An acid-fast staining method was performed on a bacterial smear using the dyes carbol fuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present?

a. No acid-fast bacteria are present on the slide
b. Methylene blue was not used
c. Carbolfuchsin was not used
d. Acid-alcohol was not used

Answers

Answer 1

Answer: Only blue cells where present because No acid-fast bacteria are present on the slide. The correct option is A.

Explanation:

Acid fast staining method also called Ziehl-Neelsen (Zn) technique is used in the laboratory to stain samples suspected to contain Mycobacterium species. The Mycobacterium species which includes the M. tuberculosis, M. ulcerans, and M. leprae, do not stain well by the Gram technique, which is a common routine stain in the laboratory. This is because of waxy coating on its cell surface primarily due to the presence of mycolic acid.

The acid fast stain consists of:

--> A primary dye which is the carbol

fuchsin

--> A decolorizer which is the acid alcohol

--> A counter stain which is the methylene blue.

In it mechanism of action on the bacterial smear, when the primary dye is applied,the whole cell will appear red. But after decolorizing with acid alcohol, only acid fast bacteria will retain the red colour of carbol

fuchsin while the red dye from the background cells are removed. Then the counterstain (methylene blue) is applied which is picked up by non-acid-fast bacteria making the cells appear blue under the microscope.

Therefore when the bacteria smear is viewed under the microscope and only blue cells are seen, it means No acid-fast bacteria are present on the slide.


Related Questions

Differentiate between deforestation and desertification

Answers

Answer: deforestation = cutting down of trees in large scale due to which it causes soil erosion. desertification = a process by which fertile land become desert ,typically as a result for drought, deforestation etc. Deforestation is defined as the cutting down or clearing of trees from a wooded area. ... If trees are removed, the area can become much warmer and drier, which may result in desertification, which is a transformation of once fertile land into desert.

Explanation:

What body parts work together to form the digestive system?

Answers

Answer: The main organs that make up the digestive system (in order of their function) are the mouth, esophagus, stomach, small intestine, large intestine, rectum and anus. Helping them along the way are the pancreas, gall bladder and liver.

Explanation:

What is chrosome ? (Definition in own words )

Answers

Answer:

A structure found inside the nucleus of a cell. A chromosome is made up of proteins and DNA

Explanation:

:)

Answer:

a chromosome is a long DNA molecule with part or all of the genetic material of an organism. most eukaryotic chromosomes include packaging proteins called histones which aided by chaperone proteins bind to and condense the DNA molecule to maintain its integrity.

PLEASE HELP !! ILL GIVE BRAINLIEST *EXTRA 40 POINTS* DONT SKIP :(( .!

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the answer is C!! when plates move and uplift each other mountains and such, like the himalayas form

PLSSS HELPPPP ME GUYSSSSSSSSSS ​

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Regulating body temperature

Which is a likely response to this rising
demand?
O New distribution systems will be used to improve delivery of the wood to Cillian.
O The trees will be regulated to maintain the availability of the wood.
O New processes will be developed to locate the trees more efficiently.
O The wood will be used as quickly as possible while it is available.

Answers

Answer:

New processes will be developed to locate the trees more efficiently.

What is the difference between an Eukaryotic cell and a Prokaryotic Cell?

Answers

Answer:

i d k

Explanation:

i d k

Many scientists say that there is a good reason for gaps in the fossil record. Can you explain why some extinct animals and plants were never fossilized?

Answers

Answer:

For an organism to become a fossil, it must not decompose or be eaten. This can happen if the organism either lives within or is moved to a place where it can be buried and kept from decaying. When an organism is buried quickly, there is less decay and the better the chance for it to be preserved.

Explanation:

I copied and pasted from https://www.americangeosciences.org/education/k5geosource/activities/investigations/fossils/how-fossils-form ,it might help for your work.

O
Facial expressions - How you look in front of your audience based
on your face can affect how they perceive you. If you are nervous
and cannot control it, your fear often shows on your face. If you
smile at them while you talk, they will think that you are happy
talking to them.​

Answers

so what's the question here??

3
In how many revolutions can a bicycle
wheel of diameter 4.9 m cover a dis-
tance of 46.2 km?​

Answers

Answer: :Total distance to be travelled = 4.4 km

= 4400 m

Diameter of wheel = 70 cm = 0.7 m

Radius of wheel= 0.7 / 2 m

Circumference of wheel = 2 * pi *r

= 2* 22*0.7 / 7* 2

= 22*0.1

= 2.2 m

No. Of revolutions = Total distance / Circumference of wheel

= 4400 / 2.2

= 2000

Explanation:

Please help me with this page

Answers

Answer:

2.D  3.nitrogen and oxygen 4.at the poles 5. 8 minutes and 20 seconds 6.B

Explanation:

Does anyone have a flower diagram with all the labels? It would really help

Answers

Answer:

here here it helps

---------------------------

3. Most of the traits expressed in a person's phenotype are determined by Is ​

Answers

I strongly agree with the Person
Above

Most of the traits expressed in a person's phenotype are determined by is Phenotype.

