______ in a chemical reaction. 2. Reactants are also called the starting _______ in a chemical reaction.: Reactants are also called the starting _______ in a chemical reaction. 3. This type of energy comes from the sun: a This type of energy comes from the sun 4. You get this type of energy from food: b You get this type of energy from food 5. The process by which plants use the sun to produce glucose and oxygen.: The process by which plants use the sun to produce glucose and oxygen. 6. The products of photosynthesis: The products of photosynthesis 7. The location of photosynthesis in plant cells: The location of photosynthesis in plant cells 8. Another name for glucose is: Another name for glucose is 9. Energy is provided by this cell organelle during cellular respiration: Energy is provided by this cell organelle during cellular respiration 10. The largest source of energy on earth comes from the: d The largest source of energy on earth comes from the Column B a.Chloroplast b.Ingredients c.Reactants d.Sun e.Radiant energy f.Sugar g.Chemical energy h.Glucose and Oxygen i.Mitochondria j.Photosynthesis

Answers

the answer would be elector literally

in which time period did the first animals that could live both in water and on land appear

Answers

510 million years ago.

the idea of arranging the elements in the periodic table

Answers

Elements are arranged from left to right and top to bottom in order of increasing atomic number. Order generally coincides with increasing atomic mass. The rows are called periods.

When a hurricane approaches land, people living directly on the
coast should always evacuate when told to do so by authorities.

Answers

Answer:

True

Explanation:

When a hurricane is dangerous enough that authorities say to evacuate, you should for your own safety.

 Write the chemical compounds and names for the following ionic compounds. Magnesium and phosphorus 

Answers

Answer:

Magnesium is Mg.

Phosphorus is P.

A Herbig-Haro (HH) object is:_:_________.
a. a small galaxy just outside the outer edge of the Milky Way
b. where a jet from a star in the process of being born collides with (and lights up) a nearby cloud of interstellar matter
c. a part of a giant molecular cloud where molecules containing two hydrogen atoms form
d. a star that has too little mass to start fusion in its core
e. cluster of very low-mass stars, observable only with infrared radiation

Answers

Given what we know about Herbig-Haro (HH) objects, we can confirm that they are born from the collision between a jet from a star and clouds of interstellar matter.

What are Herbig-Haro (HH) objects?To put it simply, a Herbig-Haro (HH) object is what we observe as a bright spot in distant space. Upon closer observation we can observe that these giant bright patches are not lone objects, but in fact immense groups of interstellar matter.

How Herbig-Haro (HH) objects are formed.The formation of one of these interstellar groups requires an immense amount of energy. This energy come from the speed at which the materials that form these objects collide.As stated in the question, the jet released from a star in the process of being born smashes into a giant cloud of dust and interstellar material to form the Herbig-Haro (HH) objects. This collision happens at hundreds of km/s. To put it into perspective, it would be roughly 500,000 miles per hour.

Therefore, we can confirm that option b, which states, "where a jet from a star in the process of being born collides with (and lights up) a nearby cloud of interstellar matter", is the correct choice for the question pertaining to Herbig-Haro (HH) objects.

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Based on the graph, what is the approximate half-life of the reaction shown below

Answers

Answer: 2.1 minutes

Explanation: Based on the graph, what is the approximate half-life of the reaction shown below

molecules diffusing with the concentration gradient through a protein channel is

Answers

Answer:

Molecules move up the concentration gradient in facilitated diffusion which requires ATP to be used as molecules naturally move from high to low concentration

Explanation:

what is the chemical formula of tetraphosphorus heptasulfide?

Answers

Answer: P4S7

Explanation:

What is the molarity of a 75.0 mL solution that contains 0.225 g of
potassium nitrate?

Answers

Answer:

M=0.0297M

Explanation:

we know that molarity equals number of mole of solute over the volume of solution.

M=n/V but n=m/M

n=0.225g÷101g/mol =0.00223mol

M=0.00223mol/0.075l

M=0.0297M

Answer:

0.0297M

Explanation:

We will use the formula M= moles of solute/lites of solution.

