a home made protocol for animal tissue DNA extraction?

Answers

Answer 1

DNA (deoxyribonucleic acid) is the hereditary material in almost all living organisms. Most DNA is located in the nucleus.

For extraction of DNA we need  500 ml water,91% Isopropyl Alcohol (rubbing alcohol),Dishwashing liquid, Salt, Food coloring (dark colors recommended),3 clear plastic cups

Steps include  Pour 500ml water in a plastic cup

2. Add one tablespoon of salt to the water

3.dissolved the salt completely

Transfer 2 tablespoons of salt water in a clear plastic cup

2. Vigorously trill the salt water

3. Spit salt water back into the same cup

When you trill the salt water, cheek cells will come out. Add 1ml of clear dishwashing soap to the salt .It will break open the cheek cells and their nuclei walls and this causes the DNA to be released into the salt water.

1. Pour 100 ml isopropyl alcohol plastic cup.

2. Add 3 drops of food coloring, and isopropyl alcohol  in a separate clear plastic cup.

2. Add 3 drops of food coloring, mix them well

DNA is insoluble in isopropyl alcohol so it will promotes the clustering of the DNA, making them visible.

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Related Questions

why it is difficult to accurately quantify the quantum of carbon released from terrestrial ecosystems that are as a result of land-use change.

Answers

It is difficult to accurately quantify the quantum of carbon released from terrestrial ecosystems because Land uses and land cover changes have remarkable environmental outcome at local, regional, and global scales.

In terrestrial ecosystems carbon is stored within biomass, such as tropical and temperate forests. Carbon is transferred to the soil via leaf litter, roots and plant debris upon decomposition. Bacteria contributes in decomposition of dead matter to releases Carbon back to the atmosphere. So the land usage changes may cause intense inference at the regional and global scales for global loss of biodiversity, distresses in hydrogen and carbon cycle .

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Are lipids hydrophilic or hydrophobic? Explain why.

Answers

hydrophobic, lipids have many non polar covalent bonds. where as water has polar covalent bonds. water can only mix with other substances that have polar covalent bonds. so lipid and water don’t mix. just like if you were to try to mix water and oil.

What is osmosis? Describe this process including the types of molecules that move and the energy required. Include in your description details about the areas of concentration on either side of a membrane when osmosis occurs.

Answers

Answer:

Osmosis: Water diffuses through a semipermeable membrane during osmosis, a particular type of diffusion.

Explanation:

Water easily travels across a membrane in a potential gradient from high to low. Osmosis, one of the most important natural processes, is a natural occurrence. A weaker salt solution will often migrate to a stronger salt solution throughout this process. Reverse osmosis is capable of eliminating up to 99%+ of the dissolved salts (ions), particles, colloids, organics, bacteria, and pyrogens from the feed water, yet it shouldn't be relied upon to entirely remove bacteria and viruses. A RO membrane rejects contaminants based on their size and charge. A correctly operating RO system will likely reject any pollutant with a molecular weight more than 200 (for contrast, a water molecule has a molecular weight of 2).

A few calculations are employed for both design considerations and performance evaluation of a RO system. Instrumentation in a RO system shows quality, flow, pressure, and occasionally other information like temperature or operating hours. The following operational metrics are necessary in order to appropriately assess the performance of a RO system, at the very least:

Feeding forcePermeate forceIncrease your pressure.Charge conductivityTransparent conductivitySupply flowfluid permeationTemperature

REJECTION OF SALT %

You may determine from this equation how well the RO membranes remove impurities. It provides information on the system as a whole rather than the performance of each individual membrane. 95% to 99% of most feed water impurities will be rejected by a RO system that is well-designed and has RO membranes that are operating effectively (that are of a certain size and charge). The following equation can be used to calculate how well the RO membranes are eliminating contaminants:

Conductivity of Feed Water - Conductivity of Permeate Water 100; Salt Rejection%

Feed Conductivity

The system works better the higher the salt rejection. A poor rejection of salt may indicate that the membranes need to be cleaned or replaced.

PASSAGE SALT

 This is the percentage of salts flowing through the RO system in terms of quantity. The method works better the lower the salt channel is. A high salt passage could indicate that the membranes need to be cleaned or replaced.

