The owners of a local bakery ask for your help in improving a special yeast strain they use to make bread. They would like you to help them design experiments using RNA interference to turn off genes, to allow them to test their hypothesis that certain genes are important for the good flavors found in their bread. Of the components in the following list, which is the most important to check for in this yeast strain if you would like this project to succeed?

a. the presence of foreign double-stranded RNA
b. the presence of genes in the genome that code for RISC proteins
c. the presence of miRNA genes in the genome
d. the presence of single-stranded siRNAs within the cell

Answers

Answer 1

Answer:

b. the presence of genes in the genome that code for RISC proteins

Explanation:

The RNA interference (RNAi) mechanism is a naturally occurring pathway by which eukaryotic cells can silence specific genes both at the transcriptional (eg., by triggering DNA methylation on specific loci) and posttranscriptional (e.g., by triggering mRNA degradation) levels. This mechanism (RNAi) is widely used in molecular biology laboratories in order to silence genes and thus understand their functions. During the RNAi pathway, double-stranded RNAs (dsRNAs) are cleaved into small double-stranded RNA (dsRNA) molecules known as small interfering RNAs (siRNAs) or microRNAs (miRNAs) by an enzyme referred to as "Dicer". Subsequently, these small non-coding RNAs bind to an enzymatic complex known as RNA-induced silencing complex (RISC) that contains a ribonuclease enzyme, i.e., an enzyme that degrades RNA. Finally, the small non-coding RNA (either siRNAs or miRNAs) that are complementary to the RNA target directs the RISC complex to bind and cleave specific mRNAs, thereby silencing gene expression (in this case, by the inhibition of protein translation).


Related Questions

Freshwater plants that have adapted to very moist conditions live in which type of biome?
an estuary
coral reefs
the ocean
a wetland

Answers

Answer:

A wetland

Explanation:

All the others are salt water

Answer:

wetland

Explanation:

indicate FOUR
reasons why you think having it may enhance responsible living in your environment

Answers

Answer:

Can you please elaborate on ur question??

: d

Please elaborate on question.

What does the red at the beginning of the video represent? What does this mean about the landscape around Mount St. Helens before 1980?

Answers

Answer:

On May 18, 1980, an earthquake struck below the north face of Mount St. Helens in Washington state, triggering the largest landslide in recorded history and a major volcanic eruption that scattered ash across a dozen states

Explanation:

Carrying capacity is Group of answer choices seldom reached by marine producers and consumers because of the vast resources of the ocean. the maximum population size that a particular environment can support. fixed for most species over most of their range most of the time. the term used to describe the stress a population undergoes due to limited resources.

Answers

Answer:

the maximum population size that a particular environment can support.

Explanation:

A population can be defined as the total number of living organisms living together in a particular place and sharing certain characteristics in common.

Generally, these populations may be divided into a fraction of the population (subpopulation) based on certain factors and reasons.

Population regulation can be defined as a biological process that balances limiting factors affecting the growth of a population based on density. The factors that regulate the growth of a population are divided into two (2) main categories and these includes;

I. Density-independent factors.

II. Density-dependent factors.

Density-dependent are regulating factors such as predation, diseases, and competition that affect the size of the population of living organisms through decreasing or increasing mortality and birth rate.

Furthermore, density-independent factors do not have an increasingly greater effect as a population's density increases. Thus, its effect are reduced as a population's density increases in size.

Carrying capacity is the maximum population size that a particular environment can support. The carrying capacity of an environment is denoted by the letter k.

How does lateral gene transfer help explain the evolution of antibiotic resistance in bacteria?

Answers

Answer:

Lateral gene transfer allows the bacteria to develop resistance to antibiotics and spread it between bacterial populations. ... This process allows bacteria to transfer genes from one population into another. As a result, the next generation would evolve and develop defenses that would make them resistant to the drug.

Explanation:

Which of the following is not a type of fossil fuel?
petroleum
nuclear energy
coal
natural gas

Answers

Answer: Nuclear energy

Explanation:

Answer:

I say the answer nuclear energy!!

Explanation:

Nuclear energy is not a fossil fuel!!