First we need to figure out the Moles of the solute, to get this we first need the molar mass of KNO3 (potassimum nitrate).

The Molar Mass of potassimum nitrate is 101.103 g/mol
K - 1 x 39.09
N - 1 x 14.006
O - 3 x 15.999
KNO3 - 101.103 g/mol

Now we get the moles of the solute by doing.
0225g/ 101.103g/mol = 0.00223 mol

Put the answer into the orginal equation to get 0.00223mol / 0.045 L
Which equals to 0.0297M. This is also the correct amount of significant figures.

A student makes an aqueous solution of sodium hydroxide. Which statement correctly identifies the two ions present in the solute of this mixture?

The sodium ion is negative, and the hydroxide ion is positive.

The sodium ion is positive, and the hydroxide ion is positive.

The sodium ion is positive, and the hydroxide ion is negative.

The sodium ion is neutral, and the hydroxide ion is neutral

Answers

The two ions present in the solution are:

1. Sodium ion, Na⁺

2. Hydroxide ion, OH¯

The correct answer to the question is the 3rd option. The sodium ion is positive, and the hydroxide ion is negative.

Ions are atoms or group of atoms which possess an electric charge.

Aqueous Sodium hydroxide, NaOH contains sodium ion and hydroxide ion.

The sodium ion is positive while the hydroxide ion is negative as illustrated below:

NaOH(aq) —> Na⁺(aq) + OH¯(aq)

From the illustration above, we can conclude that the correct answer to the question is the 3rd option. The sodium ion is positive, and the hydroxide ion is negative.

Please see attached photo for further details

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How do you prepare 500ml of 1.77M H2SO4 solution from an 18.0M H2SO4 Stock solution

Answers

Answer:

Take 49.2-ml of 18.0 M H2SO4 stock solution and pour it into a 500-ml volumetric flask. Fill to the 500-ml line with distilled water to make 1.77M H2SO4 solution.

Explanation:

use the formula M1V1=M2V2

volume must be in liters(L)

[tex](1.77)(500 \times {10}^{ - 3} ) = (18)v2 \\ 0.885 = 18v2 \\ v2 = \frac{0.885}{18} \\ v2 = 0.04916[/tex]

0.04916L × 1000 = 49.16 ml = 49.2 ml

(BRAINLIEST AND 50 POINTS) no links
Name one organ system that isn't the Nervous system, Respiratory system, Skeletal system, and Circulatory system, and explain how can that system help keep you safe during a forest fire.

Answers

Answer:

Endocrine system

Explanation:

Endocrine system influences the function of the body's hormones via internal glands that are secreted into the circulatory system.

in a forest fire, your endocrine system would trigger the release of  adrenaline which is the fight or flight hormone. it would affect your heart rate, metabolism, oxygen intake, and blood flow allowing you to move quicker and escape the fire

the ____ changes when electrons are gained or lost.

Answers

Answer: Matter changes when electrons are gained or lost.

Explanation:

The charge on an atom  changes when electrons are gained or lost during chemical changes.

What is an atom?

An atom is defined as the smallest unit of matter which forms an element. Every form of matter whether it is  solid,liquid , gas consists of atoms . Each atom has a nucleus which is composed of protons and neutrons and shells in which the electrons revolve.

The protons are positively charged  particles and neutrons are neutral and hence the nucleus is positively charged. The electrons which revolve around the nucleus are negatively charged and hence the atom as a whole is neutral  species and stable due to presence of oppositely charged particles.

Atoms of the same element are similar as they have number of sub- atomic particles which on combination do not alter the chemical properties of the substances.

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what describes the pool of electrons in metallic bonding?

Answers

In metallic bonding, the electrons are “surrendered” to a common pool and become shared by all the atoms in the solid metal. Metallic bonding is found in metals and their alloys. When the atoms give up their valence electrons, they form ions. These ions are held together by the electron cloud surrounding them.

What is gadolinium

And what would be a superpower that relates to it

Answers

Answer:

Chemical with symbol Gd on periodic table of elements. It reacts with oxygen.

