The primers you used in lab amplified 145 base pairs that were adjacent, but not included in the repeat. If you used a different primer that only amplified 130 base pairs adjacent to a 14 base pair repeat, and your PCR product size was 172, how many repeats were in your product?
6
5
1
4
3
2

Answers

Answer 1

Answer:

3 repeats

Explanation:

PCR technique used to make multiple DNA copies from a segment or small sequence of DNA that is called primer for identification or detection of the pathogen.

given:

Primer amplified base pairs - 130  that are adjacent to - 14 base pair repeats.

PCR product size was 172

So, the extra nucleotide that we get would be = product size - primer amplified

172- 130 = 42 bp

Extra repeats you got = 42/14= 3 repeats


Related Questions

Identify and describe two systems that interact with the digestive system to deliver nutrients to
cells.

Answers

Answer:

The two systems are circulatory and endocrine.

Explanation:

circulatory absorbs nutrients and it signals through the endocrine to control the speed of digestion.

What is your diagnosis choose from these two options cerebral edema or epilepsy

Answers

Answer:

cerebral edema

Explanation:

30. Which of the following is out of sequence?
Earth
solar system
Milky Way
universe
moon

Answers

The solar system is defined as the system of the Sun, planets, and other celestial objects bound together by gravitational force. The solar system is present in a milky way, which lies in the universe.

The correct sequence of the celestial objects with respect to their size aligned in a Solar system is the moon, Earth, Solar system, Milky Way, and the universe.

The statements can be explained as:

The moon is the natural satellite of the Earth, which revolves and rotates around the Earth in a fixed path.

The Earth is a planet that revolves around Sun and rotates on its own axis.

The solar system comprises the sun, planets, and other celestial objects. The solar system is present in a galaxy.

The milky way is one of the billions galaxy present in the universe. A galaxy has billions of solar systems, which are light-years apart.

Thus, the celestial objects, galaxies, and constellations make up the universe.

To know more about the solar system, refer to the following link:

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Which is the problem when using turbines to produce energy??

Answers

Answer:

C

Explanation:

only applicable answer

Which is the major organ of the immune system?
O kidney
O blood vessels
Olymph nodes
O stomach

Answers

The major organ is the Kidney

Answer:

lymph nodes is the answer

which is the least complex thing in our body

Answers

Answer:

cell

Explanation:

the smallest, least complex structure in an organism

the order from most complex to least complex - organism, organ system, organ, tissue, cell

This would be a cell

Hope it helps :)

why doesn't the person at the dig site fall off Earth?

Answers

May you explain ur question more clearly

Using the data from the paraquat experiment, explain how you know that paraquat did not cause the coral bleaching.​​​​​​​

Answers

Answer:

paraquat is a herbicide.

Explanation:

Paraquat did not cause the coral bleaching because paraquat is a herbicide which is used to kill weeds not algae. We know that coral have small algae attached to its body which is responsible for its colour so if we apply paraquat, it does not kill the algae and so coral bleaching did not occur. if we apply another chemical which kills algae so it causes coral bleaching and the coral becomes white.

Beginning with solar energy, detail how transfer of energy drives weather and climate. Be sure to mention how energy transfer affects wind, rain, temperature, and climate.

Answers

Answer:

So your answer for this question is weather and climate involves with radiation as the transfer of energy. And the rest is conduction and radiation.

Explanation:

Well solar energy is from the sun and the transfer of energies for heat is convection, conduction and radiation. Now electrically, mechanically is ways actually energy can be transmitted. But for weather and climate energy can be transferred there by radiation energy. And "wind, rain, temperature , and climate" are affected by conduction and radiation.

The solar energy affects climate, wind, rain, temperature as follows:

Solar energy is a renewable form of energy, obtained from the transformation of electromagnetic radiation that comes from the Sun.

The Earth's climate is primarily a manifestation of how solar radiation is absorbed, redistributed in the atmosphere-ocean system, and subsequently re-radiated into outer space.

Climate and weather depends on how this energy is distributed between the atmosphere and the earth's surface.

The amount of energy absorbed by the surface depends on latitude, causing pressure changes in the atmosphere and forming wind currents.