(1 minus Salt Rejection%) = Salt Passage

RECOVERY

Water that is being "recovered" as good permeate water is measured by percent recovery. The quantity of water that is not drained as concentrate but is instead collected as permeate or product water is another way to conceive of percent recovery. The higher the recovery percentage, the more permeate water is being saved and less water is being sent to the drain as concentrate.

    There are many industrial applications that call for demineralized or deionized water, and reverse osmosis is a reliable and efficient technology to generate water that is suited for these uses. The quality of the RO permeate can be improved through additional post-treatment after the RO system, such as mixed bed deionization, making it appropriate for the most demanding applications. An RO system's proper pretreatment and monitoring are essential for avoiding expensive repairs and unforeseen maintenance. Your RO system should deliver high purity water for many years if it is designed properly, has a maintenance schedule, and is supported by knowledgeable service personnel.

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What would happen if the heart stopped pumping?


A) Oxygen could not be used for cellular respiration


B) Waste could not be removed from the body


C) The lungs would stop taking in oxygen


D) Blood would stop its flow throughout your body

Answers

Answer:

D) Blood would stop its flow throughout your

body

Explanation:

When a Heart stopped pumping then all the blood that flows though out your body will begin to slow down the flow of blood. Although at possible rate Lungs would stop taking in oxygen but the best answer to know first cause is Answer D.

D. Blood would stop its flow throughout your body

Which is NOT one of the factors used
to determine growth rate?
A. number of individuals past the reproducing age
B. number of deaths
C. number of individuals moving into and out of the
population
D. number of births

Answers

Answer:

C

Explanation:

A- Means less births due to less fertility.

B-The deaths means less lives and decrease in growth rate.

C-Does not affect frowth rate.

D-Is increasing growth rate

Which is an example of how a
food resource could be
partitioned?
A. a predatory species feeding on a prey type and
fighting off other species for that source
B. multiple species sharing space at a watering
hole
C. an organism fighting another for a food
source

Answers

Answer:

B

Explanation:

Many species will gain by choosing this option as they all get the water.

Answer: B. multiple species sharing space at a watering

hole

Explanation:

When the product of a reaction becomes the inhibitor of the enzyme that is
catalyzing the reaction, what is occurring?
allosteric activation
allosteric inhibition
feedback inhibition
competitive inhibition

Answers

c) feedback inhibition

Our modern environment may be affecting the endocrine system and reproductive systems in today's young people. Products such as plastics and industrial chemicals are considered endocrine disruptors. Air pollution, pesticides, and other chemicals have a damaging effect on these body systems. How do you think you can protect yourself from being affected from such exposures? What steps should be taken to ensure that people are not overexposed to any harmful substances?

Answers

Some ways to protect from the overexposure to the different kind of contaminants that surround us in this modern environment are:

• Try to consume the most organic and home-made food, avoiding ultra-processed and fast food.

,

• Choose hygiene and cleaning products with the less amount of chemicals possible and use them in adequate amounts.

,

• Use responsibly medicaments, especially antibiotics.

,

• Avoid contaminated areas such us water, or regions very close to farms that are fumigated.

,

• Use sunscreen and avoid solar exposure at the high risk hours.

Steps that should be taken to ensure that people are not overexposed to any harmful substances is to regulate by laws the allowed amouns of this substances in the products we consume, and near the places we live. An example of this is the maximum number of x-rays allowed per person a year due to radiation.

Which condition best represents homeostasis?

having a heart attack
bleeding to death
sweating on a hot day
fracturing a limb

Answers

Sweating on a hot day
Sweating on a hot day since that’s a body’s natural reaction ( stimuli) to a situation .

could someone explain this to me​

Answers

The average beak depth of the surviving birds is between 8.80 to 9.85 mm and the beak depth of non-surviving birds is between 7.52 to 11.20mm. so I would expect that those birds continued to reproduce and pass on the genes for a deeper beak. In subsequent years the beak depth decreased slightly. Generally, the additional variables did not show strong evidence of impacting survival, so beak depth is the trait driving the evolutionary trend. You may wish to add statistical calculations to this question.

What is bird’s beak?

An external anatomical feature called the beak, bill, or rostrum is usually present in birds but can also be seen in turtles, non-avian dinosaurs, a few mammals, and other animals. To eat, groom, manipulate things, kill prey, battle, probe for food, court, and feed young, an animal uses its beak.

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Predict the outcome when two elements are chemically combined? (HS-LSI-1) DOK 2

Answers

The outcome when two elements are chemically combined is a compound and is based on different types of bonds.