Fire superpower i guess

Explanation:

The characteristics “fingerprints” of light produced when a substance is given energy are called…
A. Groundlings
B. Quantums
C. Spectrums
D. Amplitudes

Answers

A spectrum is a  pattern is unique to a substance and can be used identify it.

According to Max Plank, energy occurs in packets called quanta. Hence, energy is not continuous but takes on discrete values which are integer multiples of a fundamental amount.

Again, when a substance is given light energy, a characteristic fingerprint is obtained. This pattern is unique to the substance that has it. These characteristics “fingerprints” are called spectrums.

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A calcium atom has 20 protons, 20 electrons, and 20 neutrons. When calcium forms a positive two ion it's new composition is

Answers

Answer:

40

Explanation:

I'm not sure

I need help, plz help me with this problem

Answers

Answer:

It's b

Explanation:

I had the same exact question

Question 5 (Fill-In-The-Blank Worth 2 points)
(03.05 LC)

The number that represents a neutral pH is _______.

Numerical Answers Expected!

Answer for Blank 1:

Question 6(Multiple Choice Worth 2 points)
(03.05 MC)

Which item is made from an acidic ingredient?

Detergent
Soap
Ammonia
Vinegar
Question 7(Multiple Choice Worth 2 points)
(03.05 MC)

Which substances will make a salt when combined?

Vinegar and tea
Soda and wine
Soda and ammonia
Fertilizer and detergent
Question 8(Multiple Choice Worth 2 points)
(03.05 MC)

Which description is not a property of an acid?

pH lower than 7
Turns litmus paper blue
Sour taste
Corrosive
Question 9(Multiple Choice Worth 2 points)
(03.05 MC)

Complete the sentence. When an acid is added to water, hydrogen ions __________.

increase
decrease
stay the same
disappear

Answers

5. neutral pH is 7
6. vinegar
7. soda and ammonia
8. turns litmus paper blue
9. stay the same

3 examples of how you think cells work in living things.

Answers

Answer:

Cells help the human body in many ways, Red or white blood cells are very important and are commonly spoken when bringing up cells. Plants have cells as well plant cells have special organelles called chloroplasts, which create sugars also known as photosynthesis.

Explanation:

idrk if that helped much-

Write the chemical formula for the following ionic compound nickel (III) sulfate 

Answers

Answer:

Nickel(III) Sulfate Ni2(SO4)3 Molecular Weight -- EndMemo.

Explanation:

this will explain everything

help me please i dont understand

Answers

Answer:

1. Dissolving powder in milk - Chemical

  - It is chemical because the milk has changed on a molecular level

2. Toasting bread - Chemical

   - It is chemical because adding heat to the bread cooks it, therefore

     changing it on a molecular level

3. Melting cheese - Physical

   - It is physical because the physical appearance was the only change

4. Slicing apples or bannanas - Physical

   - It is physical because the physical appearance was the only change

5. Frying an egg - Chemical

   - It is chemical because new particles were formed

6. Milk souring - Chemical

   -  It is chemical because it is forming a new product (lactic acid)

Explanation:

Physical Change occurs when the particles of a substance become rearranged, but do not change into different particles.  

Chemical change occurs when a new substance is formed. This process is irreversable.

Answer:

give the preson above brainly bc there right

Explanation:

Which best describes an element?
a pure substance
a type of a mixture
a pure compound
an impure substance

Answers

An element is a pure substance

Answer:

An impure substance

Explanation:

Substance means: hard surface example: salt,corals,coal.

how long would it take to electroplate a flute with 28.3 g of silver (107.87 g/mol) at a constant current of 2.0 amps using AgNO3

Answers

It would take 211 hours to plate 28.3 g of silver on a flute.