An adult hemopoietic stem cell found in the bone marrow ______________________. (a) will occasionally produce epidermal cells when necessary. (b) can produce only red blood cells. (c) can undergo self-renewing divisions for the lifetime of a healthy animal. (d) will express all the same transcription factors as those found in an unfertilized egg.

Answers

Answer: (c) can undergo self-renewing divisions for the lifetime of a healthy animal.

Explanation:

The adult hematopoietic tissue or adult hematopoietic stem cells can be found in bone marrow of the animal. These cells have the ability to regenerate and renew so that the production of red blood cells remains continuous till death. Hematopoiesis can be defined as the production of cellular components of blood. The adult hematopoietic stem cells are located in the flat bones and ends of the long bones.

define the process of photosynthesis

Answers

Plants use sunlight, water, and carbon dioxide to create oxygen and energy in the form of sugar :)

Answer:

The process by which the green plants prepare their food in presence of sunlight , water and carbon dioxide is called photosynthesis.

During photosynthesis in green plants, light energy is captured and used to convert water, carbon dioxide, and minerals into oxygen and energy-rich organic compounds.

If a company genetically modifies corn to produce an odor that repels insects, which best describes how the corn was modified?​

Answers

Answer:

★ The corn was modified by water

Explanation:

Hope you have a great day :)

Describe the levels of organization found in your neighborhood from the smallest level of organization to the largest.

Answers

Answer:tissue

Explanation:

Tissues are groups of cells that have a similar structure and act together to perform a specific function. The word tissue comes from a form of an old French verb meaning “to weave”. ... Groups of tissues make up organs in the body such as the brain and heart.

Which planets are considerably larger

Answers

Answer:

Jupiter, Saturn, Uranus, and Neptune

Explanation:

Jupiter: Jupiter is the largest planet in the Solar System. With a mean diameter of 142,984 km, it is big enough to fit all the other planets (except Saturn) inside itself, and big enough to fit Earth 11.8 times over.

Please answer fast ASAP I give brainliest



How is light penetration likely to affect the distribution of plants in the lake ?

Answers

Explanation: In the open water zone (or photic zone) sunlight supports photosynthetic algae and the species that feed upon them. In the deep water zone, sunlight is not available and the food web is based on detritus entering from the littoral and photic zones.

Hope this helps!!

Plz Branliest!!

Have a great day!!

Bye!!

what are the components of the atmosphere that help organisms live on earth?
EXPLAIN and I will make you brainliest!!!

Answers

Answer: Atmospheric gases, especially carbon dioxide (CO2) and oxygen (O2), are extremely important for living organisms. Carbon dioxide and oxygen are the gases in the atmosphere that are needed for life. Plants need carbon dioxide for photosynthesis. They use sunlight to change carbon dioxide and water into food. The process releases oxygen

Explanation:

Which group of organisms would Schleiden have been most likely to study?

Answers

Answer: flower, pine tree, or cactus.

Explanation:

Answer:

A: flower,pine tree,or cactus

Explanation:

Edge 2021

Most of the energy radiated by Earth's surface at night is in the form of

Answers

Answer:

infrared rays

Explanation:

The energy that Earth receives from sunlight is balanced by an equal amount of energy radiating into space. The energy escapes in the form of thermal infrared radiation: like the energy you feel radiating from a heat lamp.

Which of the following explains the conservation of mass during cellular respiration? (4 points) a The total number of atoms when carbon dioxide and oxygen react stays the same when glucose and water are produced. b The total number of atoms when water and oxygen react stays the same when glucose and water are produced. c The total number of atoms when carbon dioxide and water react stays the same when glucose and oxygen are produced. d The total number of atoms when glucose and oxygen react stays the same when carbon dioxide and water are produced.

Answers

Answer:

D- The total number of atoms when glucose and oxygen react stays the same when carbon dioxide and water are produced.

Explanation:

The conservation of mass during cellular respiration can be explained by the fact that after cellular respiration which occurs due to the oxidation of glucose to produce carbon dioxide and water, the number of atoms on the right hand side (RHS) of the reaction remain same as of left hand side (LHS) of the reaction.

