Histones are small proteins with a strong positive charge at physiological pH. They are found in the nuclei of eukaryotic cells and package DNA into nucleosomes, the fundamental structural units of chromatin. Based only on this description, what two forms of chromatography could you use, one after the other, to separate histones from other cellular proteins? Explain your reasoning.

Answers

Answer 1

Answer:

Two modes of chromatography that could be used over and over, based on characteristics of histones throughout the issue, are:

Size-exclusion Chromatography Ion exchange chromatography

Explanation:

Using size exclusion chromatography:

A stationary step, consisting of such a polymeric material with numerous pores of various sizes. The larger proteins would travel further as the solvent system comprising the analytes flows through the paper chromatography and therefore will be separated first. That was because their greater size does not in itself allow the pores found in the polymer to get trapped. Tiny proteins such as histones, and on the other hand, can pass slowly across the process to have been eluted later.

Using Ion exchange chromatography:

As even the histone properties at biological pH are strongly positively charged, through the use of highly reactive particles as when the stationary step will allow histones to connect to themselves and now all the proteins, as well as favorable proteins that are poorly absorbed, would be eluted. Many positively charged molecules, that aren't as highly charged at around the same pH as histones, will stay throughout the stationary step, however, based on whose net charge, should flow independently of histones.

Answer 2

The two classes of chromatography techniques include size-exclusion and ion exchange chromatography.

What are size-exclusion and ion-exchange chromatography?

Chromatography is a technique used in molecular biology laboratories to separate biomolecules.

Size exclusion chromatography is a technique capable of separating biomolecules according to size by using filtration in a gel.

Size exclusion chromatography is a technique capable of separating biomolecules and ions according to their affinity to a given ion exchanger.

In conclusion, the two classes of chromatography techniques include size-exclusion and ion exchange chromatography.

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Related Questions

Describe what happens in the ocean with plate tectonics

Answers

Answer:

As tectonic plates slowly move away from each other, heat from the mantle's convection currents makes the crust more plastic and less dense. ... This rock (basalt) becomes a new part of Earth's crust. Mid-Ocean Ridges. Seafloor spreading occurs along mid-ocean ridges—large mountain ranges rising from the ocean floor.

the person above me is correct btw you should give them brainliest now !

define asexual reproduction help plz​

Answers

Reproduction occurring without the sexual union of male and female gametes. :))

IF you see something dark or with a pole like figure then run and hide if you hear what it says it already too late

Answers

Answer:

stop being spoopy im scurred (._.)

Explanation:

eh still not scarry

       ~( ̄▽ ̄)~

(e) Describe the role a non-competitive inhibitor would play in the ability of enolase to perform
properly.

Answers

Answer:

it would not allow enolase to perform properly because it will bind to it and change its shape not allowing 2-phosphoglycerate to bind to it and convert to phosphoenolpyruvate. this is because it is non-competitive which means there is no way that the molecule could bind to the enzyme.

hope this helped!

Help me please if u don't know don't answer please​

Answers

Answer:

I would love to help but I got that answer wrong before and I keep trying over and over again

Explanation:

What does this model imply about the relationship between two processes All but one choice could apply

Answers

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The Channichthyidae fish, also known as the

crocodile ice fish, lives in the Southern Ocean

around Antarctica. These waters are known to

maintain extremely low temperatures all year,

ranging from approximately 28°F to 35°F. These fish

have adaptations that allow them to live in cold, low

oxygen waters. (a) If you were to examine the

membrane lipid composition of cells from the

Channichthyidae fish identify what you would

expect to find. (b) Justify why you might find what

you identified in part (a).

Answers

A) If you were to examine the membrane lipid composition of cells from the Channichthyidae fish, what you would expect to find is glycoprotein,zona pellucida protein and a less number of haemoglobin that is found in this type of fish.

B) Justification of why you might find what was identified in part (A) is because;

Glycoprotein contains antifreeze compounds,these antifreeze compounds are what provide resistance to freezing of their hypoosmotic tissues by the subzero environment.

If we analyzed the lipid composition of these fish, we could find glycoproteins.

We can arrive at this answer because:

Glycoproteins are formed by a chain of oligosaccharides, linked to a chain of polypeptides, which are side chain lipids.These glycoproteins have different functions in the body.One of the most important functions is keeping the heat.This function allows heat to remain longer in an organism and heat loss to be slower.

In this case, the glycoprotein is an essential molecule in the organism of living beings that live in environments with very low temperatures, such as fish Channichthyidae.