The equation of the reaction;

Ag^+(aq) + e -----> Ag(s)

We know that 1 F of electricity is required to deposit 107.87 g of Ag. Also 1F = 96500 C

Now;

Since  107.87 g is deposited by 96500 C of electricity

28.3 g is deposited by  28.3 g ×  96500 C/ 107.87 g

= 25317 C

Also;

Q = It

I = current

t = time

25317 C =  2.0 amps × t

t = 25317 C /2.0 amps

t = 12658.5 seconds or 211 hours

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Answer:

It would take [tex]3.5hrs[/tex] to electroplate the flute

Explanation:

Electrolysis equation is:

[tex]Ag1+ + 1e- ------> Ag[/tex]

1 mol of Ag requires 1 mol of electron

1 mol of electron = 96485 C

So,

1 mol of Ag requires 96485 C

let us calculate mol of element deposited:

molar mass of Ag = [tex]107.87 g/mol[/tex]

number of mol of Ag, [tex]n = \frac{mass of Ag}{molar mass of Ag}[/tex]

[tex]n = \frac{28.3}{107.87}\\\\n = 0.2623 mol[/tex]

[tex]total charge = mol of element deposited * charge required for 1 mol\\\\total charge = 0.2623*9.649*10^4\\\\total charge = 2.531*10^4 C[/tex]

Therefore,

[tex]time = \frac{Q}{i}\\\\= \frac{2.531*10^4}{2}\\\\= 1.265*10^4 seconds\\\\= 3.515 hr[/tex]

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WILL GIVE BRANIEST HELP

Which procedures were followed in this lab to decrease experimental error? Check all that apply.
using only two types of seeds, both from the same source
having one student count every sprout in every quadrat
using adjacent plots of land for both groups
increasing the sample size by combining data from multiple groups
repeating the experiment indoors under growth lights
measuring plant height the same way and at the same time for both groups

Answers

Answer:

Basically A, C, D, F

Explanation:

Summarizing the answer

Using only two types of seeds, both from the same source.

What are experimental error?

Experimental error refers to the uncertainties or mistakes that can occur during scientific experiments, leading to inaccuracies in the results. These errors can arise due to a variety of reasons, such as human error, instrumental error, environmental factors, and methodological issues. The presence of experimental error can make it difficult to draw accurate conclusions from the data obtained during an experiment.

Scientists use various procedures to reduce experimental error and improve the accuracy and reliability of their results. These procedures can include:

using adjacent plots of land for both groupsincreasing the sample size by combining data from multiple groupsmeasuring plant height the same way and at the same time for both groups.

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The molecular formula for a compound with a molar mass of 180.18g that contains 40.0% carbon, 6.7% hydrogen, and 53.3% oxygen by mass is

Answers

Answer:

C6H12O6

Explanation:

First you need to find the empirical formula by the percents that they're giving you. You assume that is 100 grams and if for example you have 40% of carbon you will have 40 grams of carbon.

1.Find the number of moles of each atom

C: 40g÷12g/mol= 3.33 mol

H: 6.7g÷1g/mol= 6.7 mol

O: 53.3g÷16g/mol= 3.33 mol

You divide all ratios by the minimum ratio and you obtain the empirical formula

CH2O

Since you're given the molar mass, you now have to find the molar mass of CH2O by adding the molar mass of each element present and repeated in the compound.

CH2O= 30g/mol

Now you know that the molar mass of the molecular formula we are looking for is 180.18 g/mol, and since the empirical formula is the molecular formula in its smaller ratio, by logic you can say that:

180.18g/mol ÷ 30g/mol= 6

So, you now know that the empirical formula is being multiplied by a factor of 6 to reach a compound with a molar mass of 180.18g/mol, so you multiply each subscript of the empirical formula by 6 obtaining:

C6H12O6

What is the amplitude of the above wave?

Answers

Answer:Amplitude in a longitudinal wave

Explanation:Wave amplitude of a longitudinal wave is the distance between particles of the medium where it is compressed by the wave. Wave amplitude is determined by the energy of the disturbance that causes the wave. A wave caused by a disturbance with more energy has greater amplitude.

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Answers

Answer:

1) actual yield - 2.675 g

Theoretical yield - 4.0539 g

2)a) 41.257 g

b) 0.6857moles

c) 23 g

Explanation:

1)

Theoretical yield is the mass of the product that can obtain from the equation, it is not practical.

