A method which is the best choice to insert DNA into a human cell for use in long-term gene expression studies is: A. stable transfection.
What is genetic engineering?Genetic engineering simply refers to a high-tech process through which scientists remove (delete), replace, or insert a deoxyribonucleic acid (DNA) code into the cells of a living organism, so as to improve on both its physical and chemical conditions or cure (correct) a genetic disorder in some cases.
What is stable transfection?Stable transfection can be defined as a method which is used in genetic engineering for the insertion of deoxyribonucleic acid (DNA) into the cell of a living organism such as humans, especially for use in permanent (long-term) gene expression studies.
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Speed in decision-making, speed in reducing cycle-times, speed in operations, and speed in continuous improvement. AI in the supply chain is here to stay and make waves in the years to come.
According to Gartner, supply chain organizations expect the level of machine automation in their supply chain processes to double in the next five years. At the same time, global spending on IIoT Platforms is predicted to grow from $1.67B in 2018 to $12.44B in 2024, attaining a 40% compound annual growth rate (CAGR) in seven years.
In today’s connected digital world, maximizing productivity by reducing uncertainties is the top priority across industries. Mounting expectations of supersonic speed and efficiencies between suppliers and business partners of all types further underscores the need for the industry to leverage the prowess of the Artificial Intelligence (AI) in supply chains and logistics
AI in supply chains is helping to deliver the powerful optimization capabilities required for more accurate capacity planning, improved productivity, high quality, lower costs, and greater output, all while fostering safer working conditions.
When faced with a pandemic like COVID-19, establishing a good understanding of the impact on supply chains and contingency plans can help manufacturing companies deal with uncertainties in the right way.
Given the scenario above, discuss how the incorporation of AI can assist struggling businesses to make a comeback with their supply chain efficiencies in the forefront?
Determining a decision, acquiring data, and weighing potential solutions are all steps in the decision-making process.By gathering pertinent data and clarifying options, a step-by-step decision-making process can assist you in making more careful, considered selections.
How can I speed up my decision-making?
Speed in operations, speed in decision-making, speed in cutting cycle times, and speed in continual improvement.In the years to come, AI in the supply chain will continue to make waves.Supply chain firms anticipate a two-fold increase in the amount of machine automation in their supply chain activities over the next five years, according to GartnerClarify Accountability. It sounds basic, but having clearly accountability is crucial to getting work done effectively. ... Get the Right Data in Front of Decision Makers. Give Yourself a Decision-Making Framework. .. Check for Biases. ..Take a Deep Breath.Quick decision-makers bear the danger of making bad choices that could hurt both them and their coworkers in the future.They frequently cause stress for team members who are not as open to change because they prefer to like it.Maximizing productivity by lowering uncertainty is the top priority across sectors in today's connected digital world.The need for the sector to take advantage of artificial intelligence's (AI) prowess in supply chains and logistics is further underscored by the rise in expectations of supersonic speed and efficiencies among suppliers and business partners of all kinds.Supply chain AI is assisting in providing the potent optimization capabilities necessary for more precise capacity planning, higher efficiency, high quality, lower costs, and greater output, all while promoting safer working conditions. Manufacturing businesses may cope with uncertainty in the proper way when faced with a pandemic like COVID-19 by developing a thorough grasp of the impact on supply chains and contingency plans.
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difference between physical and social environment in tabular form?
A physical habitat for humans is one that includes elements like soil, air, and water. While The collective beliefs, practices, and behaviors of a society make up its social environment.
Define social environment.The immediate physical and social setting in which people live, or in which anything occurs or develops, is referred to as the social environment, social context, sociocultural context, or milieu. It comprises the social groups and organizations an individual interacts with as well as the culture in which they were raised or currently reside.
Define physical environment.People live, learn, work, and play in physical environments. People's physical surroundings are influenced by the air they breathe, the water they drink, the homes they reside in, and the transportation they use to go to and from work and school. Our ability to live long, healthy lives—as well as that of our families and neighbors—can be impacted by our physical surroundings.
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What's the first step in all DNA isolation methods?
