You are attempting to carry out a transposon mutagenesis experiment using the pRL27 system. The Donor strain you use was giving to you by a lab mate who has been culturing it continuously for several months. When you set up your control plates, you find that the Donor strain grows poorly on Luria DAP Agar. Your transformation efficiency is very poor for this attempt at mutageneis. What likely happened to the Donor strain

Answers

Answer 1

The most likely happened to the donor strain is that the transposon is active in the donor resulting in the gradual accumulation of mutations. The accumulation of mutations is promoted by the insertion of the transposon.

A donor strain is a bacterial strain that contains a mobilizable fragment of DNA called plasmid.

These strains (donor strains) exploit the transference properties of a conjugative plasmid to modify a particular recipient strain.

Transposons, also known as jumping genes, are mobile genetic elements that alter the genome of an organism by jumping and thus inserting them into genetic sequences (i.e., into protein-coding sequences).

In this case, the presence of a transposon in the donor strain affected the expression of many genes required for the donor strain in order to grow into Luria DAP Agar.

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Related Questions

Group of different tissues working together to do a job

Answers

Answer:

organ

Explanation:

cell tissue organ organ system organism

The answer is an organ

The concentration of sodium on the inside of the cell is 20 mM. The outside of the cell has a sodium concentration of 115 mM. Which mechanism of ion uptake would best move sodium from the inside of the cell to the outside? osmosis diffusion passive transport active transport.

Answers

Answer:

It's active transport

Explanation:

The mechanism of ion uptake that would best move sodium from the inside of the cell to the outside is active transport.

What do you mean by Ion uptake?

Ion uptake may be defined as the process of movement of ions from one side of the membrane to the other.

Active transport helps in the movement of ions against the concentration gradient. It also requires energy. The active transport of small molecules or ions across a cell membrane is generally carried out by transport proteins that are found in the membrane.

Therefore, the mechanism of ion uptake that would best move sodium from the inside of the cell to the outside is active transport.

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FIRST ONE TO ANSWER GETS BRAINLEST ANSWER How does the skull's structure help it perform its function?

a. The skull has marrow in it which produces blood cells

b. The skull has sharp teeth which are used in eating

c. The skull is hard which protects the brain from being injured

d. The skull is flexible which allows the brain to grow

Answers

Answer:

c. The skull is hard which protects the brain from being injured

Explanation:

Answer:

The one that makes sense is C.) "The skull is hard which protects the brain from being injured." here's why:

Explanation:

The skull performs vital functions. Mainly, it supports and protects the head's soft tissues. The baby's skull is soft but as the bones developed, the bones of the skull form a tough, fibrous membrane and slowly fuse to form a single skull. The mandible is the only bone that separates from other parts of the skull. The skull or known as the cranium in the medical world is a bone structure of the head. It supports and protects the face and the brain. The adult skull has a total of 22 individual bones. They don’t move and united into a single unit. The skull performs vital functions. Mainly, it supports and protects the head’s soft tissues. The baby's skull is soft but as the bones developed, the bones of the skull form a tough, fibrous membrane and slowly fuse to form a single skull. The mandible is the only bone that separates from other parts of the skull. So, as our cranium grow it gets tough enough to protect the brain from injury!

hopefully I helped! have a great day!

what molecule that is created during photosynthesis is stored by the plant as energy for later use?

Answers

Glucose.

................

more science for you guys to help me with lolz

Answers

Answer:

Hi. Good evening.

Explanation:

The part labelled A is the cell membrane and the correct option to choose is the first one.

It serves as a passage to remove metabolic wastes in both cell X and cell Y.

Hope I'm right. Please remember brainliest.

Secretory IgA is best described as _____.
A. an opsonizing antibody that facilitates uptake by M cells through Fc receptors
B. a monomeric IgA that neutralizes antigen effectively at mucosal surfaces
C. an inflammatory immunoglobulin that stimulates the chemotaxis of neutrophils into mucosal surfaces
D. a complement-activating immunoglobulin that causes destruction of invasive microflora through the membrane-attack complex
E. a non-inflammatory immunoglobulin that restricts the passage of antigens across mucosal surfaces

Answers

Answer:

d.

Explanation:

...

Blood flow to the right side of the heart muscle decreases due to constriction of the __________, and decreased blood flow to the heart tissue can result in

Answers

right coronary artery; results in ischemia

The picture shows a model of a cell.

Answers

Answer:

What is showing is called a Chloroplast. They help the plant capture sunlight energy. So 'B' is the answer.