What is a Compound?

This is referred to as a substance which is formed as a result of the combination of two or more elements  through the various types of bonding. There are various types of bonding processes and they include electrovalent, covalent etc which have their unique properties and technique.

An element needs to achieve a stable octet configuration which is why they donate or accept electrons. An example of a compound is Calcium chloride which is formed from the reaction between the elements Calcium and chlorine.

The Calcium donates one electron each to two atoms of chlorine which accepts it and forms the compound named above thereby making it the correct choice.

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My question is in the image!!

Answers

C. ADP + P ---> ATP

After completing an internship with UC San Francisco in the Yosemite National Park for 3 months what change would most likely be seen in your blood?

Answers

The change most likely be seen in your blood a website about camping in Yosemite National Park sponsored by a company that sells tour guide services an article about geographical features of Yosemite National Park that includes an author’s name and works cited.

What is website?

Company's name and fame id totally depends upon advertisement and its products and websites play an important role in order to make company popular and due to this reason company used the website or designed the website in unique and knowledgeable manner.

Therefore, The change most likely be seen in your blood a website about camping in Yosemite National Park sponsored by a company that sells tour guide services an article about geographical features of Yosemite National Park that includes an author’s name and works cited.

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Under what circumstances might brighter feathers be an adaptive trait for a male songbird? What about a non-adaptive trait?

Answers

Beaks (bills), feet, and plumage, in particular, are three physical traits that show distinct environmental adaptations (feathers).  Eye color, earlobe size, and the capacity to curl one's tongue are examples of non adaptive traits in humans.

What are some adaptive features of a bird?

Beaks (bills), feet, and plumage, in particular, are three physical traits that show distinct environmental adaptations (feathers). The mode of evolution that produces living things that are adapted to their environments is natural selection.

Similar to how hair regulates body temperature for mammals, a bird's feathers are crucial in controlling body temperature. Camouflage. Some birds have the innate ability to blend into their surroundings, just like many other animals do. This enables them to evade predators or approach potential prey covertly.

A trait that is neither helpful nor detrimental for reproductive success from the perspective of natural selection. Eye color, earlobe size, and the capacity to curl one's tongue are examples of non adaptive traits in humans.

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The muscle is an example of an tissue.Give two reason why the muscle is called tissue ( 2 marks)

Answers

Answer: 1. It is made up of a group of cells.

2. These cells have a well-defined shape, and size and perform a specific function.

Explanation:

A cell is the unit of life. All living organisms are made of billions of cells. These cells have specific functions to play in the sustenance of a living organism.

A tissue is a group of cells that have a definite shape, size, and particular function to play.

The muscle is a group of muscle cells. These are soft tissues. They have an elongated shape and can easily contract and relax.

The muscle tissues give the body a definite structure and help in body movements.

There are three types of muscle tissues. These are soft tissues, striated muscle tissues, and cardiac muscle tissues.

Attaching the appropriate amino acid to a tRNA (i.e. tRNA charging) involves interactions between:

Answers

Attaching the appropriate amino acid to a tRNA (i.e. tRNA charging) involves interactions between activated tRNA and mRNA. This happen during the translation.

mRNA- Single-stranded RNAs of the kind known as messenger RNA, or mRNA, are used to make proteins. mRNA is created during transcription from a DNA template.

Activated tRNA- The process of attaching an amino acid to its corresponding transfer RNA is known as amino acid activation, commonly known as aminoacylation and or tRNA charging (tRNA).

Amino Acid- Small chemicals known as amino acids serve as the foundation for proteins. Proteins carry out several crucial chemical processes as well as providing structural support for the cell. Twenty distinct smaller, simpler amino acid combinations make up the molecules that make up each protein.

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What can strong CMEs affect on the earth
A - CMEs can cause fires on the surface of the earth
B - CMEs can cause damage to power grids by overloading them
C - CMEs can change cloud formation and decrease albedo
D - CMEs can cause radio blackouts due to energetic photons

Answers

Answer:

CMEs can also cause surges in electrical currents which overload power grids causing widespread blackouts. Also, according to NASA, CMEs can jostle Earth's magnetic field which can impair radio transmissions and increase radio static in Earth's ionosphere.

Explanation:

Why are Somali villages with sustainable grazing considered outliers? Based on your answer, how would you define an outlier?

