To run a spectrophotometry experiment, begin by _______ the spectrophotometer and preparing the samples. Be sure to select the correct ________, then run a measurement on the _______ solution. Follow up by running measurements on _______ solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples.
1.
a) cleaning
b) warming up
c) unplugging
2.
a) wavelength
b) transmittance
c) absorbance
3.
a) blank
b) aqueous
c) sample
4.
a) blank
b) aqueous
c) sample

Answers

Answer 1

Answer:

b) warming up a) wavelength a) blank c) sample

Explanation:

To run a spectrophotometry experiment, begin by warming up the spectrophotometer and preparing the samples. It is important that the equipment is warmed up for at least 30 minutes before starting the measurements.

Be sure to select the correct wavelength, then run a measurement on the blank solution. The selected wavelength depends on the analyte of interest. The black solution contains the same matrix but it doesn´t contain the analyte.

Follow up by running measurements on sample solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples. The samples are those of unknown concentration that we want to determine.

Answer 2

To run a spectrophotometry experiment, begin by cleaning the spectrophotometer and preparing the samples. Be sure to select the correct wavelength, then run a measurement on the sample solution. Follow up by running measurements on aqueous solutions. Once data is collected, turn off the instrument, clean the area, and discard the samples.

Spectrophotometry is a technique used to measure the interaction of light with matter, specifically the absorption, transmission, or reflection of light by a sample. It involves the use of a spectrophotometer, an instrument that measures the intensity of light as a function of its wavelength or frequency.

In spectrophotometry, a sample is exposed to light of a specific wavelength or a range of wavelengths. The sample may absorb certain wavelengths of light, which can be detected and measured by the spectrophotometer. The amount of light absorbed is related to the concentration of the analyte in the sample, allowing for quantitative analysis.

Learn more about Spectrophotometry, here:

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Related Questions

Calculate the amount of heat required to completely sublime 55.0 g of solid dry ice CO2 at its sublimation temperature. The heat of sublimation for carbon dioxide is 32.3 kj mol

Answers

Answer:

40.4 kJ

Explanation:

Step 1: Given data

Mass of CO₂ (m): 55.0 gHeat of sublimation of CO₂ (ΔH°sub): 32.3 kJ/mol

Step 2: Calculate the moles corresponding to 55.0 g of CO₂

The molar mass of CO₂ is 44.01 g/mol.

n = 55.0 g × 1 mol/44.01 g = 1.25 mol

Step 3: Calculate the heat (Q) required to sublimate 1.25 moles of CO₂

We will use the following expression.

Q = n × ΔH°sub

Q = 1.25 mol × 32.3 kJ/mol = 40.4 kJ

How does the Big Bang theory explain why there is a lot of hydrogen and helium in the universe?

Answers

Answer:

when the explosion happened the matter fussed into elements as the electrons portions Nd notrons were compresed into the elements beacuase hydrogen has 1 nutron proton and electron surronding its core

Gamma rays have the highest frequency among all common electromagnetic radiations. It means the gamma rays have the highest energy and the longest wavelength

Answers

Answer:

yes

Explanation:

cos of high electron transfer

Based on your knowledge of factors affecting the rate of reaction, why is there a danger of explosions in places such as flour mills and coal mines where there are large quantities of powdered, combustible materials? 

Answers

The large quantities of powdered, combustible materials in silos and coal mines would equate to a higher concentration of materials and technically, a higher surface area. Coincidentally, these places often harbor higher temperatures, increasing the collision frequency. With these three factors combined, there would be an increased reaction rate and this case, a quicker rate for the combustible materials to be released in the form explosions.

aluminum chloride and bubbles of hydrogen gas are produced when metallic aluminum is placed in hydrochloride acid. what is a balanced equation for this reaction?

Answers

Answer:

AlCl3 + HCl ===> AlCl4 +H-

Which metal does not form cations of differing charges?

