Which factors make sequencing by the sanger chain-termination method possible? select all that apply.

Answers

Answer 1

Gel electrophoresis can be used to differentiate the sizes of duplex nucleic acid molecules. A duplex molecule can be created by joining complementary separate nucleic acid sequences.

What is duplex RNA or DNA?

The duplex structure supports the Watson-Crick base pairing model for GNA containing antiparallel strands. The right-handed helical ribbon that is wrapped around the helix axis to create a big hollow core is how one can define the duplex secondary structure, which is different from the canonical A- & B-form nucleic acids.

How are RNA duplexes created?

One of the most biologic systems is the duplex synthesis of nucleic acids. During replication, RNA primers create a duplex with parental strand of DNA. (1) A brief stretch of duplex must be formed during translation in order to recognize codons against anticodons.

To know more about Duplex nucleic acid visit;

https://brainly.com/question/14147877

#SPJ4


Related Questions

This model shows a strand of dna. Identify how information for specifying the traits of an organism is carried in the dna.

Answers

The nitrogen bases in DNA serve as a code for the order of amino acids that make up proteins, demonstrating that the DNA contains information describing the characteristics of an organism.

How does DNA carry information for describing an organism's characteristics?

DNA stores genetic information in a linear arrangement of nucleotides. The two complementary strands of nucleotides in each DNA molecule are held together to form a double helix by a hydrogen bond between the G-C and A-T base pairs.

What part of DNA influences an organism's traits?

Gene. a section of the DNA molecule (a series of nucleotides) responsible for encoding a certain protein and determining an individual's phenotypic characteristics. The fundamental building block of heredity in a living thing is a gene.

To know more about DNA strand visit:-

https://brainly.com/question/11503015

#SPJ4

_____ is an injury that is the result of overuse, overstretching, or forcible stretching of a muscle beyond its functional capacity. It sometimes involves a tendon or ligament.

Answers

Chronic strains are an injury brought on by overusing, overstretching, or forcibly reaching a muscle beyond its range of motion. A tendon or ligament may occasionally be involved.

Usually entails a strained or torn ligament, is a joint injury?

A sprain is a stretching or tearing of the ligaments, the strong bands of fibrous tissue in your joints that link two bones together.

The knee's semilunar cartilages are involved in which injury?

Additional cartilage strips, known as semilunar cartilages, support the knee joint on both sides. Meniscal tears or splits are among the most frequent knee injuries. extreme impact or twisting, particularly when engaging in weight-bearing activities.

To know more about Chronic strains visit:-

https://brainly.com/question/401728

A parent that is heterozygous for a trait is crossed with a parent that is homozygous dominant for the trait, as shown in the Punnett square.



Which genotype belongs in space B?

PP
Pp
ppp
Ppp

Answers

Pp is the right answer

Answer:

Pp

Explanation:)

How does the probability of an earthquake occurring evolve with time, in between two different earthquakes?.

Answers

The factors that determine the likelihood of an earthquake are history, strain accumulation, and the rate at which strain accumulates in a rock.

An earthquake occurs when two blocks of earth suddenly slip past each other. The fault or fault plane is the surface where they slip. Earthquakes are caused by sudden movement along fault lines within the Earth. The movement releases accumulated 'elastic strain' energy in the form of seismic waves, which travel through the Earth and cause the ground to shake.

Within the next 30 years, there is a 60% chance of an earthquake measuring magnitude 6.7, a 46% chance of an earthquake measuring magnitude 7, and a 31% chance of an earthquake measuring magnitude 7.5. A transform boundary causes a fault between two lithosphere plates, which slide past one another. This motion neither creates nor destroys crust and will result in earthquakes but no volcanoes.

To learn more about earthquakes, here

https://brainly.com/question/14239677

#SPJ4

organisms, such as algae and bacteria, that drift with ocean currents are known as . group of answer choices benthos organisms none of these nekton plankton biomass previousnext

Answers

Organisms, such as algae and bacteria, that drift with ocean currents are known to be Plankton.

So, the correct option is D.

Organisms transported by tides and currents are known as plankton or marine drifters. The word "plankton" is derived from the Greek word meaning "wanderer" or "drifter." If an organism is transported by currents and tides but is unable to swim well enough to resist these forces, it is referred to as plankton.

Numerous aquatic food webs are built on phytoplankton. They provide food for a variety of aquatic animals in a healthy habitat. In that they have chlorophyll and need sunlight to survive and develop, phytoplankton often referred to as microalgae, are like terrestrial plants in this regard. Drift is the name given to the slow-moving ocean current. They are impacted by wind belts and atmospheric circulation.