Question 7 options:
Filtration
Purification
Lysis
Centrifugation
Lysis of DNA is the first step in all DNA isolation methods.
What is known as DNA isolation?In medical science, the DNA isolation methods tells about the method designed to extract a DNA from the cortex and, then purify the nucleic acid therein to remove any inhibitors of the amplification process.
In this process, there are 3 steps involved in a DNA extraction which includes a lysis, precipitation and purification. In the lysis, the nucleus and the cell are broken open and this releases DNA. The process of lysis involves a mechanical disruption and uses enzymes and detergents like Proteinase K to dissolve the cellular proteins and free DNA.
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How does the business of Roman gladiators compare to modern men and women who fight for a living?
Answer:
Much like today’s modern sporting heroes, gladiators had a lot of physical attraction. Just as women today frequent events to see their favorite crush, ancient women would attend gladiatorial games thrilled to see their favorite fighter.
True or False: Every year the island imported enough slaves to equal half of the white population of the island.
It is true that Every year the island imported enough slaves to equal half of the white population of the island.
What is the reason why some of the slaves are been imported to the land?The reason why some of the slaves are been imported to the land is that they can be used to do their labor activities.
It should be noted that the majority of this slaves are been used on the farm to make plantation as well as the harvesting of crops on the land, some of these slaves are been used to carry out trading activities since they will be providing a free labor activities to their owner.
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Explain five roles chief can play to promote unity and national integration
Chief plays a significant role in promoting national integrity and unity by working for the welfare of the country according to the Indian Constitution.
Some roles that the chief can play are as follows:
Providing life to citizens which are free from discrimination against some communities.Respect and treat everyone equally, but according to their work or tribe.Providing education and equal opportunities to everyone.Facilitating inter-tribe or inter-caste marriages.Promoting and respecting everyone's culture.National Integration and unity mean that the country is unified despite being diverse. There is a feeling of brotherhood and trust between all citizens.
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As the owner of NR Studios you have been asked to create a
playlist of 10 songs that best represents the novel that you have
just read. Create a playlist for songs that reflects the themes
and ideas in the novel.
Answer:
Think about what theme each song on your play list performs, and from there decide what theme it's going to have.
Explanation:
Explanation:
What novel is it? Is NR Studios a real or make believe place?
Farmers in Mexico make up the
wealthiest part of the economy.
True
False
Please help me please
Answer:
1) The chain link fence is 280m long
2) The distance between garden 1 and 2 is 130m
Explanation:
In three to five sentences, analyze how and why the effect climate has on settlement patterns has changed. PLEASE HELP MEEEEEEEEEEEEE
Answer:
Climate change is likely to disproportionately affect socially vulnerable populations already inclined to migrate, thereby increasing rates of migration.
Explanation:
Increasing sea levels, the frequency of extreme weather events like floods, droughts, and storms, and the spread of tropical illnesses are all effects of rising global temperatures which affects the settlement pattern seriously.
What is climate change?Long-term changes in temperature and weather patterns are referred to as climate change. These changes could be caused by natural processes, such oscillations in the solar cycle.
But since the 1800s, human activities—primarily the combustion of fossil fuels like coal, oil, and gas—have been the primary cause of climate change.
The use of fossil fuels like coal, gas, and oil is the primary contributor to climate change. When fossil fuels are used, carbon dioxide is released into the atmosphere, warming the earth.
Burning fossil fuels to provide power and heat accounts for a sizable portion of world emissions.
Burning coal, oil, or gas still supplies the majority of the world's electricity, which produces carbon dioxide and nitrous oxide, two potent greenhouse gases that cover the planet and trap the sun's heat.
Human health is already being impacted by climate change. Life is at stake when weather and climatic trends change. One of the most lethal meteorological phenomena is heat.
Hurricanes are becoming more intense and wetter as ocean temperatures rise, which can result in both direct and indirect fatalities.
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What steps can be taken communicate more effectively and personally with these groups
At the point when colleagues can communicate successfully with one another, they will actually want to effectively team up well and work together to arrive at a shared objective more. Great communication inside a group considers the better trade of thoughts.
What is Communication?Communication is typically characterized as the transmission of data. The term can likewise allude just to the message communicated or to the field of request concentrating on such transmissions.