Answers

Transition metals

Most transition metals differ from the metals of Groups 1, 2, and 13 in that they are capable of forming more than one cation with different ionic charges. As an example, iron commonly forms two different ions

a polluted lake is 0.300 μg (micrograms) per liter of water, what is the total mass of mercury in the lake, in kilograms, if the lake has a surface area of 15.0 square miles and an average depth of 27.0 feet?

Answers

Answer:

95.9 kg

Explanation:

First we convert 15.0 mi² to m²:

15.0 mi² * ([tex]\frac{1609.34 m}{1mi}[/tex])² = 3.88x10⁷ m²

Then we convert 27.0 ft to m:

27.0 ft * [tex]\frac{0.3048m}{1ft}[/tex] = 8.23 m

Now we calculate the total volume of the lake:

3.88x10⁷ m² * 8.23 m = 3.20x10⁸ m³

Converting 3.20x10⁸ m³ to L:

3.20x10⁸ m³ * [tex]\frac{1000L}{1m^3}[/tex] = 3.20x10¹¹ L

Now we calculate the total mass of mercury in the lake, using the given concentration:

0.300 μg / L * 3.20x10¹¹ L = 9.59x10¹⁰ μg

Finally we convert μg to kg:

9.59x10¹⁰ μg * [tex]\frac{1kg}{1x10^9ug}[/tex] = 95.9 kg

A mixture of gases at 2.99 atm can You have two gases, and , at the same temperature. Determine the ratio of effusion rates of and .ists of 13.2 moles of hydrogen gas and 19.1 moles of helium gas. Determine the partial pressure of the helium gas.

Answers

Answer:

Given total pressure of the gas mixture(hydrogen and helium) is 2.99 atm

Number of moles of hydrogen is ---- 13.2 mol

Number of moles of helium is ---- 19.1 mol

Determine the partial pressure of the helium gas.

Ratio of effusion rates of the two gases.

Explanation:

According to Dalton's law of partial pressures,

partial pressure of a component gas in a mixture is:

partial pressure of a gas = total pressure x mole fraction

mole fraction of helium gas is:

[tex]mole fraction of helium gas = \frac{number of moles of helium gas}{total number of mioles} \\=>mole fraction of He= \frac{19.1mol}{(19.1+13.2)mol} \\=>mole fraction of He = 0.591\\[/tex]

Partial pressure of He gas is:

[tex]Partial pressure of He =mole fraction of He * total pressure\\ =0.591 x 2.99atm\\ =1.77atm[/tex]

Effusion rate of a gas is inversely proportional to its square root of its molecular mass.

[tex]\frac{rate of effusion of H2 gas}{rate of effusion of He gas} =\sqrt{\frac{molar mass of He gas}{molar mass of H2 gas} } \\=> \frac{rate of effusion of H2 gas}{rate of effusion of He gas}=\sqrt{\frac{4g.}{2g} } \\=>\frac{rate of effusion of H2 gas}{rate of effusion of He gas}=1.414:1[/tex]

Hence, rates of effusion of H2:He is 1.414:1.

Learning Task 1. Read each item carefully. Choose the letter of your answer.
1. Which type of soil is characterized as having the finest particles holding greater amount of water?
A. Loam
B. Clay
C. Sand
2. Which type of soil is best for planting?
A. Loam
B. Clay
C. Sand
3. How does each soil types differ?
A. Texture
B. Color
C. Both A & B
4. Which type of soil do you usually expect if the community is along the seashore?
A. Loam
B. Clay
C. Sand
5. Why is soil important to living things?
A. Forms part of the earth where animals live
B. Provides the necessary nutrients needed by plants
C. Serves as a place where people live
D. All of the above​

Answers

Answer:

1. B

2. A

3. C

4. C

5. D

Explanation:

Soil is regarded as the solid unconsolidated material of the earth crust. Soil is of three different types namely: Sandy soil, clay soil and loamy soil. These three different soil types possess different properties that distinguish them. Some of them are:

- CLAY soil is characterized as having the finest particles and can hold greater amount of water i.e. have a high water holding capacity.