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What is inside a seed?

Answers

Answer:

inside a seed is a plant called the embryo

Explanation:

if u need more information msg me but i hope  this helped u

Answer:

inside the seed will be a tiny plant cell called the embryo

Calculate the force needed to cause a 6 kg bowling ball to accelerate 20 m/s2.

Answers

Answer:

Explanation:

F = m * a

m = 6 kg

a = 20 m/s^2

F = 6 * 20

F = 120 N   which is short for Newtons.

Which equations are used to calculate the velocity of a wave?

Answers

Answer:

The Wave Equation

Multiplying the wave's frequency by its wavelength, which is its physical length, yields the velocity of the wave.

Explanation:

Answer:

Just v = f × λ

v is the wavespeed in m/s, f is the frequency (Hz) and λ is the wavelength (m)

The energy given off by burning fossil fuels can be measured in
A) Joules
B) calories
C) Hertz
D) electronvolts​

Answers

Answer:

A

Explanation:

A

Answer:

A

Explanation:

Joules are used to measure energy

What are functions of the roots in seed plants? (Choose all that apply)
stores food for the plant
aids in reproduction of the plant
anchors the plant
absorbs nutrients for the plant

Answers

Answer:

stores food for the plant

aids in reproduction of the plant

absorbs nutrients for the plant

China rose is so beautiful.explain the colour and why rose is important for our life.

Answers

Answer:

Explanation:

China rose converts acidic solution to dark pink, basic solutions to green colour and has no colour change for neutral solutions. .  The leaves, fruits and roots are decocted and used in the treatment of arthritis, boils, coughs etc. The fruit is applied to sprains, ulcers and wounds. The flower buds are used in the treatment of dysmenorrhoea, poor circulation, stomach pains and swellings.

What is crossing-over, during what phase does it occur, and what is its significance?

Answers

Answer:

Crossing over is a biological occurrence that happens during meiosis when the paired homologs, or chromosomes of the same type, are lined up. Hope this helps :)

Explanation:

Please answer
Answer answer answer answer answer answer answer

Answers

Answer:

The nucleus is like the principal of a school, the cell membrane is like a school building, and the mitochondria is like the staff in a school.

Explanation:

Definitions:

The nucleus of a cell contains the majority of the cell's genetic material, and coordinates cell activities like protein synthesis and cell division.The cell membrane is the semipermeable membrane of a cell that surrounds the cytoplasm (the material inside the cell other than the nucleus).The mitochondria is "the powerhouse of the cell", because it produces most of the chemical energy needed to power the cell's chemical reactions.

The nucleus is like the principal of a school: the nucleus controls the cell, much like how the principal controls the school.

The cell membrane is like a school building: the cell membrane forms a boundary between the cell and outside environment, much like how the school building separates you from outside.

The mitochondria is like the staff in a school: the mitochondria powers the cell, much like how the staff in a school keep the school running.

Answer:

Answer

Explanation:

Answer

Digestion and breathing are _____. controlled by ganglia and plexus reflexive actions controlled by the spinal cord voluntary actions controlled by the central nervous sytem

Answers

Answer:

The correct answer is - reflexive actions controlled by the spinal cord.

Explanation:

Digestion and breathing are examples of involuntary action which is controlled by the autonomic nervous system of the CNS. however, the part of the nervous system that controls is the spinal cord and hind brain by the spinal reflex actions.

These are the action they do not require any thought and work without any thought that leaves the brain for other actions and coordinations. specific but the same stimuli causes the response in the same way.

this is the pic

it's science btw​

Answers

5? ig thats my guess not sure

T/F Osmosis and diffusion are both examples of passive transport.
A.True
B.False

Answers

Both osmosis and diffusion are passive transport processes, meaning that no additional energy is needed for them to take place. In both diffusion and osmosis, particles move from an area of higher concentration to one of lower concentration.

The given statement "Osmosis and diffusion are both examples of passive transport" is True.

Passive membrane transfer methods are the most direct. Passive transport is a phenomena that happens spontaneously and doesn't require the cell to use energy to move. Diffusion is the process by which chemicals travel passively from a region of higher concentration to a region of lower concentration. A concentration gradient is a difference in the concentration of one substance throughout a physical region.Transport that is done passively is called diffusion. Until the concentration is the same across the space, a single substance has a tendency to travel from an area of high concentration to an area of low concentration.Based on the gradient of water concentration across the membrane, osmosis is the mechanism by which water diffuses through a semipermeable membrane. Osmosis moves just water across a membrane, and the barrier restricts the diffusion of solutes in the water, whereas diffusion distributes material across membranes and into cells.