A significant qualification is between verbal communication, which occurs using a language, and non-verbal communication, for instance, through motions or looks. Models of communication attempt to give a definite clarification of the various advances and elements included.
Therefore A powerful model is given by Claude Shannon and Warren Weaver, who contend that informative inspiration prompts the source to create a message, which is then encoded and sent.
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why there was a need to eliminate the Articles of Confederation and create the US Constitution.
Answer:
The Articles established a weak central government and placed most powers in the hands of the states. Under the Articles, the US economy faltered, since the central government lacked the power to enforce tax laws or regulate commerce.
The _______ cortex is responsible for interpreting sound information.
Question 11 options:
olfactory
somatosensory
auditory
visual
To allow consumers to purchase products directly from its website, a business needs a/an _______.
Question 18 options:
e-commerce platform
electric vehicle
root cause
design system
With an aim of allowing the consumers to purchase products directly from its website, a business needs an e-commerce platform.
What is an e-commerce platform?Basically, an e-commerce entails the act of maintaining relationships and conducting business transactions that include selling information, services, and goods by means of computer telecommunications networks. It can also be defined as trading of goods and services over the Internet in which such economic activity takes place on.
The set of commerce consists of business-to-consumer, business-to-business commerce as well as, the internal organizational transactions that support those activities.
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how does altitude affect the temperature in different latitudes?
Answer:
Explanation:
Due to the extent of latitude, the amount of solar energy received varies according to the different differences. So latitudes look like climate that way. At higher altitudes, temperature rises. That's why the hills are cooler in summer.
• Which medium did you like better, and why?
Some critics have linked the Tea Party movement to
O negative television advertising.
O Internet blogs.
O network news broadcasts.
O newspaper opinion pages.
O conservative talk radio.
Some critics have linked the Tea Party movement to conservative talk radio. Beginning in 2009, the Republican Party in America saw a financially conservative political movement known as the Tea Party.
Members of the movement demanded lower taxes, as well as a reduction in government spending to cut the federal budget deficit and national debt. The movement rejected government-sponsored universal healthcare and backed small-government ideals. According to some accounts, the Tea Party movement is a well-known constitutional movement made up of libertarian, right-wing populist, and conservative action.
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What was at the heart of cities in early civilzations? A.people's homes B. trading center C. temples and other religious build D. Schools and libraries
The heart of the cities in early civilisation was people's homes as all
It is February 2000. The world appears to be closing and becoming increasingly insular because of what may be called a global pandemic. You are a foreign policy adviser to the government of a medium-sized West African country. To date, your country has not identified any pandemic related cases or suffered any related deaths. However, with the death rate increasing in several neighbouring countries you will soon need to advise the government in adopting a stance in relation to this disease
As a person that has to offer the advises to the government in relation to the pandemic, one of the first advise I would give is for the government to stop accepting the people from other nations that have these diseases in our nation. Next I would advise that the nation starts to help to create the vaccines that would be able to combat the disease early before it spreads into ours nation.
What is a pandemic?This is the term that is used to refer to the occurrence of a disease that has become an issue all over a given nation or in the whole part of the world.
These are the diseases that have the ability of going viral. For a nation that is still safe from its spread, one of the smartest thing to do would be to shut down its borders and flights so as to keep the people secure from passengers that already have this issues.
Also the government can also help in the research and the development that would assist to combat the issue that is being faced.
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What is an example of an interpretation?
They appeared happy.
It smelled like cinnamon.
It sounded like birds.
It tasted like soap.
Answer:
(A) They appeared happy.
Explanation:
Correct on my quiz.
"They appeared happy" is an example of an interpretation.
An interpretation is the process of giving a statement or observation meaning. For instance, the phrase "They seemed happy" implies that one can infer from people's behavior that they showed positive or contented signs. Similar to this saying something "smelled like cinnamon" implies comparing a certain scent to the well known scent of cinnamon.