The balanced chemical equation is mentioned as under:

C₆H₁₂O₆  +     6 O₂         ->     6 CO₂       +      6H₂O    +     Energy.

(Glucose)     (Oxygen)          (Carbon            (Water)

                                              dioxide)

If we will see carefully, the number of atoms of each element on RHS & LHS of the equation are same i.e. there are 6 atoms of C, 18 atoms of O and 12 atoms of H on both the sides.

The allele W, for white fur, is dominant over allele w, for black fur. In a sample of 500 mice, 476 are white and 24 are black. Estimate the allelic frequencies in this sample assuming the population is in equilibrium.

Answers

Genetic drift: Evolution (defined as a change in allele frequencies) that occurs in ... Assume that 50% of the alleles for fur color in a population of mice are B ... Assume a population is in Hardy-Weinberg equilibrium for a given genetic ... What is the frequency of black heterozygous cattle in the population? ... White by blese.

In mouse, genes X, Y and Z are on chromosome 2. The map of genes X, Y and Z is:

X--------20mu--------Y---------------30mu---------Z.

You cross an individual with genotype XXYYZZ to an individual with genotype xxyyzz, and F1 progeny are collected. Assume interference between regions (X-Y and Y-Z) is 40%. You then perform a cross between an F1 individual and an individual with genotype xYz/xyz. Among 1000 progeny, how many of them will be of the genotype xxyyzz?

Answers

Answer:

36 individuals xxyyzz

Explanation:

Available data:

X-Y = 20MUY-Z = 30MUCross: XXYYZZ   x    xyyzzF1= XxYyZzI = 40%2nd Cross: XxYyZz  x  xxYyzzF2 = 1000 individuals

We need to know how many individuals from the F2 are going to be xxyyzz

xxyyzz genotype is the result of a double recombinant event.

First, we need to analyze the given information:

→ Distances between genes

The genetic distance result from multiplying the recombination frequency of each region by 100 and expressing it in map units (MU). So, if we have the genetic distance we can divide it by 100 and get the recombination frequency:

Let us say that region I is the distance between X and Y. And region II is the distance between Y and Z.

GD = genetic distance

RF = Recombination frequency

GD (X-Y) = RF x 100

20MU = RF x 100

20MU/100 = RF

0.2 = RF (Region I)

GD (Y-Z) = RF x 100

30MU = RF x 100

30MU/100 = RF

0.3 = RF (Region II)      

Now we have the recombination frequency of Region I and Region II.

→ Interference    

Knowing the interference value will let us know the value of the coefficient of coincidence, which will also lead us to the number of individuals xxyyzz.

I = 40%

I = 1 - CC   ⇒ CC = coefficient of coincidence

Clearing this equation gives us the value of CC

CC = 1 - I

CC = 1 - 40% = 1 - 0.4

CC = 0.6

To calculate the coefficient of coincidence, CC, we can also use the next formula:

CC = observed double recombinant frequency/expected double recombinant frequency

Now, we are interested in knowing the value of the observed double recombinant frequency.

Note:  

observed double recombinant frequency = total number of observed double recombinant individuals/total number of individuals expected double recombinant frequency = recombination frequency in region I x recombination frequency in region II.

So, to get the value of the observed double recombinant frequency, first, we need to get the value of the expected double recombinant frequency. And we can do it because we already have the recombination frequencies in both regions.

expected double recombinant frequency = recombination frequency in region I x recombination frequency in region II

- Recombination Frequency - RI = 0.2

- Recombination Frequency  - RII = 0.3

expected double recombinant frequency = 0.2 x 0.3

expected double recombinant frequency = 0.06 = EDRF

Now that we know the value of the CC and the expected DRF, we can get the observed double recombinant frequency.

CC = observed double recombinant frequency/expected double recombinant frequency

- CC = 0.6

- EDRF = 0.06

CC x expected double recombinant frequency = observed double recombinant frequency

0.6 x 0.06 = observed double recombinant frequency

0.036 = observed double recombinant frequency = ODRF

Finally, as this is a frequency, we need to multiply it by the total number of individuals in the F2 to get the number of observed double recombinant individuals.