Theoretical yield:

Mr of SiO2 - 60

Moles of SiO2 - 2.339/60 = 0.03898 moles

Mr of SiF4 - 104

Mass of SiF4 - 0.03898 * 104 = 4.05392 g

2)

a)

mr of B2H6 - 28

Moles of B2H6 - 36.1/28 = 1.2893 moles

Mr of O2 - 32

Mass of O2 - 32 * 1.2893 = 41.257 g

b)

Moles of B2H6 - 19.2/28 = 0.6857 moles

Stoichiometry 1:1 ratio between B2H6 and H2O

So moles of H2O is 0.6857

c) moles of O2 - 2/24(r.t.p) = 0.083 moles

Mass of B2H6 - 0.083 * 28 = 2.3 g

Earth’s layers are determined by their physical properties and chemical composition. In the lesson, you learned about each of Earth’s layers, what the layers are made of, and why they are important to the Earth. In the space below, write about how the atmosphere may be classified. Would it be part of the lithosphere, or a separate layer? Explain your answer.

Answers

Answer:

The Atmosphere Is a separate layer And Also could be classified as the Closest layer

Explanation:

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A gaming computer system and additional equipment was purchased for $2,000. The system and equipment depreciate in value by 10% each year.After how many years will the computer have a value less than $1,000?A. 1B. 3C. 7D. 8 How much does it cost to repair a broken train? (Fill in the blanks) What was an effect of having so many men away from home during World War II?It permanently changed the role of women in the workforce.It opened up opportunities for minorities and women.It eliminated racial discrimination permanently.It changed American family values. Read the excerpt from "Amigo Brothers.Large posters plastered all over the walls of local shops announced the fight between Antonio Cruz and Felix Vargas as the main bout.The fight had created great interest in the neighborhood. Antonio and Felix were well liked and respected. Each had his own loyal following. Betting fever was high and ranged from a bottle of Coke to cold, hard cash on the line.Antonios fans bet with unbridled faith in his boxing skills. On the other side, Felixs admirers bet on his dynamite-packed fists.Which tone would be most appropriate for a text trailer that includes this scene?a tone of happinessa tone of sadnessa tone of anticipationa tone of desperation In a proposed business venture, a businesswoman estimates there is a 65% chance she will make $57000 and a 35% chance she will lose $67000. Determine her expected value. why does your heart rate increase when you exercise What is the relevance of osmosis to the root hair cells? 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Or, selon les mdecins, si on observe l'horaire des 35 heures au prix des tarifs qui sont actuellement appliqus, ils sont moins bien pays que les plombiers ! Je n'ai rien dire contre des plombiers bien rmunrs, mais on ne peut comparer la longueur des tudes (6 annes plus un concours pour l'internat) et la rigueurdes tudes mdicales celles qui permettent l'obtention d'un brevet professionnel !Je pense surtout que les gnralistes la campagne font des dplacements longs et souvent dans des conditions difficiles (par tous les temps) et qu'il serait normal qu'il y ait des compensations adquates. ma connaissance, la France est un des rares pays o les mdecins gnralistes rendent encore visite leurs patients au lieu de les recevoir sur rendez-vous dans leur cabinet mdical, comme c'est le cas, par exemple, aux tats-Unis o j'ai fait personnellement l'exprience d'avoir eu me dplacer avec une fivre de cheval, un cas de bronchite aigu, pour aller consulter mon mdecin.Les franais ont donc beaucoup de chance d'avoir encore des mdecins qui se dplacent domicile et ils devraient soigner leurs gnralistes, et en prendre soin comme d'une espce en voie de disparition pour assurer la continuit d'un tel luxe.Albert Salazot Courrier lectroniqueCourrier des lecteurs Journal Franais Avril 2002Questions: 1- Selon l'article, pourquoi est-ce que les toubibs (docteurs) font-ils la grve?2- Pour quelle raison, certains franais sont-ils contre la grve?3- Que font les gnralistes la campagne, qui est difficile?4- Quest-ce qui est unique propos des docteurs en France? 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