The abundance of water vapor in the atmosphere leads it to condense in the form of clouds, which, when displaced by the wind, collide with each other and release their liquid content, in what we know as rain.

In terms of temperature, the climate is warmer where more energy reaches the surface, and colder where less.

Also, atmospheric temperature is about the degree of heat or cold that exists in atmospheric air masses, heated mainly by solar radiation.

Therefore, we can conclude that solar energy is the energy source for all life on Earth, where most of the solar energy reaches the surface in the form of light and heat and the climate depends on how this energy is distributed between the atmosphere and the earth's surface.

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Considering aesthetics means thinking about a product's

Answers

Answer:

Aesthetics is about . You may think of aesthetics as distinctly different from a product’s function, but it’s not. To be considered great design and desirable to users, a product must both function well and exhibit good design with attributes like beauty, smart ergonomics, and a form that aids in function.

Answer:

Functionality

Explanation:

A____ is the smallest particle of an element that has the chemical properties of that element

Answers

Answer:

the answer is an atom :)

The Energy Policy Act of 2005 was for the most part a hard-path or business-as-usual proposal.

Which of the following is a characteristic of this act?

A)

It promotes the exploration and use of fossil fuels.

B)

It emphasizes energy quality.

C)

It relies mostly on renewable sources of energy.

D)

It promotes an increase in second-law efficiency.

E)

It has no effect on the environment.

Answers

Answer:

b is correct answer in my view I think if it's wrong then Correct me

It promotes the exploration and use of fossil fuels. Therefore, option (A) is correct.

What is The Energy Policy Act of 2005?

The Energy Policy Act of 2005 was a controversial legislation that was passed by the US Congress in 2005. It had several provisions that were intended to promote the exploration and use of fossil fuels, such as oil, natural gas, and coal.

These provisions included tax incentives for the production and consumption of fossil fuels, as well as relaxed regulations on the exploration and extraction of these resources. The act was largely seen as a "business-as-usual" proposal, as it did not significantly shift the focus of US energy policy away from fossil fuels and towards renewable energy sources.

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When a protein needs to be made, a signal is sent to a cell to turn on the ___B___ that codes for the needed protein. This is the start of transcription. Transcription occurs inside the ___C___ (cell organelle). During transcription, DNA is read by an enzyme called ___D___ and produces ___E___, abbreviated mRNA. This newly created mRNA molecule moves to the ___F___ (part of cell) where the process of translation can occur. During translation, small organelles called ___G___ read the mRNA sequence. These organelles direct transfer RNA, or tRNA, to assemble a specific sequence of ___H___ (2 words). A ___I___ is a sequence of three mRNA bases that codes for one amino acid. When all the amino acids coded for by a gene link, they fold, and this creates a functional ___J___.

Answers

Answer:

B: gene

C: nucleus

D: RNA polymerase

E: messenger RNA

F: cytoplasm

G: ribosomes

H: amino acids

I: codon

Explanation:

When you look at the protein synthesis process and the steps, this is how it all occurs. (: Hope this helped.

Filling up the gaps in the your question with the correct answers ;

A signal is sent to a cell to turn on the gene ( B ) Transcription occurs inside the Nucleus ( C )DNA is read by an enzymes called RNA polymerase ( D ) and produces messenger RNA ( E )Newly created mRNA molecule moves to the Cytoplasm ( F ) During translation small organelles called Ribosomes ( G ) read mRNA A specific sequence of Amino acids ( H ). A codon ( I ) is a sequence of three mRNA bases that codes for one amino acidThis creates a Functional Protein ( J )

Transcription is simply the synthesis of RNA from DNA by the conversion of a DNA code to a complementary RNA code while

Translation is the synthesis of protein from mRNA by the conversion of mRNA code into an amino acid sequence which is contained in the protein

Hence we can conclude that the answers to your question are as written in the gaps left in your question above.

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Ji wants to write an example of a frameshift mutation using the following sentence: THE CAT SAW THE RAT. Which sentence best shows a frameshift mutation?