The phrase "it sounded like birds" refers to hearing a sound that is similar to the calls and chirps that birds frequently make. Last but not least, "It tasted like soap" refers to identifying a flavor that has a distinct soapy flavor, possibly denoting an unpleasant or soapy taste experience. Interpretation is the process of understanding the intended meaning behind statements or observations by making sense of sensory cues, contextual information and personal experiences.
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why NYC is the cultural and tourism capital of the world
NYC (New York City) is the cultural and tourism capital of the world because of its culture, excellent economy and a top operational choice of many reputed companies, New York have migrations of many foreigners.
New York City is status's as a cultural capital of the world is build, in part on the affluence of culture, museums and art galleries that provide every visitor the chance to adore in a wide amaze of works from all over the world. There are as many as 170 museums and over 1,000 art galleries.
New York City has been expressed as the cultural capital of the world. The culture of New York is considered in its size and national diversity.
More than 800 languages are spoken in New York, creating it the most culturally diverse city in the world. Many American cultural movements first appeared in the city.
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Danielle is a mean preschool teacher and wants to teach her students to fear the school principal, who comes to check on their classroom at the same time every day. she attempts to use classical conditioning by turning off the classroom lights (US) immediately before the principal comes ibn (NS). The resulting darkness causes the children to be naturally fearful (UR). after a few weeks of trying this, Danielle is disappointed that the children do not seem to be developing a fear of the principal.
1. Classical conditioning is learning through an association Ivan Pavlov proved this through an experiment where the ringing of the bell was associated with giving the food.
AS per Pavlov’s experiment
Before the experiment when the bell was not associated
UCS (Unconditioned stimulus) =food
UCR (Unconditioned response) =Salivation
During conditioning
UCS = food, and bell
UCR= Salivation
when the dog is conditioned
CS conditioned stimulus = Bell
CR conditioned response = salivation
In this experiment
US = darkness, UR = fear (of darkness)
US = darkness, CS = principal
CS = principal, CR = fear (of principal)
1. The children show the tendency of fear with darkness
US=darkness and UR fear (of darkness) is already present in the children (assuming that all the children fear darkness)
2. We know the NS (neutral stimulus) is paired with UR fear (of darkness)
3. in the second step during conditioning the teacher is putting the lights off to create the darkness and by doing this the children are conditioned to fear.
US =darkness
CS= fear of the principal
3. In the third step, the association is established between, (after conditioning the principal is conditioned stimulus CS)
CS (principal) and the CR fear of the principal
After a few weeks, Danielle noticed that the children do not respond the way they were responding before because they had adapted to darkness and did not show any fear.
B. In order to create classical conditioning properly, she should have put the lights off just immediately before the principal comes in. In this case, the coming of the principal (beginning he was an NS) is the sign that the lights will be put on that means with the coming of the principal the children are relieved.
She should have put the lights off at the same time the principal enters.
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On January 1, 2021, Dreamworld Co. began construction of a new warehouse. The building was finished and ready for use on September 30, 2022. Expenditures on the project were as follows: January 1, 2021 $ 313,000 September 1, 2021 $ 465,000 December 31, 2021 $ 465,000 March 31, 2022 $ 465,000 September 30, 2022 $ 313,000 Dreamworld had $5,500,000 in 15% bonds outstanding through both years. The average accumulated expenditures for 2022 by the end of the construction period was:
If on January 1, 2021, Dreamworld Co. began construction of a new warehouse. The average accumulated expenditures for 2022 by the end of the construction period was: $468,000.
Average accumulated expendituresAverage accumulated expenditures:
January 1, 2021 $313,000 x 12/12 = $313,000
September 1, 2021 $465,000 x 4/12 = $155,000
December 31, 2021 $465,000 x 0/12 = $0
Total $1,243,000 $468,000
Average accumulated expenditures = $313,000 + $155,000 + $0
Average accumulated expenditures = $468,000
Therefore If on January 1, 2021, Dreamworld Co. began construction of a new warehouse. The average accumulated expenditures for 2022 by the end of the construction period was: $468,000.