What is Phenotype?

Phenotype refers to an individual’s observable traits, such as height, eye color and blood type. A person’s phenotype is determined by both their genomic makeup (genotype) and environmental factors.

An observable characteristic is simply referred to as a "phenotype." Pheno, which has the same root as the term "phenomenon," simply means "observe."

It can therefore refer to anything from a common attribute, like height or hair color, to the presence or absence of a disease. It is also an observable type of an organism.

Therefore, Most of the traits expressed in a person's phenotype are determined by is Phenotype.

To learn more about Phenotype, refer to the link:

https://brainly.com/question/20730322

#SPJ6

Which is not a physical change in the digestive system?

A. Chewing food

B. Moving food down the esophagus to the stomach

C. Breaking down food with gastric acid in the stomach

D. Moving waste out of the body

Answers

Answer:

C

Explanation:

it is chemical, not physical

what is the process when an embryo embeds itself into the endometrium​

Answers

Answer:

Implantation is a process in which a developing embryo, moving as a blastocyst through a uterus, makes contact with the uterine wall and remains attached to it until birth. The lining of the uterus (endometrium) prepares for the developing blastocyst to attach to it via many internal changes.

Explanation:

☁️ Answer ☁️

Implantation

1. Implantation is the process where the embryo attaches to the uterine lining (endometrium) and embeds itself into the tissue. This happens around Day 5 or so of the embryo’s development.

Link: https://www.remembryo.com/embryo-implantation/#:~:text=Implantation%20is%20the%20process%20where%20the%20embryo%20attaches,Day%205%20or%20so%20of%20the%20embryo’s%20development.

2. Implantation is a process in which a developing embryo, moving as a blastocyst through a uterus, makes contact with the uterine wall and remains attached to it until birth. The lining of the uterus (endometrium) prepares for the developing blastocyst to attach to it via many internal changes. Without these changes implantation will not occur, and the embryo sloughs off during menstruation.

Link: https://embryo.asu.edu/pages/process-implantation-embryos-primates

Hope it helps.

Have a nice day noona/hyung.

Name two types of lateral pathways for the movement of sucrose from source cell into the sieve tube

Answers

Answer:

Diffusion and active transport.

Explanation:

Diffusion and active transport are the two types of lateral pathways that is responsible for the movement of sucrose from source cell into the sieve tube. Sugar molecules are moved into the sieve elements which are the phloem cells through active transport i.e. with the use of energy. The water produces turgor pressure in the sieve elements, which pushes the sugars and fluids down the phloem tubes toward the storage sites. it also moved through simple diffusion due to difference in concentration i.e. from higher to lower concentration region.

Dead leaves and dead trees are? Abiotic or Biotic 

Answers

Answer:

Biotic

Explanation:

Hope this helps and have a wonderful day!!!!

based on the information on the map where are the most stable ecosystems usually found?

Answers

The correct answer is C. Near the equator.

Explanation

The image shows a world map in which there is a horizontal black line in the middle that represents the equator line and some red areas. These areas are the most stable ecosystems in the world since due to their proximity to the equator the incidence of the sun's rays is more direct. Also, in these areas, there is no weather pattern defined by seasons, but climates are defined by altitude, that is, meters above sea level. Therefore, the correct answer is C. Near the equator.

Answer: c

Explanation:

I took the test :)

Do you think a flowchart is the best way to explain how the nervous system works to someone who’s unfamiliar with the concept? Explain your answer.

Answers

Answer:

No, it’s not the best way. The flowchart shows the process step by step, but the intricacies would only be clear to a person who knows the major parts of the nervous system and how they interact with each other.

Edmentum and Plato sample answer

i need help with this biology question pls and thankyou pls help

Answers

The answer to this question is #2 it has guanine and ribose!

Human caused reductions of biodiversity, include pollution, invasive species, and overexploitation.
O True
O False

Answers

Answer:

True

Explanation:

Development, together with globalization, drove severe changes at a global level. The natural and original environments suffered from these effects and still are.

Human beings have constantly been altering the natural ecosystems and causing irreparable damages to nature. For many decades, development involved society and technology advance over new areas unpopulated by humans.

Some of the principal human activities responsible for biodiversity loss are

the uncontrolled use of unrenewable resources, overexploitation of renewable resources; land destruction for the mining industry, farming and cattle industry, buildings, and other infrastructures; the unplanned advance of cities, and opening of new roads to connect those cities.  

All of them involving

deforestation and fragmentation of natural landscapes; water, air, and soil pollution, species loss, and reduction of biodiversity.