Learn to know more about Plankton on

https://brainly.com/question/1120604

#SPJ4

what is the name of the hormone producing cells in the middle of this endocrine organ, indicated by the arrow?

Answers

Answer:

Pituitary. The pituitary gland is located below the brain. Usually no larger than a pea, the gland controls many functions of the other endocrine glands.

Explanation:

You didnt include the image with the arrow so im not sure

the physiological and behavioral effects of amphetamine are most similar to those of:

Answers

its behavioral and physiological effects are similar to those of cocaine

In pavlov’s classical conditioning experiments, the neutral stimulus that comes to elicit a conditioned response is known as the.

Answers

In Pavlov's experiments, the neutral stimulus was the bell, the unconditioned stimulus was the food, and the conditioned stimulus was the salivation that occurred due to the bell.

Define Pavlov's experiments.

By accident, classical conditioning was discovered. When Pavlov studied the digestion of dogs, he found that the canines' physical reactions to food evolved over time. At first, the dogs would only slobber when their food was offered to them. But later, before they got their food, they made a little salivation. They were drooling when they heard sounds that were always present before the food arrived, such the sound of an approaching food cart, according to Pavlov.

In order to prove his theory, Pavlov set up an experiment where he rang a bell right before feeding the dogs. At first, the bells had little effect on the dogs.

To know more about Pavlov's experiments, visit:

https://brainly.com/question/4486274

#SPJ4

kirima is a native eskimo who lives in one of the coldest regions in the world. what type of adipose tissue is most effective at helping her to maintain warm body temperatures during the coldest parts of the year?

Answers

Brown fat, also known as brown adipose tissue, is a type of body fat that keeps you warm in the winter. Brown fat also stores energy and aids in calorie burn.

What is Brown fat ?

Brown fat, also known as brown adipose tissue, is a type of body fat that activates when you are cold. Brown fat generates heat, which helps to keep your body temperature stable in cold weather. Brown fat has far more mitochondria than white fat.

Brown fat burns calories by producing heat just before your body begins to shiver. It also aids in the regulation of sugar and fat metabolism.

Turmeric, green tea, chilli peppers, fish oil, resveratrol, berberine, and cinnamon are examples of brown fat foods and compounds that increase activity or induce the browning of white fat. Cold exposure and moderate exercise are two other ways to activate brown fat.

Exposing your body to cool, even cold, temperatures may aid in the recruitment of more brown fat cells. According to some studies, just 2 hours of daily exposure to temperatures around 66°F (19°C) may be enough to turn recruitable fat brown. Consider taking a cold shower or an ice bath.

To learn more about Brown fat refer :

https://brainly.com/question/13097739

#SPJ4

PLEASE URGENT !!
Which level of ecological organization is each statement describing?

1. One fish
2. A school of fish
3. All of the living organisms in a pond
4. All of the living and non living things in a pond
5. All of the living and non-living things that interact in the same area
6. One single living thing
7. All of the different populations that live in the same area
8. A group of organisms of the same species that live in the same area
9. All parts of earth that have living organisms

Answer options:

A. Population
B. Community
C. Biosphere
D. Ecosystem
E.Organism

Answers

The level of ecological organization each statement describes is like below.

A. Population: All of the living and non-living things that interact in the same area.

B. Community: All of the different populations that live in the same area.

C. Biosphere: One fish; A school of fish and All of the living organisms in a pond.

D. Ecosystem: All of the living and nonliving things in a pond; One single living thing and All parts of the earth that have living organisms.

E.Organism: A group of organisms of the same species that live in the same area.

In order to comprehend the circumstances surrounding the emergence, development, and demise of organizations, organizational ecology makes use of ideas from biology, economics, and sociology as well as statistical analysis.

Researchers in the field of ecology focus on five major levels: organism, population, community, ecosystem, and biosphere. These levels are sometimes studied separately and other times they overlap.

To learn more about Ecological organization visit: https://brainly.com/question/1323075

#SPJ1

which part of the flower is labeled with an x

Answers

Answer:

Ovary

Explanation:

The ovary is a pair of female type glands in which the eggs start form and the female hormone's estrogen and progesterone are made produced.

" I hoped I help you : - ) "

cell membrane short description

Answers

Answer:

All cells have a cell membrane, also known as a plasma membrane, which separates the interior of the cell from the external environment. A semipermeable lipid bilayer makes up the cell membrane. The movement of materials into and out of the cell is controlled by the cell membrane.