Answers

Despite years of drought, a few Somali settlements have maintained excellent rangeland, making them outliers.

Outliers are data points, people, or communities that deviate from the norm.

This is further explained below.

Somali villages with sustainable grazing:

Generally, Because they have been able to keep healthy rangeland throughout years of severe drought, the few Somali villages that have sustainable grazing are regarded to be outliers. This is in contrast to the bulk of the communities, which have not been able to preserve good rangeland.

In conclusion, An person, group, or community that is significantly different from the norm or that resides outside of a cluster of data points is referred to as an outlier.

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Label cells and extracellular matrix ECM

Answers

Cell- The fundamental units of all living organisms are cells. There are many billions of cells in a human body. They provide the body structure, absorb nutrients from meals, turn those into energy, and perform certain tasks.

Extracellular Matrix ECM- The extracellular matrix (ECM) is a complex network made up of several multidomain macromolecules arranged according to the cell/tissue in which they are found. The mechanical characteristics of tissues are influenced by the structural stability of the composite formed by the linking of ECM components.

Macromolecules- Any extremely big molecule, typically having a diameter between 100 and 10,000 angstroms ([tex]10^{-5}[/tex] to [tex]10^{-3}[/tex] mm). The smallest component of a material that nevertheless has its distinctive qualities is its molecule.

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What is the highest percentage of water vapor that can be in the air?

Question 1 options:

15%


4%


100%


60%

Answers

The answer is 4% since it ranges from 0.2% to 4%

Which of these sources is most likely to be credible when it comes to information on the advantages and disadvantages of using plastics?

a catalog of plastic products
a company trying to sell plastic products
a group of scientists studying plastic products
a group of activists against using plastic products

Answers

The most credible source that gives the information on the advantages and disadvantages of using plastics is: a group of scientists studying plastic products.

Plastics are the synthetic polymers. These are used for various purposes like furniture, kitchen-wares, packaging materials, containers, etc. The material that make up the plastics are:  polyethylene, PVC, nylon, etc. Plastics are non-biodegradable in nature.

Scientists are people who do research and perform experiments about various scientific aspects. They may also discover new things. Scientists are the most credible source for the knowledge about plastics because they give facts based on their research and experiments.

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Answer:

C on edge

Explanation:

just did it <3

Enzymes take on very important shapes/conformations and require cofactors and coenzymes to properly fold. What are some ways you could use the information about enzymes shape and specificity to design a treatment against a bacterial infection?

Answers

The ways in which one could use the information about enzymes shape and specificity to design a treatment against a bacterial infection is by making the antibacterial to be able to denature it.

What is Enzyme?

This is referred to a biological catalyst which is involved in speeding up the rate of a reaction and is proteinous in nature.

Enzymes are substrate specific which is why they only bind to a substrate which has a similar shape and the production of antibacterial will employ the use of agents which will denature it. The change in shape will make it impossible for the bacteria to perform certain metabolic reactions thereby leading to its death.

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A common yard decoration during Halloween is the infamous scarecrow. Aside from
Halloween, scarecrows were used by farmers and intended to scare away birds from
seeds and growing crops. But do they really work? Explain how you could test if
scarecrows are truly an effective bird deterrent.

Answers

Yes, scarecrows work effectively as bird deterrents.

Scarecrows are human like figures that are stationed on fields or farmlands to scare away birds.

To know how effective scarecrow can be, the following things need to be done.

 The scarecrow has to flashy that it will attract attention from distance The position of the scarecrow has to changed often so that the birds will not get familiar with the position It should be adorned with accessories that make noise when there is breeze.

The scarecrow works as effective bird deterrents when it is not left the same for a long period of time.

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During the winter, many creatures are able to drastically decrease their body temperatures and energy use and enter a reduced state of metabolism. This state is known asA)camouflage.B)hibernation.C)hyperventilation.D)migration.

Answers

B.) Hibernation.

Hibernation is when animals during winter tend to stay still and reduce the metabolism. Is exactly what the parragraph states.

Within the active site of an enzyme, the amino acid side chains interact with the substrate. When the substrate binds, it causes a slight confirmational shift in the enzyme and active site, which induces what in the substrate?

Answers

When the substrate binds, it causes a slight confirmational shift in the enzyme and active site, which induces a slight change in shape in the substrate.

What is an enzyme?