- LOAMY SOIL is the best soil type for planting agricultural crops because it has the highest concentration of nutrients that suited for plant growth.

- loamy, Sandy and clay differ in how we feel when touched i.e. texture, and colour.

- SANDY soils are the kind of soils that are found in Sea shores and beaches.

- Soil is important to living things as it forms part of the earth where animals live, provides the necessary nutrients needed by plants, serves as a place where people live.

The pH for several solutions is given below. After filling in each blank with the correct number, arrange the substances mentioned at the beginning of each sentence in order from most acidic to most alkaline.
a. Black coffee has a pH of 5, therefore its H+ concentration is __________times greater than a solution with a pH of 10
b. Urine has a pH of 6, therefore its H+ concentration is ________times greater than a solution with a pH of 9.
c. Household ammonia has a pH of 11, therefore its H+ concentration is__________ times less than a solution with a pH of 7 .
d.Grapefruit juice has a pH of 3, therefore its H+ concentration is_______ times greater than a solution with a pH of 5 .
2
100
1000000
4
100000
10
3
100,000
1000
5

Answers

how

the

Way

of

it's

the Base

Look at the equation CO2 + H2 = CO + H20.

CO is a ________

reactant and a product

reactant

product​

Answers

Answer:

CO is considered as a product.

Explanation:

A general chemical equation for a combination reaction follows:

To write a chemical equation, we must follow some of the rules:

The reactants must be written on the left side of the direction arrow.

A '+' sign is written between the reactants, when more than one reactants are present.

An arrow is added after all the reactants are written in the direction where reaction is taking place. Here, the reaction is taking place in forward direction.

The products must be written on the right side of the direction arrow.

A '+' sign is written between the products, when more than one products are present.

For the given chemical equation:

are the reactants in the reaction and  are the products in the reaction.

Hence, CO is considered as a product.

Determine the Concentration of the Unknown Strong Acid
In this activity you will use the virtual lab to determine the concentration of a strong monoprotic acid. To do this, you can perform
a titration using NaOH and phenolphthalein found in the virtual lab. (Note: The concentration of the acid is between 0.025M and
2.5M so you will need to dilute the NaOH solution so that the volume to reach the endpoint is between 10 and 50 mL).
Once you have determined the concentration of the acid, please enter your answer into a form at the bottom of this page.
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Lab
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Unknown Acid Problem
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Answers

Hey baby I know it’s late but I wanted to say how are u doing

Calculate the density of CO2 at a pressure of 685.0 torr and 41.0°C .
R=0.0821 (L*atm)/(mol *K)

Answers

T=41+273=314 k

M=(12)+(16×2)=44g/mol

d=PM/RT

d=685×44/0.0821×314

d=1169.15 g/L

what is the enthalpy of the overall chemical reaction

Answers

Answer:

For a chemical reaction, the enthalpy of reaction (ΔHrxn) is the difference in enthalpy between products and reactants; the units of ΔHrxn are kilojoules per mole

The sloth is able to camouflage by moving really slowly and
A. changing colors
B. hanging upside down
D. having fur that is a similar color to tree barks with algae growing on it

Answers

Answer:

D. having fur that is a similar color to tree barks with algae growing on it

Explanation:

Choice A doesnt make sense because sloths do not change colors, and hanging upside down would not protect you from prey or blend in. D matches the description of a sloth.

Answer:

Having fur that is a similar color to tree barks with algae growing on it.

Explanation:

2. Which of the following best represents the nucleus in a model of an atom?
a positively charged nucleus consisting of protons and neutrons
O a positively charged nucleus consisting of electrons and neutrons
a neutral nucleus consisting of protons and neutrons
a neutral nucleus consisting of electrons and neutrons

Answers

Answer:

A positively charged nucleus consisting of protons and neutrons

Explanation:

The atomic nucleus is a positively charged region located at the core of an atom that consists of positively charged protons and neutral neutrons while the negatively charged electrons make up the outer cloud (electrons are therefore not contained in the nucleus).