Explanation:

An organism’s traits are determined by the specific combination of inherited _____.

Answers

An organism’s traits are determined by the specific combination of inherited genes.

Traits are characteristics or attributes of an organism that might be expressed by means of genes and/or prompted by way of the surroundings. trends include bodily attributes of an organism along with hair color, leaf form, size, and so forth., and behavioral traits, inclusive of birds nesting.

A trait, as related to genetics, is a specific feature of a man or woman. traits may be determined by genes, environmental factors, or by means of an aggregate of each. traits can be qualitative (together with eyeshade) or quantitative (inclusive of peak or blood pressure).

Traits are characteristics or attributes of an organism that might be expressed by genes and/or motivated by the surroundings. traits encompass physical attributes of an organism consisting of hair color, leaf form, size, and many others., and behavioral characteristics, which include chicken nesting.

Learn more about  organism’s traits  here:

https://brainly.com/question/22265986

#SPJ4

What is the difference between Cell Differentiation and Mitosis?

Answers

Answer:

the difference is how to do it and how it's done

Answer:

In order for a cell to differentiate, it will express specific genes. Once a cell has differentiated, it loses its ability to undergo mitosis (see below). Cell division involves the splitting of a cell Mitosis is a form of cell division: this is the process by which cells split to form new cells.

HELPPP PLS ASAP!! 2 ATTACHED 50 POINTS!
PS.have a lovely day

Answers

I am having difficulties so I screen shotted it.

why do memory cells respond more strongly during a secondary immune response than naive lymphocytes do in a primary immune response?

Answers

Memory cells respond more strongly during a secondary immune response than naive lymphocytes do in a primary immune response  they have already gone through clonal selection and proliferation when antigen was encountered previously.

Immunological memory is a completely unique belongings of the immune system as it may “keep” facts approximately a stimulus and may mount an powerful reaction when the stimulus is encountered once more. This response – a secondary immune reaction – is faster and stronger than the primary reaction.

In immunology, a memory B mobile (MBC) is a form of B lymphocyte that forms part of the adaptive immune device. these cells expand inside germinal facilities of the secondary lymphoid organs. reminiscence B cells flow into in the blood flow in a quiescent nation, sometimes for decades.

however, a small part of lengthy-lived T cells nonetheless remains for rapid response upon pathogen re-publicity. This type of cells is called memory T cells. because memory T cells were skilled to apprehend specific antigens, they may cause a quicker and stronger immune reaction after encountering the identical antigen.

Learn more about Memory cells  here:

https://brainly.com/question/15196473

#SPJ4

If you crossed flies heterozygous for the cy allele, what phenotypes would you find in the adult f1 generation?.

Answers

If you crossed flies heterozygous for the cy allele, the phenotypes would both wild type and curly wings be present in the F1 generation.

It is 1st filial generation obtained by crossing two different parents. The parents are quite distinct: one is homozygous dominant for both traits, and the other is homozygous recessive for both traits. All such offspring show dominant characteristics for both traits.

The F1 generation refers to the first filial generation.

When you're heterozygous for a specific gene, it means you have two different versions of that gene

To learn more about heterozygous, click here:

https://brainly.com/question/1626661

#SPJ4

a human cell placed into a hypertonic solution is likely to a human cell placed into a hypertonic solution is likely to lose water by osmosis. burst as a result of osmosis. remain unchanged. increase in size.

Answers

A cell placed into a hypertonic solution will shrivel and die by a process known as plasmolysis.

What is hypertonic solution?

A hypertonic solution is defined as any external solution with a high solute content but a low water concentration as compared to bodily fluids. The net movement of water in a hypertonic solution is out of the body and into the solution. If the solute concentration outside the cell is lower than the concentration inside the cell, the solution is hypotonic to the cell. If the concentration of solutes in the solution is greater than that inside the cell, the solution is hypertonic to the cell. The influx of water entering the cell causes the cell to increase volume and expand.

To know more about hypertonic solution,

https://brainly.com/question/29309024

#SPJ4

For the red onion skin experiment, in what cellular structure is red onion pigment stored and you were observing shrinkage?.

Answers

There are no chloroplasts in onions since they do not photosynthesize. The central vacuole houses the purple pigment (anthocyanin) that gives red onions their distinctive color, making it simple to identify.

What is Plasmolysis?