An enzyme is defined as a type of protein that has the ability to increase the rate of a chemical reaction but remains unchanged at the end of the chemical reaction.

The enzyme consists of a binding site that is made up of amino acids where the substrate interacts.

A slight conformational shift occurs in the active site of the enzyme which automatically leads to the slight change in shape of the substrate and formation of substrate-enzyme complex.

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Do you believe humans evolved from apes? Why do you think that?

Answers

Answer:

NO

Explanation:

We do share a common ape ancestor with chimpanzees. It lived between 8 and 6 million years ago. But humans and chimpanzees evolved differently from that same ancestor. All apes and monkeys share a more distant relative, which lived about 25 million years ago.

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Chimpanzees and other living big apes are not the ancestors of humans. Instead, our ancestor was a person like you and me who lived around 10 million years ago.

Chimpanzees and we do have a shared ape ancestry. Between 8 and 6 million years ago, it was alive. However, chimpanzees and humans diverged from the same progenitor in distinct ways. A more distant cousin of all apes and monkeys existed around 25 million years ago. Researchers believe that as early humans began to spend more time on the ground, they started to set themselves apart from early chimpanzees. Isbell speculated that when our ancestors explored new environments, they could have been on the lookout for food. Our common ancestor with apes, who lived and perished in the distant past, is where we originated and descended from. This proves that not only are we apes ourselves, but also that we are linked to other apes. The other current ape species, who coexist with us in the wild and in zoos, have also descended from that same common ancestor.

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What can strong CMEs affect on the earth 

Answers

Answer:

CMEs can also cause surges in electrical currents which overload power grids causing widespread blackouts. Also, according to NASA, CMEs can jostle Earth's magnetic field which can impair radio transmissions and increase radio static in Earth's ionosphere.

the structural cells of the nervous system are called

Answers

Neurons are the structural and functional units of the nervous system.

Numerous millions of neurons make up the neurological system. An internal body cell known as a neuron, also known as a nerve cell, is responsible for receiving, processing, and transmitting information via electrical and chemical impulses.

The brain, where information is processed, is the central component of the nervous system and contains the majority of its neurons. A cell body, also known as soma, dendrites, and an axon are components of every neuron.

There are only two primary types of cells in nerve tissue, despite the fact that the nervous system is incredibly complicated. The neuron is a type of nerve cell. The nervous system's basic building block, the "conducting" cell sends impulses. The other kind of cell is a neuroglia, sometimes known as a glial cell. "Neuroglia" translates as "nerve glue."

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The structural cells of the nervous system are called neurons and nerve cells.

What are neurons?

The structural and operational components of the nervous system are called neurons. The neurological system is made up of several millions of neurons. A neuron, also referred to as a nerve cell, is an internal body cell that is in charge of receiving, processing, and sending information via electrical and chemical impulses.

The majority of the nervous system's neurons are found in the brain, which serves as the central hub for processing information. Each neuron has an axon, a cell body, often called a soma and dendrites.

Despite the fact that the nervous system is highly complex, there are just two main types of cells in nerve tissue.

Therefore, neurons and nerve cells are the structural cells of the nervous system.

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What organelle functions are not important or could be done by another organelle in a cell? (Don’t say that they’re all important even though they are)

Answers

Answer:Cytoplasm. The cytoplasm is present both in plant and animal cells.

Explanation:

What function is shared by both the skeletal system and the muscular system?



warming the body


protecting vital organs


creating red blood cells


fighting bacterial infections

Answers

Protecting vital organs because the skeletal system builds a cage protecting your vital organs, as well as your muscles are on your bones/skeletal system.

Skeletal system and the muscular system help in protecting vital organs.

What is skeletal system?

The skeletal system aids in defending the body's essential organs. The body's bones and joints are all part of the skeletal system. Each bone contains several cells, protein fibers, and minerals to form a complex living organ.

The soft tissues that make up the rest of the body's soft tissues are supported and shielded by the skeleton, which serves as a scaffold. Muscles can link to the skeletal system at specific places to enable joint mobility.

One of the three important muscle tissues in the human body is found in the skeleton. Thousands of muscle fibers are encased in connective tissue sheaths to form each skeletal muscle. Fasciculi are the individual bundles of muscular fibers that make up skeletal muscles.

Therefore, Skeletal system and the muscular system help in protecting vital organs.

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