Explanation:

Answer:

B's your answer.To use energy from sunlight to make sugars

when oxygen is unavailable during heavy exercise what process do muscle cells use for energy generation?

Answers

Answer:

anerobic resperation

Explanation:

The purpose of the foot of a molluscs is:

Answers

Answer:

Foot of a molluscs is used for locomotion and anchorage, and varies in shape and function, depending on the type of mollusk under study.

Joanna was chewing gum and talking to her friend when suddenly she swallowed her gum. Which phase of swallowing was most likely involved in the accidental swallowing of her gum

Answers

The phase of swallowing that was most likely involved in the accidental swallowing of her gum is the pharyngeal phase.

There are different phases of swallowing is divided into three, namely:

oral phase

pharyngeal phase and

esophageal phase

The oral phase is voluntary while the pharyngeal and the esophageal phase is involuntary.

The pharyngeal phase involves the movement of the bolus of food from the oropharynx into the esophagus.

Since the Joanna accidentally swallowed her gum, it is an involuntary action of the pharyngeal phase of swallowing.

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What factors affect a person's daily energy requirements?​

Answers

Answer:

Gender, age and body weight are the main determinants of total energy expenditure. Thus, energy requirements are presented separately for each gender and various age groups, and are expressed both as energy units per day and energy per kilogram of body weight.

Explanation:

Pls mark as brainliest answer if it helps you

Answer: 1 people 2 sleep 3 relationship(s)

Explanation:

the people that person is most commonly with has an affect on their endergy because if the people are able to stay up all night the person has less energy which leads to sleep if the person loses sleep they wont have energy and the brain wont function as fast and wont be able to enjoy time with friends and family which leads to relation ships which are created and monitered by the brain if person A is always near person B person A and B will have a relationship called friend ship if brother A is and sister A are twins they will most likely try to do things together or destroy any bonds they have with eachothers friends.

17
Describe how temperatures above 35 °C most likely affect the structure
and function (be specific!) of starch synthase in the particular strain.

Answers

Starch synthase has an optimum temperature of 35°c. If we differ from the optimum temperature, the enzyme’s active site will start to denature, distorting it’s structure. As a result, it will not be able to catalyse the synthesis of compounds in the body.

Describe how the diaphragm and the rib cage and lungs move during inhalation and
exhalation.


this is science pls help

Answers

Answer:

During inhalation, the diaphragm contracts and pulls downwards, the ribcage expands, allowing oxygen to be sucked in through negative pressure, which causes the lungs to inflate.

During exhalation, the diaphragm and ribcage relax, returning to their normal positions, allowing the oxygen out of the body, thus deflating the lungs.

Match each step of the scientific method on the left with the statement it describes on the right

(5,6 and 7)

Answers

Answer:

6 and 7

Explanation:

I don't know I need points

QUESTION 8
A cell has 14 chromosomes. How many chromosomes will it have after it undergoes cytokinesis in mitosis?
OA) 7
B) 28
OC) 14
D)2
E) none

Answers

Answer:

C) 14

Explanation:

There would be 2 daughter cells, but each would have 14 chromosomes

can anyone match these definitions?​

Answers

1. E
2. F
3. A
4. D
5. B
6. C

Have a lovely Friday. :)

A study is conducted to evaluate the effect of competition between foxes and coyotes for a limited food source. Which level of ecology is being studied

Answers

Answer:

Community ecology!

Explanation:

I’m just did this assignment and got it right <3

A study is conducted to evaluate the effect of competition between foxes and coyotes for a limited food source. The level of ecology being studied is community ecology. The correct option is d.

What is community ecology?

The ecosystem is the fundamental unit for maintaining life, although it extends beyond species living in a single location. The recycling of resources between organisms and their environments, between organic and inorganic forms, is essential to life.

Community ecology is the study of the structure and function of communities, which are assemblages of interacting populations of species living in a certain area or habitat.

A community in ecology is a group or association of populations of two or more different species that live in the same geographical area and at the same time, also known as a biogenesis.

Therefore, the correct option is d, community ecology.

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The question is incomplete. Your most probably complete question is given below:

molecular ecology

organismal ecology

population ecology

community ecology

Two noncellular agents, smaller than viruses, are infectious RNA strands called ___ and infectious proteins called ___. prions; viroids prions; phages viroids; phages viriods; prions prions; capsomeres

Answers

Answer:

A. prions; viroids

Explanation:

If two organisms of different species share more similar DNA sequences with each other than with other species, we can conclude that ________.