Answers

Answer:

This question lacks options, the options are:

A) THC ATS AWT HER AT

B) CAT THE SAW RAT THE

C) EHT TAC WAS EHT TAR

D) THE THE CAT SAW THE RAT

The answer is A

Explanation:

Mutations are generally any change that occurs to the nucleotide sequence of a gene. However, a FRAMESHIFT Mutation is a kind of mutation that changes/alters the reading frame of the nucleotide sequence. Frameshift mutations are caused when either a DELETION (removal of nucleotides) OR INSERTION (addition of nucleotides) MUTATION occurs.

In this question, Ji wants to write an example of a frameshift mutation using the following sentence: THE CAT SAW THE RAT. A frameshift mutation will delete or add nucleotide bases to the sequence. Hence, the sequence: THC ATS AWT HER AT best represents frameshift mutation because a deletion mutation has altered the reading of the sequence in three's.

Notice that the bases in options B, C and D are still read in three's.

Answer:

THC ATS AWT HER AT.

Explanation:

can I please get 2 paragraph about water cycle.Note if you put something dumb i will report thanks ​

Answers

Answer:

Here is my water cycle report

Water is evaporated, then is condensed and then it makes the clouds form, and precipitates back to earth, in the form of rain, or snow.

Explanation:     All of these stages are caused by the sun, and the sun helps them rotate, and cause a cycle

the mass of a material is

Answers

Answer:

The density (more precisely, the volumetric mass density; also known as specific mass), of a substance is its mass per unit volume. The symbol most often used for density is ρ (the lower case Greek letter rho), although the Latin letter D can also be used.

Explanation:

Answer:

370.3 x g

Explanation:

Which of the following kingdoms contains only multicellular autotrophs?
A Protista
B. Animalia
C. Fungi
D. Plantae

Answers

Answer:

d

Explanation:

because it is

The answer is d
Hope this help!

Think about scary movies you have seen. Music often helps to make scary scenes even more
frightening. What elements of music can make it sound scary? What music sounds most
frightening to you?
Help please

Answers

Answer:

Scary music may be scary because of the soundtrack. Maybe Halloween music can be frightening because it is mostly quiet and spooky. An element to make music frightening is probably quietness. Sometimes, if it sounds like a quiet rollercoaster, it is scary music.

Explanation:

please help I need it in five mins​

Answers

Answer:

a or 1

Explanation:

which of the following is always true of animals that are products of sexual reproduction
[[[35 POINTS!!!]]]
A. They hatch from eggs.
B. They are nursed y their mothers.
C. They are identical to one of their parents.
D. They have genetic material from two parents

Answers

Answer:

Which of the following is always true of animals that are products of sexual reproduction?

Explanation:

They have genetic material from two parents.

.

They have genetic material from two parents is true about sexual reproduction.

What is sexual reproduction?

Sexual reproduction is the process of combining the genetic information of two individuals of the opposite sex to create new organisms. Genetic information is carried on chromosomes within the nuclei of gametes, which are specialized sex cells.

These gametes are called sperm in males and eggs in females. During sexual reproduction, two gametes participate in a fusion process called fertilization to create a fertilized egg.

The fertilized egg is the progenitor cell of the embryonic offspring and receives half of its DNA from each parent. In humans, the fertilized egg contains 46 chromosomes.

Therefore, They have genetic material from two parents is true about sexual reproduction.

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1.) Which of the following is not true of negatively supercoiled DNA?

A. Eases the separation of nucleotide strands during replication and transcription.

B. Allows DNA to be packed into small spaces.

C. Has less than 10 bp per turn of its helix.

D. Is more negatively charged due to additional phosphates per turn of the helix.

E. Is found in most cells.

2.)How many complete rotations would most likely correspond to a positively supercoiled DNA molecule that is 100 bp in length?

A.) 0

B.) 5

C.) 10

D.) 15

E.) 100

Answers

Answer:

1.

C. Has less than 10 bp per turn of its helix.

2.

D.) 15

Explanation:

Negatively supercoiled DNA

(deoxyribonucliec acid) is the twisting against the helical conformation which is a twist in a left handed direction that loose and straightens the helix at low twisting stress, and knot the DNA into negative supercoils at high twisting stress.