The goal of this experiment is to better understand plasmolysis and deplasmolysis in red onion cells by observing water transport into and out of cells. Plasmolysis takes place when the environment of epidermal red onion cells is changed by the addition of concentrated sucrose solution, which has less water potential than onion cells. Deplasmolysis is the name given to the process if it is reversed. By putting pure water in the onion cells' exterior environment, deplasmolysis can be seen. The protoplast inside the cell enlarges to its original size during deplasmolysis as water is absorbed into the cells. The mechanisms in different plant cells can be compared using these observations.

To know more about Plasmolysis, visit:

https://brainly.com/question/28099561

#SPJ4

Which of the following does not contribute to the rate of weathering?

climate

fossils

rock type

mineral composition

Answers

Answer:

It's rock type..............

Nearly all stimuli destined for the cerebral cortex must first pass through the ________.

Answers

Nearly all stimuli destined for the cerebral cortex must first pass through the thalamus.

The outer layer of the brain is the cerebral cortex. Its surface has many folds, giving it a rumpled look. The folds are made up of many deep grooves called sulci and raised areas called gyri.

The cerebral cortex is made from 4 lobes: frontal lobe, parietal lobe, temporal lobe, and occipital lobe.

The main function of the cerebrum is to control the voluntary muscle movements of the body. The cerebral cortex is primarily involved in consciousness.

The thalamus is an egg-fashioned shape withinside the middle of the brain. It is known as the relay station for all incoming motor (movement) and sensory information (auditory, gustatory, visual, and tactile (but not olfactory).

The thalamus is a small structure in the brain just above the brainstem, between the cerebral cortex and midbrain, and has extensive neural connections to both. The principal characteristic of the thalamus is to transmit motor and sensory alerts to the cerebral cortex.

Learn more about thalamus from:

https://brainly.com/question/6330373

#SPJ4

How does osmosis occur. Explain.

Answers

Answer:

Osmosis is the movement of water from areas of high concentrations to lower concentrations across a semi permeable membrane. It occurs over these membranes in cells of the body alllowing water to move into and out of them. Due to this nature the cell membrane is described as being partially (or selectively) permeable. Osmosis is a special kind of diffusion involving water molecules. It occurs when two solutions are separated by a partially permeable membrane.

Explanation:

Answer:

Osmosis occurs when you have membrane with unequal concentrations of water molecules on either side. Water molecules will diffuse across the membrane until equilibrium is reached.

Explanation:

which of the following factors does not affect the daily urine volume? the environmental temperature the respiratory rate the relative humidity the body temperature all of these factors affect the daily urine volume.

Answers

A correct answer is an option (E). all of these factors affect the daily urine volume.

An average adult excretes between 750 and 2000 ml of urine each day.

The eating of specific foods is one of the many variables that might affect how much pee a person generates.

the volume of liquid taken in.

the volume of food eaten.

the volume of liquid lost as a result of breathing and sweating.

medical issues and certain medicines

the blood sugar level and Blood Pressure

Salt and Alcohol intake

Exercise (sweating)

Environment temperature.

To learn more about what is urine please click on the given link: https://brainly.com/question/29347560

#SPJ4

Hemizygosity would most likely be associated with which of the following?
a. Sex linked inheritance
b. Incomplete Dominance
c. Trihybrid crosses
d. Sex inherited inheritance
e. Dihybrid crosses

Answers

Answer:

A. Sex linked inheritance

Explanation:

what major component of mucins directly provides a polyanionic surface to which defensins and siga bind?

Answers

A polyanionic surfaces Sialic acid is directly provided by mucins' main constituent.

What makes DNA a polyanion?

RNA and DNA are both polyanions. They are simply polymer of negatively charged molecules, to put it fancy. This is due to the hydroxyl group in the sugar-phosphate backbone, which typically carry a charge around negative three.

Is DNA digitalizable?

The method of transferring and decoding binary code to and from synthetic DNA strands is known as DNA digital data storage. Although DNA has a huge amount of potential as a storage device due to its high density, its use in practical applications is now greatly restricted due to its exorbitant cost and extremely sluggish read and write speeds.

To know more about Polyanionic visit:

https://brainly.com/question/23191608

#SPJ4

The nurse is working with a patient with diagnosed secondary varicose veins. The nurse suspects which patient history is the cause of the varicose veins?.

Answers

The nurse suspects which patient history is the cause of the Tibia fracture.

The tibia, sometimes known as the shinbone, is the most commonly broken long bone in the body. A tibial shaft fracture can occur anywhere along the length of the bone between the knee and the ankle.