Answers

If two organisms of different species share more similar DNA sequences with each other than with other species, we can conclude that these two species are very closely related.

Anatomy and embryology, molecular biology, biogeography, and fossils all provide evidence for large-scale evolution (macroevolution).Different species with comparable architecture may be homologous (shared by ancestry) or analogous (shared due to similar selective pressures).The similarity of life's molecules is evidence that it shared an ancestor. The connections between different species can be discovered by comparing DNA sequences.Biogeography, the study of the geographical distribution of organisms, provides information on the origin and evolution of potential species.The presence of extinct species in the past and evidence of long-term evolutionary changes are both shown by fossils.

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What must happen to a chromosome for it to become chromatin?

Answers

Answer:

Chromatin condensation begins during prophase (2) and chromosomes become visible. Chromosomes remain condensed throughout the various stages of mitosis (2-5). ... However, when eukaryotic cells are not dividing — a stage called interphase — the chromatin within their chromosomes is less tightly packed.

Explanation:

The chromosomes will decondense in telophase back into chromatin

16. Which organelle may have allowed early eukaryotes to make food and produce oxygen? (a) the Golgi apparatus (b) the central vacuole (c) the plastids

Answers

Answer:

the plastids

Explanation:

Answer:  

The plastids are the organelles that compose the cells of plants and algae. These contain chloroplasts, which allow them to capture energy from sunlight. If they are ever removed, these cells will eventually lose their ability to photosynthesize food without help from another organism - they have lost an essential organelle. Without the presence of this organelle, eukaryotes may not have been able to use sunlight as a mechanism for producing oxygen or making nutrients such as glucose available for consumption.

The organelle that may have allowed early eukaryotes to make food and produce oxygen is the plastid. The correct option is c.

What is plastid?

The organelles that make up the cells of plants and algae are called plastids. These have chloroplasts, which enable them to absorb solar energy. These frequently include several kinds of pigments that can alter the color of the cell as well as pigments that are employed in photosynthesis.

These cells will eventually lose their capacity to photosynthesize food without the assistance of another creature if they are ever taken out since they have lost an important organelle.

Eukaryotes might not have been able to exploit sunlight to produce oxygen or make nutrients like glucose available for consumption without the presence of this organelle.

Therefore, the correct option is (c) the plastids.

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PLEASE HELP ME IM TIMED!

Answers

Answer:

#3 Proteins

Explanation:

"There are four classes of macromolecules (polysaccharides or carbohydrates, triglycerides or lipids, polypeptides or proteins, and nucleic acids such as DNA & RNA). Carbohydrates and lipids are made of only carbon, hydrogen, and oxygen (CHO). Proteins are made of carbon, hydrogen, oxygen, and nitrogen (CHON)."

The correct answer is carbohydrates

NEED IT ASAP!!! Which statement about the specific immune response is FALSE?
Clonal selection creates short-lived effector cells to destroy the specific antigen.
Clonal selection produces memory cells that are long-lived and can recognize the antigen in the future.
It takes about two weeks for memory cells to respond to an antigen.
The maximum response from effector cells takes about two weeks after the first time the body has been exposed to an antigen.
Memory cells divide to produce effector cells when they are exposed to their specific antigen.

Answers

Answer:

The answer is: Clonal selection produces memory cells that are long-lived and can recognize the antigen in the future.

Explanation:

The ______ (the cell body of a neuron) gives rise to branch-like processes called ______, which are the primary sites for receiving signals from other neurons.Multiple choice question.neurosoma, dendrites

Answers

Answer:

Neurosoma

Dendrites

Explanation:

Where are materials stored in single celled organisms?

Answers

Answer: Ribosomes most of the time.

Explanation:

4. According to the data in Tables, which planet in the solar system has the greatest​

Answers

what table?

Explanation:

g. What is the function of CU? ​

Answers

Answer:

to give education ,to make all the people educated ,to make society good

The control unit (CU) is a component of a computer's central processing unit (CPU) that directs operation of the processor. It tells the computer's memory, arithmetic/logic unit and input and output devices how to respond to a program's instructions

Black fur(B) in guinea pigs is dominant over white fur(b). Find the probability of a white offspring in a cross: Bb x bb. ​

Answers

Answer:

50%

Explanation:

the probability of white offspring is fifty percent

help or no branlest :(​

Answers

Answer:

B is the correct answer

Explanation:

The skeletal and muscular system  was designed to support our body's structure. Therefor making it the best fit answer.

Answer:

Skeletal and muscular systems

Explanation:

.

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