- ODRF = 0.036

- N = 1000

Double recombinant individuals = 0.036 x 1000 = 36 individuals xxyyzz.

True or False? The skills and characteristics needed for a high-quality friendship are the same skills and characteristics needed in a committed long-term relationship or marriage.

Answers

True
Hope this help

In DNA, a sequence of three bases is a code for the placement
of a certain amino acid in a protein chain. The table shows some
amino acids with their abbreviations and DNA codes.
Which amino acid chain would be produced by the DNA base
sequence below?
C-A-A-G - T- T - A-A-A-T - T-A

Answers

G - T- T- C - A - A - T - T - T - A - T

What is happening to the [OH-] when the solution turns from pink to violet?

Answers

Answer:

the hydroxide ions are undergoing ionization and the particles are gaining / losing electrons .

Explanation:

i think it is because ::

the phenolphthalein of the solution has gone from having a pH of around 8 (pink) to being alkaline at above 10 which causes it to burn purple / violet .

Please help!! I have to turn this in like right now lol

Answers

Answer: A

explanation(from a website)

Stay calm. ...

Remove the stinger. ...

Wash the sting with soap and water.

Apply a cold pack to reduce swelling. ...

Consider taking over-the-counter pain medication.

Answer:

must be A

Explanation:

first A as first aid

and then B

1. This question may be completed independently or as a group exercise. Biological anthropologists disagree about the best way to classify the robust australopithecines. Describe the two approaches to classifying these species. Which classification do you support

Answers

Answer:

Some researchers classify Australopithecines into gracile australopithecines and robust australopithecines, while others argue that differences are enough strong to classify robust australopithecines in their own genus called 'Paranthropus'. In my opinion, it seems reasonable to obtain more fossil data in order to support this division  

Explanation:

The term Australopithecines comes from the genus Australopithecus, which are the closest known relatives of our genus Homo, and arose around three (3) million years ago. Australopithecines can be classified into two major groups called gracile australopithecines and robust australopithecines. However, robust australopithecines have also been classified in their own genus, Paranthropus, in order to distinguish them from the gracile australopithecines. The major differences between gracile and robust species are associated with facial and cranial features. Paranthropus species had a large sagittal crest, enormous cheek teeth, robust jaws, and strong jaw muscles (suggesting they were herbivores that ate tough food). However, these main features may not be conclusive evidence that supports the separation of Paranthropus in its own clade, thereby in my opinion it should be reasonable to wait until more fossils are found in order ​to correctly classify Paranthropus species as a different group.

Freshwater ecosystems can be grouped as those that are still, those that are flowing, and those that contain salt. (True or False)

Answers

Answer:

true

Explanation:

Please help and thank you I do not want my points wasted

Answers

I can give you an idea: Train the pets on what exactly to do during a natural disaster

Which of the following statements about yeasts are true?
1) Yeasts are fungi
2) Yeasts can form pseudohyphae
3) Yeasts reproduce asexually by budding
4) Yeasts are facultatively anaerobic
5) All yeasts are pathogenic
6) All yeasts are dimorphic
Select the option below that contains the numbers referring to true statements.
NO LINKS!!!

Answers

Answer:

Yeasts are fungi, that is the answer

What are four examples of learned behaviors in animals?

Answers

Answer:

Habituation. Sensitization. Classical conditioning. Operant conditioning.

Explanation:

Types of learning include habituation, sensitization, classical conditioning, operant conditioning, observational learning, play, and insight learning. One of the simplest ways that animals learn is through habituation, where animals decrease the frequency of the behavior in response to a repeated stimulus.

I’ll give extra points for who ever does this

Answers

You see this is correct because

pleaaaseeee helpp meeeeeee ​

Answers

4 is a pterodactyl!!!!!!

I WILL GIVE BRAINLIEST AND EVERYTHING IF YOU HELP ME

In order to go through Mitosis, DNA is packaged into structures known as what.


a

Enzymes

b

Chromosomes

c

Double Helix strands

d

Nucleic Strands

Answers

Answer:

b:chromosomes

Explanation:

hope you get it

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