A large force is usually necessary for this type of fractured leg to occur. Car accidents, for example, frequently result in tibial shaft fractures. Along with the tibia, the smaller bone in the lower leg (fibula) is frequently shattered.

The sort of fracture that develops is determined by the force that breaks the tibia. Bone fragments can be completely aligned (stable fracture) or not (displaced fracture). The skin around the fracture may be unaffected depending on whether it is open or closed (open fracture).

Learn more about Tibia fracture at

https://brainly.com/question/14524362?referrer=searchResults

#SPJ4

Cells that develop into neurons and glia originate in the​

a. ​ventricular zone.
b. ​radial glia.
c. ​forebrain.
d. ​spinal cord.

Answers

Cells that develop into neurons and glia originate in the​​ ventricular zone.

Ventricular zone (VZ) is a transient embryonic layer of tissue containing neural stem cells, mainly radial glial cells, of the central nervous system (CNS).

Ventricular zone gives rise to neuroblasts and globalists (future supporting cells) which migrate into the intermediate zone form two collections of cells separated by a groove called the sulcus limitans. Ventricular system of the brain functions to enhance support to surrounding tissues having cerebrospinal fluid (CSF), which are produced in the choroid plexus tissue lining many of the ventricles.

To learn more about Ventricular zone  , here

brainly.com/question/10977527

#SPJ4

the frequency of crossing over between any two linked genes will be which of the following? a. proportional to the distance between them b. higher if they are recessive c. higher if they are dominant d. the same as if they were not linked

Answers

Any two connected genes will cross over more frequently in proportion to their distance from one another.

What is the highest frequency of recombination between two genes in terms of percentage?

Therefore, the maximum recombination frequency that can be seen is 50%, which is a sign of either loci on distinct chromosomes or loci that are far distant from one another on the same chromosome.

Is recombination always 50% of the time?

The frequency of recombination is particularly low when genes on a chromosome are near to one another. Recombination occurs 50% of the time when genes are spread out across two or more chromosomes.

To know more about gene visit:-

https://brainly.com/question/8832859

#SPJ4

Plants are adapted to life on land. Can you identify the function of each adaptation? Pollen, Egg, Vascular System, Cuticle,
Leaves, Lignin, Shoot System

Answers

The requirements of life on land over time fostered the development of plants that were more tolerant to the sun's withering rays, better at conserving water, as well as able to reproduce without it.

A water-repellent integument, stomata to control water evaporation, specialized cells that can provide strength and rigidity against gravity, structure and firm to collect sunlight, variation of haploid as well as diploid generations, as well as sexual organs are just a few of the structures that plants have developed to adjust to life on land.

An important part of reducing the unavoidable effects of climate change at local as well as regional levels is the adaptability of land-use patterns; one example would be the adaptation of watershed land-use patterns to lessen the effects of climate change on such a region's hydrology.

To know more about plants

https://brainly.com/question/22167412

#SPJ4

On bacteria how are pilus and flagella different and similar?

Answers

Flagella are helical appendages that protrude through the cell membrane and are long and whip-like. Pili are hollow filamentous extensions that emerge from the cell's surface.

Flagella are whip-like and pili are hair-like. Flagella are extended from the interior cell body while pili extend from the cell surface. pili are mostly found in bacteria.

The pilus is a hair-like structure associated with bacterial adhesion and related to bacterial colonization and infection.