Balance the equation by ion electron method

Answers

Answer:i believe you are to decompose the formula (i think)

Guys i need a long inforation about....
"Formation of colloids"
Please and thank you ​

Answers

Answer:

Colloids

There are two basic methods of forming a colloid: reduction of larger particles to colloidal size, and condensation of smaller particles (e.g., molecules) into colloidal particles. Some substances (e.g., gelatin or glue) are easily dispersed (in the proper solvent) to form a colloid; this spontaneous dispersion is called peptization. A metal can be dispersed by evaporating it in an electric arc; if the electrodes are immersed in water, colloidal particles of the metal form as the metal vapor cools. A solid (e.g., paint pigment) can be reduced to colloidal particles in a colloid mill, a mechanical device that uses a shearing force to break apart the larger particles. An emulsion is often prepared by homogenization, usually with the addition of an emulsifying agent. The above methods involve breaking down a larger substance into colloidal particles. Condensation of smaller particles to form a colloid usually involves chemical reactions—typically displacement, hydrolysis, or oxidation and reduction.

Which is most likely the reason the U.S government made such an investment

Answers

Answer:

The U.S government made such an investment because they're most likely to reduce harmful emissions.

iodide (KI). Identify the correct products of this
reaction. (Note that NO3? is the nitrate ion, a
polyatomic ion.)
AgNO3 + KI – ?
AgK + INO3

Agl + KNO3
Ag + K + I + NO3
COMPLETE
What type of reaction is this?

Answers

Answer:

AgNO3 + KI → Agl + KNO3. Double-replacement reaction

Explanation:

Based on solubility rules, Silver, Ag produce an insoluble salt in presence of halides (Fluorides F-, Chlorides Cl-, Iodides, I-, and bromides Br-). That means the mixture of AgNO3 (Ag⁺ ions) with KI (I- ions) produce AgI as insoluble salt.

The reaction is:

AgNO3 + KI → Agl + KNO3

And this is a double-replacement reaction where the cations exchange of anion to produce 2 new compounds.

Answer:

AgI + KNO3

Explanation:

double replacement

What is the concentration of a solution that is made by diluting 50.0 mL of a 0.40 M NaCl solution to a final volume of 1000.0 mL?​

Answers

Answer:

The correct answer is 0.020 M.

Explanation:

To solve this problem, we can use the equation M1V1 = M2V2, where M represents the molarity of the solution and V represents the volume of solution.  Since we are given that the original solution is 50 mL and 0.40 M, these values are V1 and M1, respectively.  The solution after dilution has a volume of 1000 mL, so this value is V2.  We are solving for the molarity after dilution, which represents M2.

If we plug in the values specified above, we get the following:

M1V1 = M2V2

(0.40 M)(50.0 mL) = (M2)(1000.0 mL)

Solving for M2, we get:

M2 = 0.020 M

Notice that our answer has 2 significant figures because 0.40 has 2 significant figures, the least of any values given in the problem.

Therefore, the answer is 0.020 M.

Hope this helps!

Give the following for SO2 and BrF5:
(a) Number of domains on central atom (b) Domain geometry (c) Molecular geometry (d) Hybridization of central atom

Answers

Answer:

The given molecules are SO2 and BrF5.

Explanation:

Consider the molecule SO2:

The central atom is S.

The number of domains on S in this molecule is three.

Domain geometry is trigonal planar.

But there is a lone pair on the central atom.

So, according to VSEPR theory,

the molecular geometry becomes bent or V-shape.

Hybridization on the central atom is

[tex]sp^{2}[/tex].

Consider the molecule BrF5:

The central atom is Br.

The number of domains on the central atom is six.

Domain geometry is octahedral.

But the central atom has a lone pair of electrons.

So, the molecular geometry becomes square pyramidal.

The hybridization of the central atom is [tex]sp^{3} d^{2}[/tex].