With globalization, transport around the world became normal. And when traveling around the world, new species are intentionally or unintentionally transported too. When these species, of animal or vegetable origin, get established in a new area, they overgrow to the point of turning invasive.

Invasive species are uncontrollable and cause severe damage in native endemic species by competing for resources. The whole ecosystem structure changes.

But there are some background glitches in the system that are the principal responsible for all the mentioned activities. Some important aspects are lacking

The respect for nature, People´s education at different levels to care and protect wildlife, Interest in keeping ancestral knowledge, Planning and previous environmental impact studies, Policies to control human activities, Caring involved government.

If, as a society, we lack these aspects, the possibilities of changing our impact on nature are far away.  

What is Nuclear Transplantation?

Answers

Answer: Nuclear transfer is a form of cloning. The steps involve removing the DNA from an oocyte, and injecting the nucleus which contains the DNA to be cloned. In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state.Nuclear transfer is a form of cloning. The steps involve removing the DNA from an oocyte, and injecting the nucleus which contains the DNA to be cloned. In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state.

Explanation:

The s and p shells of an atom are full. Its atomic number is

Answers

Answer:

10

Explanation:

The maximum number of electrons in first orbit or P-shell will be 2×12=2 and second orbit or S-shell will be 2×22=8. Hence, if K and L shell are full, the total number of electrons in the atom will be 2+8 = 10.

Biology 1 Post Lab Questions: 1. Which method appear · which method appeared to have the most dramatic denaturing effect on egg albumin? Why do you think this method had a greater effect?

Answers

Hello. You have not introduced the methods used to denature the egg albumin. However, the most efficient protein denaturation method is the heating method.

The denaturation of a protein, such as albumin, occurs when an element causes the complete destruction of the protein's three-dimensional structure, in a completely irreversible way. The best way to do this is through heat. In this way, through a heating method, the heat breaks the hydrogen bonds that promote the structure of the protein, leaving it completely denatured.

What happens to species diversity WITHOUT Pisaster? *

Answers

Answer:

The starfish Pisaster ochraceus is a keystone species in the rocky marine intertidal communities off the northwest coast of North America. This predatory starfish feeds on the mussel Mytilus californianus and is responsible for maintaining much of the local diversity of species within certain communities. A keystone species is an organism that helps define an entire ecosystem. Without its keystone species, the ecosystem would be dramatically different or cease to exist altogether. This means that if the species were to disappear from the ecosystem, no other species would be able to fill its ecological niche.

Explanation:

Hope this helps!

Which states of matter is water in during the water cycle?

Answers

Answer:

Explanation:

The water cycle is the path that all water follows as it moves around our planet.

Someone help please

Answers

Answer:

ATP hope that helps you out good luck

In order to attract females, the male Bowerbird of Australia constructs elaborate structures called "bowers" on the forest floor. His bower is made from twigs, leaves, and moss, and is decorated with colorful ornaments such as feathers, pebbles, berries, and shells. If a female is sufficiently impressed with a bower, she will mate with the male who built it. This is an example of which of the five evolutionary forces

Answers

Answer:

non-random mating

Explanation:

The five different forces that influence species evolution are 1-non-random mating, 2-mutation, 3-gene flow, 4-genetic drift, and 5-natural selection. Non-random mating refers to phenotypic/behavioral differences that lead to preferential mating among individuals in a population (sexual selection). Mating behavior is a type of non-random mating pattern that includes mate choice (sexual selection) and increases reproductive fitness by increasing the chances of producing descendants ​(offspring).

Are floating cities the solution to rising sea levels???
(HELP ASAPP!!!!!!!!!)
(resources for evidence please)

Answers

Answer: Floating Cities Aren't the Answer to Climate Change

UN-Habitat is looking at high-tech urban islands as a potential survival fix for communities at risk from rising seas.

Explanation:

Floating Cities Won't Save Us From Climate Change ...https://www.bloomberg.com › news › articles › floating-ci...

name and describe the process occurring in the nucleus wich results in the formation of an mRNA molecule.​

Answers

Answer:

The correct answer is - transcription.

Explanation:

Transcription is the process that takes place in the nucleus and the final result of the process is an mRNA molecule that encodes the information of a specific DNA sequence.

Transcription in prokaryotes is carried out in three stages.  

Initiation: Here, RNA polymerase binds to the promoter region and transiently binds to the ‘initiation factor’ to initiate transcription.

Elongation: This is the step where the RNA strand starts getting longer. RNA polymerase “walks” along one strand of DNA. For every nucleotide recognized on the DNA template, it adds a complementary RNA nucleotide to the growing RNA transcript.

Termination: Transcription stops once the RNA polymerase reaches the terminator region. At this region, the RNA transcript and the RNA polymerase, both fall off. RNA polymerase transiently associates with the ‘termination factor’ to stop transcription.

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