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How does your being male or famale influence your family's and peer's expectations from you
Answer:
The world have defined gender roles that have been around forever. However, times are changing. We are in the middle of letting go of traditional gender roles. Being female is no longer just to be a housewife. NowThe world have defined gender roles that have been around forever. However, times are changing. We are in the middle of letting go of traditional gender roles. Being female is no longer just to be a housewife. Now expectation are to be a strong shaker and mover. Like the old commercial use to say “I can bring home the bacon and fry it up in a pan cause I’m a woman!” We are expected to do it all with a boundless supply of energy.
Personal genetic testing services, such as 23andMe, can be used to develop an individual's genetic profile. Which biotechnology method is used in this process?
Question 9 options:
Genetic modification
Recombinant DNA
DNA synthesis
DNA sequencing
The biotechnology method which is used to develop an individual's genetic profile is: D. DNA sequencing.
What is DNA?DNA is an abbreviation for deoxyribonucleic acid and it can be defined as a group of molecules that are found within cells and they are saddled with the responsibility of storing genetic information in all living organisms.
What is DNA sequencing?DNA sequencing can be defined as a general biotechnology technique that is typically used in the laboratory to determine the genetic profile of a living organism.
In this context, we can reasonably infer and logically deduce that DNA sequencing can be used to determine the exact sequence of bases or nucleotides in a deoxyribonucleic acid (DNA) molecule.
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Compare and contrast the ideologies of Sparta and Greece.
Their system of government was one of their key points in common. There was an assembly in Sparta and Athens, whose members were chosen by the populace.
What connections and contrasts exist between Sparta and Athens?Athens is the capital of Greece. One of the most important things they had in common was their form of government. Both Sparta and Athens had assemblies, the members of which were chosen by the people. Sparta was ruled by two kings who held office until their demise or removal. Athens was ruled by archons, who were elected regularly.
What characteristics do Sparta and Athens share?Athens realized they couldn't defeat Sparta's strong army, but they had the strength of a naval unit that Sparta lacked. The two cultures had the trait of being intellectuals, which united them. They valued individuals while honoring their gods. They were both Greek city-states to start with. Although they appear to be in opposition to one another, they both exhibit various facets of Greek civilization, including beauty, intellectualism, militarism, and order. Finally, in their cooperation against the Persians, they were both on the same side.
What two things separate Sparta from Athens?Sparta was a closed society, whereas Athens was an open one. Sparta was dominated by a small group of people, while Athens was democratic. There were numerous differences. Athens and Sparta fought each other in 431 BCE.
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Which entity determines the appropriate amount of compensation for human subjects in clinical trials at a research institution?
Question 15 options:
Institutional review board
FDA
GDP
ALCOA
Institutional review board determines the appropriate amount of compensation for human subjects in clinical trials at a research institution.
Under FDA policies, an Institutional evaluate Board is group that has been officially exact to study and monitor biomedical research regarding human topics. According with FDA rules, an IRB has the authority to approve, require changes in (to at ease approval), or disapprove research. Clinical trials conducted inside the European Union are held responsible by way of independent Ethics Committees (IECs). Within the united states of America, Institutional overview boards (IRBs) have oversight and need to abide with the aid of America food and Drug management (FDA) regulations. An Institutional Evaluation Board (IRB) is a committee made of individuals who've education in clinical regions, people who've understanding and training in non-clinical areas, and participants of the community who may additionally represent folks who might participate as subjects in studies research.
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WHAT ARE THE DEVELOPMENTS ON THE SCIENCE OF MAKING MAPS ??
The old science continues to change as a result of
Modern prosthetics are often made using _______.
Question 20 options:
wood
steel
copper
carbon fibers
______ information practices is a general term for a set of standards governing the collection and use of personal data and addressing issues of privacy and accuracy.
Fair information practices is a general term for a set of standards governing the collection and use of personal data and addressing issues of privacy and accuracy.
FIP (Fair Information Practices) is general term for a set of standards governing the collection and use of personal data and addressing issues of privacy and accuracy.
What is Fair Information Practices?The fair information practice principles of the US Federal Trade Commission are rules that express generally recognized ideas about fair information practice in an electronic economy.
The FTC's investigation into how internet firms gather and utilize personal information and safeguards to ensure that practice is fair and offers adequate information privacy protection led to the development of the FTC Fair Information Practice Principles.
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