The shapes of SO2 and BrF5 are shown below:

HELP PLS THIS IS SO HARD AHHHHHHH

Answers

1. The box like figure in the given image is the [BATTERY SOURCE] from where the current drawn into the circuit.

2. A string connecting positive terminal of battery to the bulb is an [ELECTRIC WIRE] through which current flows in the circuit.

3. A bubble like object in the circuit is a [BULB] which lights up when current moves through the circuit.

4. A component connected to the negative terminal of batter source is a [SWITCH].

The answer are uppercase.

I hope this helps c:

why Hydroxide ion is less strong base than hydride ion​

Answers

Answer:

The hydride ion,H−is a stronger base, so it will abstract a proton from the water. As the water will give protons, so water will work as an acid and an acid-base reaction will take place. Hydride ions get protons from water and form hydrogen gas and hydroxide ions.

Sodium hydride is the chemical compound with the empirical formula NaH. This alkali metal hydride is primarily used as a strong yet combustible base in organic synthesis. NaH is a saline (salt-like) hydride, composed of Na+ and H− ions, in contrast to molecular hydrides such as borane, methane, ammonia, and water.

the other answer is correct, replying so you can give them brainliest asap! :)

Consider the dehydration of 2-propanol in sulfuric acid: There is a scheme of a reaction where CH3 CH CH3 with an OH group attached to the second carbon atom dehydrated in the presence of H2SO4. Draw the structure for the product of the dehydration of 2-propanol.

Answers

Answer:

CH2=CH-CH3

Explanation:

One of the ways of preparing alkenes is by dehydration of alcohols using sulphuric acid. The sulphuric acid merely acts as a catalyst in the process.

It begins with the protonation of the -OH group. This is followed by loss of water molecule since water is a good leaving group.

Loss of a proton now completes the mechanism and regenerates the acid catalyst.

The mechanism of dehydration of 2-propanol as well as the product formed in the process is shown in the image attached to this answer.


Lemon juice has a pH of 2. What is the hydrogen ion concentration of lemon juice?

Answers

pH = −log [H+] = − log [5.4 × 10−3] ≈ 2.27 or 2.3.
or basically 2

what are the most common measurements used to describe gases in chemistry

Answers

tempature, volume , amount , pressure

Four quantities must be known for a complete physical description of a sample of a gas: temperature, volume, amount, and pressure. ... The height of the mercury column is proportional to barometric pressure, which is often reported in units of millimeters of mercury (mmHg), also called torr.

only some particles split up into smaller particles

Answers

Answer:

Everything around you can be broken down into smaller particles called atoms. The particles of one substance are all the same and different substances are made up of different particles.

Explanation:

How much energy is produced when 13.1 g of tin reacts with 2.715 g of N2 ?
I.
3 Sn + 2 N2-----------Sn3N4 + 632 KJ
Hint change grams to moles first.
1 mole Sn= 119g
1 mole N2= 28 g

Answers

Answer:

23.2 kJ of energy are released by the reaction.

Explanation:

Hello there!

In this case, according to the given information, it turns out firstly necessary for us to calculate the moles of both tin and nitrogen and the produced moles of Sn3N4 product by each reactant as shown below:

[tex]13.1gSn*\frac{1molSn}{119gSn} *\frac{1molSn_3N_4}{3molSn} =0.0367molSn_3N_4\\\\2.715gN_2*\frac{1molN_2}{28gN_2} *\frac{1molSn_3N_4}{2molN_2} =0.0485molSn_3N_4[/tex]

Thus, since 13.1 grams of tin produce the fewest moles of Sn3N4 product, we infer tin is the limiting reactant, and the correct produced energy, due to this reaction is:

[tex]E=632\frac{kJ}{mol\ rxn}*\frac{1mol\ rxn}{1molSn_3N_4}*0.0367mol Sn_3N_4\\\\E=23.2kJ[/tex]

Regards!

molar mass of Beryllium

Answers

Answer:

The molar mass of Beryllium is 9.012182 u

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