Supercoiling will occur where the DNA helix must uncoiled for the function of a macromolecular assembly.

Supercoiling allows DNA to be packed into small spaces, It allows for more negative charge and can be found in many cells. But it does not have less than 10 bp per turn of its helix.

15 rotations will correspond positively supercoiled dna molecule of 100base pair.

Complete the following statements:
• The LONGER the instrument, the_________
the PITCH.
•The SHORTER the__________instrument, the
the PITCH.

Answers

Answer:

longer is lower shortwe is higher

Question 10
Which body systems are involved in the reflex action shown in the diagram?
ОА
the respiratory and excretory systems
B
the excretory and muscular systems
CO) O
с
the muscular and nervous systems
D
the nervous and digestive systems

Answers

Answer:
C. the muscular and nervous systems

What is the Kyoto Protocol?
A) a new technology for cars
B) a set of laws made in Japan that deals with how cars are made
C) a way of going about doing science on the atmosphere
D) a document that sets out a plan for reducing carbon gases

Answers

Answer:

D

Explanation:

Answer:

D) a document that sets out a plan for reducing carbon gases

Explanation:

50 POINTS!!!! Which result is a predicted result of the melting of the world's ice sheets?
A.) Sea levels around the world may rise.
B.)Greenland will become a subtropical zone.
C.)More drinking water will become available.
D.) Native Inuit peoples will become extinct.

Answers

The answer is A I took the k12 test

A predicted result of the melting of the world's ice sheets is option "A" that is Sea levels around the world may rise.

How does melting ice sheets affect climate change?

When warming temperatures slowly liquefy sea ice over the period, more periodic bright covers are open to reflect sunlight back into the atmosphere.

More solar energy is soaked at the surface and ocean temperatures rise. This starts a process of warming and melting.

Thus,  option "A" that is Sea levels around the world may rise.

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an amino acid is deaminated. this is the removal of

Answers

Answer:

an amino acid group from a molecule

Answer:

Deamination is the removal of an amino group from a molecule.

A
B
Identify the organelles labeled on the cell to the
right.
A
B
С
D
D
E
E
F
F
DONE

Answers

The given image shows the structure of the animal cell. The labeling A represents the nucleus, B is the lysosome, C is the Golgi body, D is the cytoplasm, E is the cell membrane, and F is mitochondria.

What is an animal cell?

Animal cells are eukaryotic cells that are surrounded by a plasma membrane and contain a membrane-bound nucleus and organelles.

Animal cells, unlike eukaryotic cells from plants and fungi, lack a cell wall.

The given image shows the structure of the animal cell. The labeling A represents the nucleus, B is the lysosome, C is the Golgi body, D is the cytoplasm, E is the cell membrane, and F is mitochondria.

Thus, labeling  A represents the nucleus, B is the lysosome, C is the Golgi body, D is the cytoplasm, E is the cell membrane, and F is mitochondria.

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5. The test report above shows the level of molecules in different parts of the patient’s body. How would you expect this patient to do on an exercise test?

Answers

Answer:

Poorly; his digestive system is not breaking down molecules from food into glucose.

Explanation:

What is one product of cellular respiration?

Answers

Answer:

ATP

Explanation:

cellular respiration makes a lot of ATP; i think its like 38

What do bears prefer to eat most

Answers

Answer:

only the basic bears

Explanation:

Black bears are very opportunistic eaters. Most of their diet consists of grasses, roots, berries, and insects. They will also eat fish and mammals—including carrion—and easily develop a taste for human foods and garbage.

Alaskan brown bears are opportunistic eaters and will eat almost anything. Their diet consists of berries, flowers, grasses, herbs, and roots. They get their protein from beavers, deer, caribou, salmon, carcasses, and other small mammals.

Polar bears feed mainly on ringed and bearded seals. ... When other food is unavailable, polar bears will eat just about any animal they can get, including reindeer, small rodents, seabirds, waterfowl, fish, eggs, vegetation (including kelp), berries, and human garbage.

A panda's daily diet consists almost entirely of the leaves, stems and shoots of various bamboo species. Bamboo contains very little nutritional value so pandas must eat 12-38kg every day to meet their energy needs.

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