To learn more about Flagella , here

brainly.com/question/905839

#SPJ1

Other Questions
a nurse is caring for a client with an injury to the central nervous system. when caring for a client with a spinal cord insult that is slowing transmission of the motor neurons, in what would the nurse anticipate a delayed reaction? An airplane at an altitude of 3000 feet descends at a rate of 500 feet per mile. help needed asap !!!! A line has a slope of -9 and includes the points (-9, n) and (-10, 5). What is the value of n?n= Why is photosynthesis and cellular respiration considered a cycle? In an experiment, you test whether the number of times you rub a cloth against a balloon changes how long it will stick against the wall. You start by rubbing the balloon 10 times with the cloth and stick it to the wall. You repeat this three times and record your results in a table.ObservationNumber of Times Balloon is Rubbed by Cloth Trial #1 Trial #2 Trial #310 6 seconds 20 seconds 20 secondsWhat statement best reflects why you should do repeated trials in an experiment? Rubbing the balloon against a cloth 10 times produces very different results each time it is repeated. The first trial is right because it is done with new materials that give the best results. The second and third trials were wrong because they were very different from the first trial. You would make a wrong conclusion if it were based on the first trial in the experiment. An elevator can carry 24 adults or 40 children at one time during the course of a day the elevator Carries a full passenger load 74 times if all the passengers were children how many people would the elevator Carry than if all the passengers were adults Which of the following are characteristics of the graph of the linear parentfunction?Check all that apply.A. It goes through the origin.B. It has a slope of 1.C. It is a parabola.D. It is a straight line. Wendy solved a problem with her calculator, and she got this answer:2.35E7Which expressions are equivalent to the answer she got? Select all that apply.2.35 7395.823,500,00016.452.3572.35 x 10 when a company has market power, it is in its market. not a price-taker not able to impact the market equilibrium price a producer of nondifferentiated products one of many small companies Guided Problem Solving You have a class photo that is 10 in. long and 8 in. wide. If you want to enlarge your photo to be 15 in. long, how wide will the photo be?Understand the Problem You know the dimensions of the original photo and the length of the enlarged photo. Find the width of the enlarged photo.Make a Plan Draw the figures and label their sides. (a)Bill invests Rs 75000 for 10 years at a rate of 6 % per year simple interst. Calulate the total interest he will receive.(b) Harry invests Rs 24 000 at a rate of 7 % per annum at simple interest.After how many months will Ravi gain Rs 1260 as interst? How is Reading Like a Writer similar to and/or different from the way(s) you read for other classes? If the nominal wage is $30 in 2011 and the CPI is 202 in 2011, then the real wage in 1982-1984 dollarsA) is $29.00.B) is $1.48.C) is $14.85.D) is $30.E) cannot be calculated without the past year wage rate. A responsibility center is best described as? a. any part of an organization that has responsibility for optimizing customer service and satisfaction b. any financial sub-unit within an organization that has the responsibility to account for the performance of other organizational sub units. c. any part of an organization whose manager has control over and is accountable for cost profit or investments d. any part of an organization that has responsibility for managing product and service quality Use a table of values to graph the function (x) = 2 x . Select the correct graph below. HOW DID ROOSEVELT USE THE BUFFALO SOLDIERS TO HIS BENEFIT?Help pls Ill mark brainliest!! Tasha plants seeds in clay pots. She has 40 seeds to plant. She plants 6 seeds in each pot. How many seeds does Tasha have left over? "My Escape from Slavery" by Frederick Douglass HELP ME WILL GIVE BRIANLIEST!!How does the historical setting of the work affect the main character's actions and emotions?My free life began on the third of September, 1838. On the morning of the fourth of that month, after an anxious and most perilous but safe journey, I found myself in the big city of New York, a FREE MANone more added to the mighty throng which, like the confused waves of the troubled sea, surged to and fro between the lofty walls of Broadway. Though dazzled with the wonders which met me on every hand, my thoughts could not be much withdrawn from my strange situation. For the moment, the dreams of my youth and the hopes of my manhood were completely fulfilled. The bonds that had held me to "old master" were broken. No man now had a right to call me his slave or assert mastery over me. I was in the rough and tumble of an outdoor world, to take my chance with the rest of its busy number.I have often been asked how I felt when first I found myself on free soil. There is scarcely anything in my experience about which I could not give a more satisfactory answer. A new world had opened upon me. If life is more than breath and the "quick round of blood," I lived more in that one day than in a year of my slave life. It was a time of joyous excitement which words can but tamely describe. In a letter written to a friend soon after reaching New York, I said: "I felt as one might feel upon escape from a den of hungry lions." Anguish and grief, like darkness and rain, may be depicted; but gladness and joy, like the rainbow, defy the skill of pen or pencil. During ten or fifteen years I had been, as it were, dragging a heavy chain which no strength of mine could break; I was not only a slave, but a slave for life. I might become a husband, a father, an aged man, but through all, from birth to death, from the cradle to the grave, I had felt myself doomed. All efforts I had previously made to secure my freedom had not only failed, but had seemed only to rivet my fetters (fasten my chains) the more firmly, and to render my escape more difficult. Baffled, entangled, and discouraged, I had at times asked myself the question, "May not my condition after all be God's work, and ordered for a wise purpose, and if so, is not submission my duty?" A contest had in fact been going on in my mind for a long time, between the clear consciousness of right and the plausible (reasonable) make-shifts of theology and superstition. The one held me an abject (hopeless) slavea prisoner for life, punished for some transgression in which I had no lot nor part; and the other counseled me to manly endeavor to secure my freedom. This contest was now ended; my chains were broken, and the victory brought me unspeakable joy. What is the role of the majority leader of the House?