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5. Some advantages of sexual reproduction include: (select all that apply) *

a.genetic variation
b.improves chances of adapting to the envionment
c.helps species survive catastrophes
d.Certian genes work beter than other

Answers

The answer is A, genetic variation

Some advantages of the sexual reproduction include genetic variation. Genetic variation results into changes in the genetic material (DNA). Thus, the correct option is A.

What is Genetic variation?

Genetic variation is the difference in the DNA (deoxyribonucleic acid) among individuals or the difference between different populations. The multiple sources of genetic variation include mutations and genetic recombination. Mutation is the changes in the genetic material of an organism which are the ultimate sources of genetic variation, however other mechanisms, such as genetic drift, contribute to it, as well.

Genetic diversity is important because it gives the species a better chance of survival through variations. However, the genetic diversity can be lost from the species when the populations get smaller and isolated from each other, which decreases a species' ability to adapt and survive better in the condition.

Therefore, the correct option is A.

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Which of the following IS found in a bacteria cell?

Answers

A procaryotic cell has five essential structural components: a nucleoid (DNA), ribosomes, cell membrane, cell wall, and some sort of surface layer, which may or may not be an inherent part of the wall.

When you and your friends work together to find a solution to a project you are:
o mediating
o competing
o cooperating
o assisting

Answers

Answer:

Cooperating

Explanation:

Cooperation is "the process of working together" so if you and your friends are working together, you are cooperating. Hope this helps! :)

Answer:

c

Explanation:

any three importance of measurements ​

Answers

descriptive, diagnostic, and predictive. Descriptive is the most basic form of measurement.

the egg of an animal is most like which part of a plant

Answers

Answer:

It is the seed of a plant because both have to break open to start a life. Hope this helps please reward brainlist.

Using the above diagram, what does the body make in response to a vaccine?

Answers

Answer:

antibodies

Explanation:

Vaccines are like a training course for the immune system. They prepare the body to fight disease without exposing it to disease symptoms. When foreign invaders such as bacteria or viruses enter the body, immune cells called lymphocytes respond by producing antibodies, which are protein molecules

i dont know the answer to this please help

Answers

Answer:

b is the correct answer

whatever the answer is look it up

Vhat is the correct formula for trisilicon hexafluoride.

Answers

Answer:

xenon hexafluoride.

Explanation:

Answer:

Si₃F₆

Explanation:

Which of the following would a lipid most likely do in the body?

А. provide quick energy for exercise

B. store genetic information

С. build muscle tissue

D. store energy for later use

Answers

Answer:

D

Explanation:

Lipids have a carboxyl group at one end, a methane group at the other, and a long hydrocarbon chain in between. This structure makes it suitable for long-term energy storage. Quick energy is usually provided by carbohydrates. Nucleic acids store genetic information, and muscle tissue is made up of muscle cells.

The most likely function of a lipid in the body is the storage of energy for later use. Choice D.

Although, lipids are a good source of energy with about 9kcal of energy supplied per gram.

Lipid metabolism to provide quick energy is quite a slow process as opposed to carbohydrates which provide quick energy for exercise and other tasks.

Nucleic acids are responsible for storing genetic information.

Muscle tissues are built by muscle cells.

As lipids are a high energy yielding source, lipids are responsible for storing energy for later use.

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PLS HELP ME!!!!!!!!!!!!!!!!!!!!!!

Answers

Answer:

water/erosion- when water is in rocks/ minerals it erodes them and causes them to fall apart. water/deposition-water brings other materials into the land and they're sometimes formed into the rock i could even be previous weathered materiel. wind/erosion- wind can cause things to fall off of big boulders/mountains/rocks and cause them to fall apart. wind/deposition- wind can carry other materials onto the eroded land.

Explanation:

i passed grade 6 lol.

Which is an example of radiation?

A melting snowman sweats drop of water under a bright sun.
A smiling man roasts marshmallows over a wood fire.
A cook stirs vegetables in a frying pan over a gas burner.
Three pairs of bare feet and lower legs walk across black pavement.

Answers

Answer:

A: melting snowman sweats drop of water under a bright sun

Answer:

The correct answer is A.

From how many stem cells does cell division take place in meiosis?

Answers

Answer:

They probably divide to make EITHER two stem cells, OR two more specialized cells.

Explanation:

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